LOCUS       sequence-268012-        1254 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human MARK1-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgMARK1
FEATURES             Location/Qualifiers
     source          1..1254
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        72..147
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        209..420
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      439..448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccgtatacg gattcttggc tttatatatc ttgtggaagg acgaaacacc gagtggctcc
       61 ttgcctttcg agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      121 cttgaaaaag tggcaccgag tcggtgcttt tttgaattcg ctagctaggt cttgaaagga
      181 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      241 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
      301 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      361 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      421 gaccggttct agagcgctgc caccatggac aagaagtaca gcatcggcct ggacatcggc
      481 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
      541 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
      601 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
      661 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
      721 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
      781 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      841 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
      901 ctgatctatc tggccctggc ccacatgatc aagttcccgg ggccacttcc tgatcgaggg
      961 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     1021 caaccagctg ttcgaggaaa ccccatcacg ccagcgcgtg acgccaagcc atcctgtctg
     1081 cagactgagc agagcagacg ctggaaatct gatcgccagc tgcccggcga aaagaagatg
     1141 nntgttcgaa actgatgccc tgacctggnn gaaccccact tcagagcacc tccgactnnc
     1201 gaggatgnan tgcagctgaa ccagnaccct acgacgacgn cctgtgaaac actc
//
