LOCUS       sequence-268088-       10202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of GFP (as control) tagged with C-terminal
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10202)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-GFP-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..10202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3408..3410,3411..3413,3414..4124)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3714..3733)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4061..4082
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4144..4168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4170..4211
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4218..4988
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(4994..5014)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    5043..5631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5096..5116)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5514..5525)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5645..6144
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5680..5703)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6077..6096
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6165..6764
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6165..6184)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6661..6681
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6902..7135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7207..7328
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7244..7263)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7298..7317
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7368..7503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7523..7542)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7524..7542)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7552..7569)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7552..7568)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7561..7583)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7710..8165
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7797..7816)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8007..8028
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8191..8295
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8296..8364,8365..9156)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9327..9915
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9816..9835
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10069..10086
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gtgagcaagg
     3421 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3481 gccacaagtt cagcgtgtct ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3541 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3601 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3661 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3721 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3781 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3841 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggcga
     3901 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3961 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4021 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4081 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccaag ggtgggcgcg
     4141 ccgacccagc tttcttgtac aaagtgggcg gcaagcccat ccccaacccc ctgctgggcc
     4201 tggacagcac cgctagcatc ccgctgctga acgctaaaca gattctggga cagctggacg
     4261 gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa tcagtacctg ctggatcgaa
     4321 tcggcgagct gaagagtggg gatgcttgca ttgcagaata tcagcaggca gggagaggaa
     4381 gcagagggag gaaatggttc tctccttttg gagctaacct gtacctgagt atgttttggc
     4441 gcctgaagcg gggaccagca gcaatcggcc tgggcccggt catcggaatt gtcatggcag
     4501 aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa atggcccaat gacctgtatc
     4561 tgcaggatag aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg
     4621 cacagatcgt cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg
     4681 tcaatcaggg ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg
     4741 ccgctatgct gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg
     4801 ctccatatct gtcacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga
     4861 tcattgggga caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc
     4921 tgctggaaca ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg
     4981 ccgaaaagta agccaacttt attatacata gttgatatcc agcacagtgg cggccgctcg
     5041 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     5101 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     5161 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     5221 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     5281 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5341 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5401 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5461 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5521 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5581 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaattc
     5641 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     5701 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     5761 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     5821 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     5881 gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc
     5941 ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga
     6001 aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     6061 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     6121 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     6181 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     6241 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     6301 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6361 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6421 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6481 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6541 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6601 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6661 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6721 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc
     6781 cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc ggtaccttta
     6841 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     6901 ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     6961 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7021 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7081 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7141 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7201 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     7261 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     7321 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     7381 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7441 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7501 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     7561 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7621 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7681 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     7741 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     7801 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     7861 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     7921 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     7981 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8041 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8101 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8161 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8221 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8281 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8341 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8401 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8461 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8521 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8581 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8641 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8701 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     8761 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     8821 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     8881 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8941 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9001 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9061 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9121 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9181 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9241 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9301 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9361 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9421 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9481 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9541 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9601 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9661 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9721 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     9781 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     9841 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     9901 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     9961 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10021 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10081 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10141 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10201 at
//
