LOCUS       sequence-26807-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  TITLE     Largman Plasmids
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HOXD4-WV-MSCV
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(61..80)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(62..80)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(87..104)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(87..103)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(96..118)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      244..673
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(331..350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     541..562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgcctcccta cccggtagaa ttgctcgagc aattgctcga gcaattgctc gagcaattcg
       61 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
      121 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      181 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
      241 tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      601 tataagggat tttgccgatt tcggcctatt gtgttaaaaa atganntgat ttaacaaaaa
      661 tttaacgcgn nttttaaaca aaaaatattt agcgcttaga attt
//
