LOCUS       sequence-267803-        9787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of nSaCas9 intradomain insertion of single evolved TadA 
            monomer (V82G) at the specified position (E131 ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9787)
  AUTHORS   Nguyen Tran MT, Mohd Khalid MKN, Wang Q, Walker JKR, Lidgerwood GE, 
            Dilworth KL, Lisowski L, Pebay A, Hewitt AW
  TITLE     Engineering domain-inlaid SaCas9 adenine base editors with reduced 
            RNA off-targets and increased on-target DNA editing.
  JOURNAL   Nat Commun. 2020 Sep 25;11(1):4871. doi: 10.1038/s41467-020-18715-y.
  PUBMED    32978399
REFERENCE   2  (bases 1 to 9787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-09-25"; volume: "11(1):4871. doi"; pages: " 
            10.1038/s41467-020-18715-y"
COMMENT     Sequence Label: nSaCas9 E131 miniABEMax V82G
FEATURES             Location/Qualifiers
     source          1..9787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        130..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     758..777
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        758..776
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1195..1215
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1231..1251
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4666..4713
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4714..4737
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4747..4803
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4804..5202
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(5273..5290)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5279..5503
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5549..5977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5636..5655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5846..5867
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5986..6006)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5991..6320
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6171..6306
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6233..6252
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6387..7181
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6441..6460)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7051..7070
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7355..7476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7392..7411)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7446..7465
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7525..7541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7525..7541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7538..7560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7549..7565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7573..7579,7580..7597,7598..7603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7618..7639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7756..7773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7927..8515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8007..8026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8686..9477,9478..9546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9309..9328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9547..9651)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9726..9745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt
       61 gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat
      121 atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      721 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      781 cccaagctgg ctagcgccac catgaagcga aattacattc tggggctggc cattggcatt
      841 acatcagtgg gctatggcat cattgactac gagacaaggg acgtgatcga cgccggcgtg
      901 agactgttca aggaggccaa cgtggagaac aatgagggcc ggagatccaa gaggggagca
      961 aggcgcctga agcggagaag gcgccacaga atccagagag tgaagaagct gctgttcgat
     1021 tacaacctgc tgaccgacca ctccgagctg tctggcatca atccttatga ggccagagtg
     1081 aagggcctgt cccagaagct gtctgaggag gagtttagcg ccgccctgct gcacctggca
     1141 aagaggagag gcgtgcacaa cgtgaatgag gtggaggagg acaccggcaa cgagccgaag
     1201 aagaaacgta aggtgggtag cagcggtagc ccgaaaaaga agcgcaaagt gggcagcgac
     1261 gcgctggacg atttcgatct ggacatgctg ggcagcgatg cgctggatga tttcggcggt
     1321 ggcagctctg aggtggagtt ttcccacgag tactggatga gacatgccct gaccctggcc
     1381 aagagggcac gcgatgagag ggaggtgcct gtgggagccg tgctggtgct gaacaataga
     1441 gtgatcggcg agggctggaa cagagccatc ggcctgcacg acccaacagc ccatgccgaa
     1501 attatggccc tgagacaggg cggcctggtc atgcagaact acagactgat tgacgccacc
     1561 ctgtacggca cattcgagcc ttgcgtgatg tgcgccggcg ccatgatcca ctctaggatc
     1621 ggccgcgtgg tgtttggcgt gaggaacgca aaaaccggcg ccgcaggctc cctgatggac
     1681 gtgctgcact accccggcat gaatcaccgc gtcgaaatta ccgagggaat cctggcagat
     1741 gaatgtgccg ccctgctgtg ctatttcttt cggatgccta gacaggtgtt caatgctcag
     1801 aagaaggccc agagctccac cgacggcggt agcggcggta gcggtggtag cggcggcagc
     1861 ggtggtagcg gtggcagcgg tggtgagctg tccacaaagg agcagatcag ccgcaattcc
     1921 aaggccctgg aggagaagta tgtggccgag ctgcagctgg agcggctgaa gaaggatggc
     1981 gaggtgaggg gctccatcaa tcgcttcaag acctctgact acgtgaagga ggccaagcag
     2041 ctgctgaagg tgcagaaggc ctaccaccag ctggatcagt cctttatcga tacatatatc
     2101 gacctgctgg agacaaggcg cacatactat gagggaccag gagagggctc tcccttcggc
     2161 tggaaggaca tcaaggagtg gtacgagatg ctgatgggcc actgcaccta ttttccagag
     2221 gagctgagaa gcgtgaagta cgcctataac gccgatctgt acaacgccct gaatgacctg
     2281 aacaacctgg tcatcaccag ggatgagaac gagaagctgg agtactatga gaagttccag
     2341 atcatcgaga acgtgttcaa gcagaagaag aagcctacac tgaagcagat cgccaaggag
     2401 atcctggtga acgaggagga catcaagggc taccgcgtga cctccacagg caagccagag
     2461 ttcaccaatc tgaaggtgta tcacgatatc aaggacatca cagcccggaa ggagatcatc
     2521 gagaacgccg agctgctgga tcagatcgcc aagatcctga ccatctatca gagctccgag
     2581 gacatccagg aggagctgac caacctgaat agcgagctga cacaggagga gatcgagcag
     2641 atcagcaatc tgaagggcta caccggcaca cacaacctga gcctgaaggc catcaatctg
     2701 atcctggatg agctgtggca cacaaacgac aatcagatcg ccatctttaa ccggctgaag
     2761 ctggtgccaa agaaggtgga cctgtcccag cagaaggaga tcccaaccac actggtggac
     2821 gatttcatcc tgtctcccgt ggtgaagcgg agcttcatcc agagcatcaa agtgatcaac
     2881 gccatcatca agaagtacgg cctgcccaat gatatcatca tcgagctggc cagggagaag
     2941 aactccaagg acgcccagaa gatgatcaat gagatgcaga agaggaaccg ccagaccaat
     3001 gagcggatcg aggagatcat cagaaccaca ggcaaggaga acgccaagta cctgatcgag
     3061 aagatcaagc tgcacgatat gcaggagggc aagtgtctgt attctctgga ggccatccct
     3121 ctggaggacc tgctgaacaa tccattcaac tacgaggtgg atcacatcat cccccggagc
     3181 gtgagcttcg acaattcttt taacaataag gtgctggtga agcaggagga gaacagcaag
     3241 aagggcaata ggaccccttt ccagtacctg tctagctccg attctaagat cagctacgag
     3301 acattcaaga agcacatcct gaatctggcc aagggcaagg gccgcatcag caagaccaag
     3361 aaggagtacc tgctggagga gcgggacatc aacagattct ccgtgcagaa ggacttcatc
     3421 aaccggaatc tggtggacac cagatacgcc acacgcggcc tgatgaatct gctgcggtct
     3481 tatttcagag tgaacaatct ggatgtgaag gtgaagagca tcaacggcgg cttcacctcc
     3541 tttctgcgga gaaagtggaa gtttaagaag gagcgcaaca agggctataa gcaccacgcc
     3601 gaggatgccc tgatcatcgc caatgccgac ttcatcttta aggagtggaa gaagctggac
     3661 aaggccaaga aagtgatgga gaaccagatg ttcgaggaga agcaggccga gagcatgccc
     3721 gagatcgaga cagagcagga gtacaaggag attttcatca cacctcacca gatcaagcac
     3781 atcaaggact tcaaggacta caagtattct cacagggtgg ataagaagcc caaccgcgag
     3841 ctgatcaatg acaccctgta tagcacacgg aaggacgata agggcaatac cctgatcgtg
     3901 aacaatctga acggcctgta cgacaaggat aatgacaagc tgaagaagct gatcaacaag
     3961 tctcccgaga agctgctgat gtaccaccac gatcctcaga catatcagaa gctgaagctg
     4021 atcatggagc agtacggcga cgagaagaac ccactgtata agtactatga ggagacaggc
     4081 aactacctga caaagtatag caagaaggat aatggccccg tgatcaagaa gatcaagtac
     4141 tatggcaaca agctgaatgc ccacctggac atcaccgacg attaccctaa ctctcgcaat
     4201 aaggtggtga agctgagcct gaagccatac cggttcgacg tgtacctgga caacggcgtg
     4261 tataagtttg tgacagtgaa gaatctggat gtgatcaaga aggagaacta ctatgaggtg
     4321 aacagcaagt gctacgagga ggccaagaag ctgaagaaga tcagcaacca ggccgagttc
     4381 atcgcctctt tttacaacaa tgacctgatc aagatcaatg gcgagctgta tagagtgatc
     4441 ggcgtgaaca atgatctgct gaacagaatc gaagtgaata tgatcgacat cacctacagg
     4501 gagtatctgg agaacatgaa tgataagagg ccccctcgca tcatcaagac catcgcctct
     4561 aagacacaga gcatcaagaa gtacagcaca gacatcctgg ggaacctgta tgaagtcaag
     4621 agcaagaaac atcctcagat tatcaagaaa ggctctggtg gttctaagcg acctgccgcc
     4681 acaaagaagg ctggacaggc taagaagaag aaagattaca aagacgatga cgataaggga
     4741 tccggcgcaa caaacttctc tctgctgaaa caagccggag atgtcgaaga gaatcctgga
     4801 ccgatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca
     4861 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac
     4921 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc
     4981 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc
     5041 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat
     5101 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg
     5161 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagaattctg cagatatcca
     5221 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
     5281 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5341 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5401 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5461 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5521 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5581 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5641 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5701 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5761 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5821 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5881 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5941 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     6001 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     6061 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6121 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6181 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6241 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6301 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6361 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     6421 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     6481 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     6541 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     6601 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     6661 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     6721 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     6781 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     6841 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     6901 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     6961 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     7021 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     7081 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     7141 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     7201 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     7261 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7321 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7381 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7441 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7501 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7621 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7681 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8581 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8641 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8701 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8761 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8821 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8881 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8941 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9001 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9061 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9121 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9181 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9241 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9301 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9361 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9421 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9481 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9541 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9601 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9661 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     9721 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     9781 ttggagg
//
