LOCUS       sequence-268078-       10559 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a 
            visual marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10559)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 10559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pTol2-CreLite
FEATURES             Location/Qualifiers
     source          1..10559
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(75..866,867..935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     698..717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(936..1040)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1108..1126
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(1136..1147,1148..1335)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     protein_bind    1407..1427
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4910..4934
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4944..4964
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7794..7850
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7851..8549
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             8559..8615
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8619..8639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9213..9237)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9266..9387
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9277..9296)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9331..9350
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9513..9675,9676..9687)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9704..9721)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9875..10463)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9955..9974)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
       61 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      121 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
      181 ttaccatctg gccccagtgc tgcaatgata ccgcgagatc cacgctcacc ggctccagat
      241 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
      301 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
      361 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
      421 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
      481 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
      541 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
      601 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
      661 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
      721 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
      781 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
      841 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
      901 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
      961 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     1021 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     1081 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtccagag
     1141 gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt tttggggatt
     1201 tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa ttacattttt
     1261 ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa agtacttatt
     1321 ttttggagat cacttcattc tattttccct tgctattacc aaaccaattg aattgcgctg
     1381 atgcccagtt taatttaaat agatctcaac tttgtataga aaagttgacc agcaaagttc
     1441 tagaatttgt cgaaacattt atgttatata tttcctgaaa aaaattctga gtaagttctt
     1501 aagtgttatt gccagcaaca taaacaacag acggcaaaat gaataaatga taacaaagca
     1561 gtaggcttaa ataaacctaa tttttatagg ctgttctcta caaccctcaa acagtgatta
     1621 gttttgtact tataaacttg ccctttcatt catatttcaa gaaaattggt tcagaagatc
     1681 tggatattct agcagttgtt caagctcatg gagggatcag tgacctgatt ccacaatgac
     1741 taggcctaat ccagaaatta gatgactgtc aacataaaaa ggcacagcac tcactagctg
     1801 ccctatatat tttattatat tttacatata ttattttatt tatttagctc tgagtgctgt
     1861 actttctggt taaagaaaac tgcttacaac agctaacctg tactacctca ggctcaggga
     1921 atttggaaca ggtttgtctg gtttgtttct ttaaccatgc atgcttgttt tcaactatgg
     1981 caacacagtc acatgggaca ttacagaaat gatttgtcga tgacatgcga cttttcttta
     2041 ataaagcgca aagatcccaa aaagcaaact tttaacaaaa atcatataat tatattttca
     2101 atccagcttt gtagcaactt tgtgctgctg ttcactcagc aacagatagt cagtataagg
     2161 tcagtgtgtc tcaaagcagt gccatctgtt tcacacattg cgttctatat ataagtgtgc
     2221 tggttgacac gacactgtat aaggcctagg ctaaaacaca aacaatgtag aatgacactg
     2281 tgtttttttt gtaaacaaat gttgtttttg gttaaacatc tttgtgaaaa catcctcctg
     2341 tcatgtattt gctatattca aatgttaaac ccgtgcagaa tagaacatat acaaaaaaaa
     2401 acaacacaac acatttttaa acattattaa atatcaagta ttgctggcag ttctgtttct
     2461 gttttacagt accctttgcc acagttctcc gcttttcctg gtccagattc cacaagtctg
     2521 attcaccaat agcaaagcga ataaacaacc aaagcagcca atcactgctt gtagactgtc
     2581 ctgcgagacc ggcccattcc agcacattct ggaaacttcc tttatatgat aattataaat
     2641 acatttaaat tattgataca aaacatgtaa ttcctagaac ataaccatag caatcattag
     2701 ttttcagggt aattatgtat ttttaggatt tgactgcgga aagatctggt catgtgacgt
     2761 ctcatgaacg tcacggccct gggtttctat aaatacagta ggactctcga ccatcggcag
     2821 atttttcgaa gaagaagatc agtttcagga gccgtactgt tccgtttcaa cgcaaatatt
     2881 aacggtaaga gcgaatttcc tagtttgttt tcatgccatt ctttaaaacc atagcgtatt
     2941 actttaatta tagtaaactt tcgctttctt tattacaaga gacgttttgt gttgattctc
     3001 cgcggacatt ttcggtcaga caatcagaaa atgaccgcgg aggaccagta acttgcatta
     3061 cacgtaagtt aaatcttcgt gtattaaaat ggttaggttg ttaacgtcaa ataggttacc
     3121 gtgtttgcgt gtgatcaggt tggttttgtt agatttttgt cagtattttt aatttatttg
     3181 ttttagttta tttatttttt ttgctgaatc atagtttgtg aacaaagaac ccggatgtta
     3241 catacagtac agccgccatg ttacagagag ttataactta atcattttaa aaataatttt
     3301 gccttacttt tagtttgtca tgttgagaaa tgaggaaatg ttaaaatgag gaaatatcca
     3361 attaatttaa tatatcaaaa taatccatga ttacaatgca ctgaactgga gaaaattaag
     3421 atgttttcta gtgtcatgaa acaaatgtaa gagatgtaca ttgtagatgt tttatgtcaa
     3481 gaattggcta gttgatgcag catactggcg atactcagtt gtaataacag taacgttaca
     3541 tgttaataga ctactgagta tgctgttctg tctatgtatg ctctgtaagc tacgagaagg
     3601 acttttttaa acagtaaagg gtgcaatatt tttacaaatt gaattaaata aaggctgtct
     3661 attaagtaat atgcttgata tttttcttac ttgatcggaa ataagaaaaa atataaacgt
     3721 tgttgctcta aaaatcctag ttcagtttag ccaaccacaa ataccttttt gttcctccaa
     3781 cagttttttt ttcttctcta taatatttgg cagtctatag tactccaaat gtttccccac
     3841 agtctaacta attggtacag ccaaaatcat gacacttatt gcaataataa ttttggttca
     3901 ttggcattgt tgatagcctg tgccactaat atggtcgatt gatcatgctt caggaagaaa
     3961 actatattgt ttgatgtaag attattaaat cttcacctgc ttccattaca aactattccc
     4021 atcttattga attctggtat gtcttaaagg attagttcac ttcccaaatc aaaatttact
     4081 tagccttttt tcatccatga tccctttttt tcatcattaa tgaagaaatt gtttttgaaa
     4141 aagtttcaag atttttttct ctatattgtg gagcttgtta gtttaaaatt ccaaaatgca
     4201 atatgtggct tcaaatggtt ctaaatgatc ccagtcaagg aataacagtc ttatctaatg
     4261 aaaccattag acctttaaaa ataaaaaaaa taaaagtatt tatttttaaa tgactgagtg
     4321 attaagttga atttcagcgt ttccttactg tgtagaagtc cttccttact ggccccaccc
     4381 tttggttctc tgccaatctg ctacctaatg taatgttgtg gaacattatt attctttatt
     4441 tcttaatttt ttatttttta ttttaaaaca atgtaaactg cacagatgtg cagtttgttt
     4501 aaaatggcca atgctttgga aatgcatgac ataattagat ttcatgatgc acaaagccaa
     4561 atctcagagc ttgtgcaaaa tgagctatca tttcactagg taagacccta aattttcata
     4621 taggatcatt tggacaattt tgctgcaggt aaaatgcatt ctatagtcca ctgtcagcca
     4681 ttgttttgga tagtatttat ttttctctac aagtatagtc aatagttttc tattatttta
     4741 aaggtttgta acatttaagg gtgaccaaat gcaaagtaaa atttcatttt cgggtgaact
     4801 atctcgttta acatgggaga agtgcaaaac atacattatt ggctagaaca ttgtagtatt
     4861 ttttaaatgg aaatgtgtga ttgctaatct tactttgaat ttgtttacag caagtttgta
     4921 caaaaaagca ggctgccacc atgcccaaga agaagaggaa ggtggtttcc ggagtcgggg
     4981 gtagtggcgg tggccgtggc ggtggccgtg gcggagaaga agaaccgtcg tcaagtcaca
     5041 ctcctaataa ccgaagagga ggagaacaag ctcaatcgtc gggaacgaaa tctctcagac
     5101 caagaagcaa cactgaatca atgagcaaag caattcaaca gtacaccgtc gacgcaagac
     5161 tccacgccgt tttcgaacaa tccggcgaat cagggaaatc attcgactac tcacaatcac
     5221 tcaaaacgac gacgtacggt tcctctgtac ctgagcaaca gatcacagct tatctctctc
     5281 gaatccagcg aggtggttac attcagcctt tcggatgtat gatcgccgtc gatgaatcca
     5341 gtttccggat catcggttac agtgaaaacg ccagagaaat gttagggatt atgcctcaat
     5401 ctgttcctac tcttgagaaa cctgagattc tagctatggg aactgatgtg agatctttgt
     5461 tcacttcttc gagctcgatt ctactcgagc gtgctttcgt tgctcgagag attaccttgt
     5521 taaatccggt ttggatccat tccaagaata ctggtaaacc gttttacgcc attcttcata
     5581 ggattgatgt tggtgttgtt attgatttag agccagctag aactgaagat cctgcgcttt
     5641 ctattgctgg tgctgttcaa tcgcagaaac tcgcggttcg tgcgatttct cagttacagg
     5701 ctcttcctgg tggagatatt aagcttttgt gtgacactgt cgtggaaagt gtgagggact
     5761 tgactggtta tgatcgtgtt atggtttata agtttcatga agatgagcat ggagaagttg
     5821 tagctgagag taaacgagat gatttagagc cttatattgg actgcattat cctgctactg
     5881 atattcctca agcgtcaagg ttcttgttta agcagaaccg tgtccgaatg atagtagatt
     5941 gcaatgccac acctgttctt gtggtccagg acgataggct aactcagtct atgtgcttgg
     6001 ttggttctac tcttagggct cctcatggtt gtcactctca gtatatggct aacatgggat
     6061 ctattgcgtc tttagcaatg gcggttataa tcaatggaaa tgaagatgat gggagcaatg
     6121 tagctagtgg aagaagctcg atgaggcttt ggggtttggt tgtttgccat cacacttctt
     6181 ctcgctgcat accgtttccg ctaaggtatg cttgtgagtt tttgatgcag gctttcggtt
     6241 tacagttaaa catggaattg cagttagctt tgcaaatgtc agagaaacgc gttttgagaa
     6301 cgcagacact gttatgtgat atgcttctgc gtgactcgcc tgctggaatt gttacacaga
     6361 gtcccagtat catggactta gtgaaatgtg acggtgcagc atttctttac cacgggaagt
     6421 attacccgtt gggtgttgct cctagtgaag ttcagataaa agatgttgtg gagtggttgc
     6481 ttgcgaatca tgcggattca accggattaa gcactgatag tttaggcgat gcggggtatc
     6541 ccggtgcagc tgcgttaggg gatgctgtgt gcggtatggc agttgcatat atcacaaaaa
     6601 gagactttct tttttggttt cgatctcaca ctgcgaaaga aatcaaatgg ggaggcgcta
     6661 agcatcatcc ggaggataaa gatgatgggc aacgaatgca tcctcgttcg tcctttcagg
     6721 cttttcttga agttgttaag agccggagtc agccatggga aactgcggaa atggatgcga
     6781 ttcactcgct ccagcttatt ctgagagact cttttaaaga atctggtggt tctgccggtg
     6841 gctccggttc tggctccagc ggtggcagct ctggtgcgtc cggcacgggt actgcgggtg
     6901 gcactggcag cggttccggt actggctctg gcaaccggaa atggtttccc gcagaacctg
     6961 aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc
     7021 agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg
     7081 acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg
     7141 aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca
     7201 tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata
     7261 acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg
     7321 acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg
     7381 tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg
     7441 gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg
     7501 cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc
     7561 atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc tggtctggac
     7621 acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga
     7681 tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg
     7741 atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgatggaagc ggagccacga
     7801 acttctctct gttaaagcaa gcaggagatg ttgaagaaaa ccccgggcct atgagcgagc
     7861 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact
     7921 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     7981 aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct
     8041 acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct
     8101 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     8161 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     8221 tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca
     8281 ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc
     8341 tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg
     8401 ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg
     8461 aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc
     8521 tccctagcaa actggggcac aagcttaatg gaagcggagc cacgaacttc tctctgttaa
     8581 agcaagcagg agatgttgaa gaaaaccccg ggcctatgcc caagaagaag aggaaggtga
     8641 tgttcttacc aaccgattat tgttgcaggt taagcgatca agagtatatg gagcttgtgt
     8701 ttgagaatgg ccagattctt gcaaagggcc aaagatccaa cgtttctctg cataatcaac
     8761 gtaccaaatc gatcatggat ttgtatgagg cagagtataa cgaggatttc atgaagagta
     8821 tcatccatgg tggtggtggt gccatcacaa atctcgggga cacgcaggtt gttccacaaa
     8881 gtcatgttgc tgctgcccat gaaacaaaca tgttggaaag caataaacat gttgacggtg
     8941 gttctgccgg tggctccggt tctggctcca gcggtggcag ctctggtgcg tccggcacgg
     9001 gtactgcggg tggcactggc agcggttccg gtactggctc tggcctgact gtgcaccaaa
     9061 acctgcctgc cctccctgtg gatgccacct ctgatgaagt caggaagaac ctgatggaca
     9121 tgttcaggga caggcaggcc ttctctgaac acacctggaa gatgctcctg tctgtgtgca
     9181 gatcctgggc tgcctggtgc aagctgaact gaacccagct ttcttgtaca aagtggtgat
     9241 ggccggccgc ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact
     9301 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     9361 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     9421 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaga
     9481 tatcaagctt aaacaagaat ctctagtttt ctttcttgct tttactttta cttccttaat
     9541 actcaagtac aattttaatg gagtactttt ttacttttac tcaagtaaga ttctagccag
     9601 atacttttac ttttaattga gtaaaatttt ccctaagtac ttgtactttc acttgagtaa
     9661 aatttttgag tactttttac acctctgggc gctcttccgc ttcctcgctc actgactcgc
     9721 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9781 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9841 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9901 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9961 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    10021 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    10081 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    10141 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    10201 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    10261 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    10321 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    10381 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    10441 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    10501 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttc
//
