LOCUS       sequence-268028-        2214 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destabilized GFP reporter to monitor calcineurin activity via 
            calcineurin-dependent response element (CDRE) in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Diessl , J.,  Nandy, A., Schug, C., Habernig, L.,, Buttner, S.
  TITLE     Stable and destabilized GFP reporters to monitor calcineurin 
            activity in Saccharomyces cerevisiae.
  JOURNAL   Microbial Cell, 2020: in press
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbial 
            Cell, 2020: in press"
COMMENT     Sequence Label: pAMS366-4xCDRE-GFP-PEST
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             615..1328
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg
       61 gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga
      121 gaagatgcgg ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact
      181 cacaaattag agcttcaatt taattatatc agttattacc ctcgagcaag cgcacagcca
      241 ccgactggtg tcgagcaagc gcacagccac cgactggtgt cgaggtcgag caagcgcaca
      301 gccaccgact ggtgtcgagg tcgagcaagc gcacagccac cgactggtgt cgaggtcgag
      361 cagatccgcc aggcgtgtat atagcgtgga tggccaggca actttagtgc tgacacatac
      421 aggcatatat atatgtgtgc gacgacacat gatcatatgg catgcatgtg ctctgtatgt
      481 atataaaact cttgttttct tcttttctct aaatattctt tccttataca ttaggtcctt
      541 tgtagcataa attactatac ttctatagac acgcaaacac aaatacacac actaaattaa
      601 taatgaccgg atccatgtct aaaggtgaag aattattcac tggtgttgtc ccaattttgg
      661 ttgaattaga tggtgatgtt aatggtcaca aattttctgt ctccggtgaa ggtgaaggtg
      721 atgctactta cggtaaattg accttaaaat ttatttgtac tactggtaaa ttgccagttc
      781 catggccaac cttagtcact actttcggtt atggtgttca atgttttgcg agatacccag
      841 atcatatgaa acaacatgac tttttcaagt ctgccatgcc agaaggttat gttcaagaaa
      901 gaactatttt tttcaaagat gacggtaact acaagaccag agctgaagtc aagtttgaag
      961 gtgatacctt agttaataga atcgaattaa aaggtattga ttttaaagaa gatggtaaca
     1021 ttttaggtca caaattggaa tacaactata actctcacaa tgtttacatc atggctgaca
     1081 aacaaaagaa tggtatcaaa gttaacttca aaattagaca caacattgaa gatggttctg
     1141 ttcaattagc tgaccattat caacaaaata ctccaattgg tgatggtcca gtcttgttac
     1201 cagacaacca ttacttatcc actcaatctg ccttatccaa agatccaaac gaaaagagag
     1261 accacatggt cttgttagaa tttgttactg ctgctggtat tacccatggt atggatgaat
     1321 tgtacaaagc atccaacttg aacatttcga gaaagcttac catatcaacc ccatcatgct
     1381 ctttcgaaaa ttcaaatagc acatccattc cttcgcccgc ttcctcatct caaagccaca
     1441 ctccaatgag aaacatgagc tcactctctg ataacagcgt tttcagccgg aatatggaac
     1501 aatcatcacc aatcactcca agtatgtacc aatttggtca gcagcagtca aacagtatat
     1561 gtggtagcac cgttagtgtg aatagtctgg tgaatacaaa taacaaacaa aggatctacg
     1621 aacaaatcac gggtcctaac agcaataacg caaccaatga ttatattgat ttgctaaacc
     1681 taaatgagtc taacaaggaa aaccaaaatc ccgcaacggc gcattacctc aatgggggcc
     1741 cacccaagac aagcttcatt aaccatggaa tgttcccctc gccaactggg accataaata
     1801 gcggtaaatc tagcagtgcc tcatctttaa tttcttttgg tatgggcaat acccaagtaa
     1861 tatagggatc cggagcttgg ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     1921 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1981 gacaggtttc ccgacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2041 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2101 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
     2161 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatg
//
