LOCUS       sequence-267813-        9349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of human KIF16B 458-1317.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9349)
  AUTHORS   Yang R, Bostick Z, Garbouchian A, Luisi J, Banker G, Bentley M
  TITLE     A novel strategy to visualize vesicle-bound kinesins reveals the 
            diversity of kinesin-mediated transport.
  JOURNAL   Traffic. 2019 Aug 28. doi: 10.1111/tra.12692.
  PUBMED    31461551
REFERENCE   2  (bases 1 to 9349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic. 
            2019 Aug 28. doi: 10.1111/tra.12692."
COMMENT     Sequence Label: pBa.GFP-KIF16B tail 458-1317
FEATURES             Location/Qualifiers
     source          1..9349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..276
                     /label=chicken beta-actin promoter
     primer_bind     1219..1241
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             join(1309..1311,1312..1314,1315..2025)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1354..1375)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1615..1634)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1962..1983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4768..4902
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4805..4824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4859..4878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4933..4949)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4933..4949)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4946..4968)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4957..4973
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4981..4987,4988..5005,5006..5011))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5026..5047
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5164..5181)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5335..5923)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5415..5434)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6094..6885,6886..6954))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6717..6736
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6955..7059)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7127..7145
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7183..7205)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7305..7324
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7518..7540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7532..7549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7533..7549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      181 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      241 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc
      301 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
      361 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
      421 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
      481 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
      541 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
      601 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
      661 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
      721 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
      781 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
      841 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
      901 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
      961 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     1021 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     1081 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1141 gccgtcccct tctccatctc cagcctcggg gctgtccgca gggggacggc tgccttcggg
     1201 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggggtt tatatcttcc
     1261 cttctctgtt cctccgcagc ccccaagctt ggcgcgccta ggcctaccat ggtgagcaag
     1321 ggcggggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     1381 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     1441 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1501 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1561 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1621 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1681 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1741 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1801 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1861 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1921 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1981 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg aggatccgga
     2041 ggcggctctg gcggagggat cgatgatgac cttttgagta ctggaatcat cttatatcat
     2101 ttaaaggaag gtcagacata cgttggtaga gacgatgctt ccacggagca agatattgtt
     2161 cttcatggcc ttgacttgga gagtgagcat tgcatctttg aaaatatcgg ggggacagtg
     2221 actctgatac ccctgagtgg gtcccagtgc tctgtgaatg gtgttcagat cgtggaggcc
     2281 acacatctaa atcaaggtgc tgtgattctc ttgggaagaa ccaatatgtt tcgctttaac
     2341 catccaaagg aagccgccaa gctcagggag aagaggaaga gtggccttct gtcctccttc
     2401 agcttgtcca tgaccgacct ctcgaagtcc cgtgagaacc tgtctgcagt catgttgtat
     2461 aaccccggac ttgaatttga gaggcaacag cgtgaagaac ttgaaaaatt agaaagtaaa
     2521 aggaaactca tagaagaaat ggaggaaaag cagaaatcag acaaggctga actggagcgg
     2581 atgcagcagg aggtggagac ccagcgcaag gagacagaaa tcgtgcagct ccagattcgc
     2641 aagcaggagg agagcctcaa acgccgcagc ttccacatcg agaacaagct aaaggattta
     2701 cttgcggaga aggaaaaatt tgaagaggag aggctgaggg aacagcagga aatcgagctg
     2761 cagaagaaga gacaagaaga agagaccttt ctccgcgtcc aagaagaact ccaacgactc
     2821 aaagaactca acaacaacga gaaggctgag aagtttcaga tatttcaaga actggaccag
     2881 ctccaaaagg aaaaagatga acagtatgcc aagcttgaac tggaaaaaaa gagactagag
     2941 gagcaggaga aggagcaggt catgctcgtg gcccatctgg aagagcagct ccgagagaag
     3001 caggagatga tccagctcct gcggcgtggg gaggtacagt gggtggaaga ggagaagagg
     3061 gacctggaag gcattcggga atccctcctg cgggtgaagg aggctcgtgc cggaggggat
     3121 gaagatggcg aggagttaga aaaggctcaa ctgcgtttct tcgaattcaa gagaaggcag
     3181 cttgtcaagc tagtgaactt ggagaaggac ctggttcagc agaaagacat cctgaaaaaa
     3241 gaagtccaag aagaacagga gatcctagag tgtttaaaat gtgaacatga caaagaatct
     3301 agattgttgg aaaaacatga tgagagtgtc acagatgtca cggaagtgcc tcaagatttc
     3361 gagaaaataa agccagtgga gtacaggctg caatataaag aacgccagct acagtacctc
     3421 ctgcagaatc acttgccaac tctgttggaa gaaaagcaga gagcatttga aattcttgac
     3481 agaggccctc tcagcttaga caacactctt tatcaagtag aaaaggaaat ggaagaaaaa
     3541 gaagaacagc ttgcacagta ccaggccaat gcaaaccagc tgcaaaagct ccaagccacc
     3601 tttgaattca ctgccaacat tgcacgtcag gaggaaaaag tgaggaaaaa ggaaaaggag
     3661 attttggagt ccagagagaa gcagcagaga gaggcgctgg agcgggccct ggccaggctg
     3721 gagaggagac attctgcgct gcagaggcac tccaccctgg gcacggagat tgaagagcag
     3781 aggcagaaac ttgccagtct gaacagtggc agcagagagc agtcagggct ccaggctagc
     3841 ctggaggctg agcaggaagc cctggagaag gaccaggaga ggttagaata tgaaatccag
     3901 cagctgaaac agaagattta tgaggtcgat ggtgttcaaa aagatcatca tgggaccctg
     3961 gaagggaagg tggcttcttc cagcttgcca gtcagtgctg aaaaatcaca cctggttccc
     4021 ctcatggatg ccaggatcaa tgcttacatt gaagaagaag tccaaagacg ccttcaggat
     4081 ttgcatcgtg tgattagtga aggctgcagt acatctgcag acacgatgaa ggataatgag
     4141 aaacttcaca atggcaccat tcaacgtaaa ctaaaatatg agcggatggt ttctcgctct
     4201 ttgggcgcaa atccagatga cctgaaggac ccaattaaaa ttagtatccc acgctacgtc
     4261 ctctgcgggc aaggaaagga tgcacacttc gagtttgagg tcaagattac tgtcctagat
     4321 gagacatgga ctgtattcag gcgttacagt cgttttcgag aaatgcataa aacattgaag
     4381 ttaaagtatg cagagcttgc tgcccttgaa tttcctccaa agaaactatt tggaaataag
     4441 gatgaacgtg tgattgctga gagacgaagt cacttagaga aatacctcag ggactttttc
     4501 agcgtgatgc tccagtccgc aacatctccc ctccacatca acaaagtggg actgactctc
     4561 tcgaaacata ccatttgtga gttttcacca ttcttcaaga aaggagtctt tgactacagc
     4621 agccacggga cggggcggac cgtctaatct agataagtaa tgatcataat cagccatatc
     4681 acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4741 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4801 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4861 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccccgggta ccgagctcga
     4921 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4981 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     5041 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     5101 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5161 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5221 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5281 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5341 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5401 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5461 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5521 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5581 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5641 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5701 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5761 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5821 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5881 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5941 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     6001 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6061 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     6121 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     6181 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     6241 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     6301 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     6361 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     6421 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6481 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6541 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6601 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6661 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6721 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6781 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6841 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6901 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6961 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7021 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7081 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     7141 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7201 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7261 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     7321 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     7381 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     7441 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7501 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagttggg
     7561 atctttgcat tggcccacgg ctctcaggat ggggatgctc cccttcagca cccggttccc
     7621 cttggaaact gatggtcctg gctctgtggc atggcagtgg cactgtgagg agccccctac
     7681 cagcagcaca cagtggggtt ggcactgcca cgctccggat gccgcgctct gatccaaccc
     7741 cataatcaag ggaacccgaa ttgccccatc attgccccca ccacccccat cctgccgggc
     7801 cctcacaccc cacgctgcct tctggtgaca ttccccagcc caaacccacg gcttcatggc
     7861 taccgcgggg catttcccat tgccgcccca ttatcagctc tgcacacctc ccgctgtacc
     7921 catgcctcgt ggctgccctt ctttgacgta taatcttcta attaataccc ggccttgtca
     7981 aagtggagca caaacgttaa ttaattcccc agcaggcagg taattaacag tgtgactccc
     8041 tttttgctgc gagtggggct gatacagaga gatgtggcac tatggagccc acggggtcct
     8101 ggcactgggt gcccacggag gtccccatgt gctgcagtgt caccgcctcc gaggagacag
     8161 tattgtccct gcggtgtccc tgcagctcag ctctgtccac agggccacct ccagtttgga
     8221 ggggacacaa tgcagccccg atgcaaccca tcctcgcagc atcccaggga caaagacccc
     8281 actgcaagac cgcacacagg gctgggtccc gctcccctaa tatctacagt gcttcttgca
     8341 tggcccctta atcaatgcag ttaatcagca tgcgctcatg caccgctctg gagctgcaaa
     8401 gcccctcgca gcgctgctca ccaacaccgc gcaccgcccc ggcccagcct gcagcacgcg
     8461 ctgcaaacag gaaagaaaca aaatattgcc caaatgtagg caaaggcatt cggctgcctt
     8521 gacctccgcc gggccgggcc ctgcctgact cagctcctta ctcagcgctc gcttcctccc
     8581 tccggctgcc accgccgcag cgcacaccct gacaaagagt ggcccttaac gggctctgag
     8641 gtgcacccag cagtgcactc agcagtccaa gggccggcct ggaggtttgc accgctacgt
     8701 gctgacatta gcattgaact tggccctggg tagtgctgca ggccgggcgg ggtgggtgta
     8761 gagagtgcag cgcgcgttgc acccggtgcc ccttcccctc ccttgcatcc cagcaggctg
     8821 caccccagca ccaggcccgt gcatgcatgc tcctggtgtt attgcagcct ggtgcatgca
     8881 tgcgtcttga tggtgcagcg ctgtgcatgc atcctccttg gtgtgtagca gtctagtgca
     8941 tgcatacccc tcggtgttat tgctgctctg tgcacgcacg ctcattgtat cacttcatcc
     9001 cagtgcatgc actcacactg gagcgattgc tgctcggtgc acgcacactc attgtatcac
     9061 tgcagctcag tggctgcacg cacaccggtg ttattgctgc tcggtgcgtg catgcacatc
     9121 agtgtcgctg cagctcagtg catgcacgct cattgcccat cgctatccct gcctctcctg
     9181 ctggcgctcc ccgggaggtg acttcaaggg gaccgcagga ccacctcggg ggtgggggga
     9241 gggctgcaca cgcggacccc gctccccctc cccaacaaag cactgtggaa tcaaaaaggg
     9301 gggagggggg atggaggggc gcgtcacacc cccgccccac accctcacc
//
