LOCUS       sequence-268010-         637 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human BRSK1-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgBRSK1
FEATURES             Location/Qualifiers
     source          1..637
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        68..143
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        205..416
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      435..444
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgtaccgatt tcttggcttt atatatcttg tggaaggacg aaacaccggg tgagctattc
       61 gactaccgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      121 aaaaagtggc accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg
      181 gaattggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt
      241 tggggggagg ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg
      301 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
      361 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggacc
      421 ggttctagag cgctgccacc atggacaaaa agtacagcat cggcctggac atcggcacca
      481 actctgtggg ctgggccgtg atccccgacc aatacaaggt gcccaacaag aaattcaagg
      541 ggctgggcaa ccccgaccgg cacagcatca agaaaaacct gatcggaacc cctgctgttc
      601 gaaaagcggg taaacagccg aggccccccg gttgaaa
//
