LOCUS       sequence-267994-        1273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgSIK2-A
FEATURES             Location/Qualifiers
     source          1..1273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        74..149
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    197..314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        413..624
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             678..701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             708..728
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cggttatttc ggatttcttg gctttatata tcttgtggaa ggacgaaaca ccgctacgac
       61 atcgagggca cgcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      121 aacttgaaaa agtggcaccg agtcggtgct tttttgaatt ctagatcttg agacaaatgg
      181 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      241 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      301 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggcaaa
      361 ccgctgttcc taggaatccc gaggccttcg agtggctccg gtgcccgtca gtgggcagag
      421 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
      481 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
      541 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
      601 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga cccgacatta gcgctacagc
      661 ttaagcgggc caccatggat tacaaagacg atgacgataa gatggcccca aagaagaagc
      721 ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca
      781 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaagtgc ccagcaagaa
      841 attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga tcggagccct
      901 gctgttcgac agcggcgaaa cagccgagcc acccggctga gagaaccgcc agaggagata
      961 caccagacgg aagaccggat ctgctatctg cagagatctt cagcacgaga tggccaagtg
     1021 gacgacagct tcttcacaga ctgagagtct cctggtgaag agataagagc acgagcgcac
     1081 cccatcttcg cacatctgac gagtgctacc acgagagtac ccacatctac actgagagag
     1141 aactggtgaa gcaccgacng ncgacttgcg gcttgatcta tctggctgac caatgaatca
     1201 gttcgnnnnt tcgtatcagg nacnnaacga cacagcannt gtacannntt ctcacacacg
     1261 ctgtgtgtgc gaa
//
