LOCUS       sequence-26831-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Stelnicki EJ, Koemuves LG, Holmes D, Clavin W, Harrison MR, Adzick 
            NS, Largman C
  TITLE     The human homeobox genes MSX-1, MSX-2, and MOX-1 are differentially 
            expressed in the dermis and epidermis in fetal and adult skin.
  JOURNAL   Differentiation. 1997 Oct . 62(1):33-41.
  PUBMED    9373945
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Differentiation. 1997 Oct . 62(1):33-41."
COMMENT     Sequence Label: MSX2-pET
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             26..48
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             67..84
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             94..111
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             115..147
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gngnngnnnt tccctctaga ataattttgt ttaactttaa gaaggagata taccatgggc
       61 agcagccatc atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatggct
      121 agcatgactg gtggacagca aatgggtcgg atcctcatgg cttctccgac taaaggcggt
      181 gacttgtttt cgtcggatga ggagggcccc gcggtactgg ccggcccggg tcctgggcct
      241 ggaggagccg agggcagcgc agaggagcgc aggatcaagg tctccagcct gcccttcagc
      301 gtggaggcgc tcatgtccga caagaagccg cccaaggaat cgcccgcggt gccacccgac
      361 tgcgcctcgg ctggcgctgt cctgcggccg ctgctgctgc cgggacacgg cgtccgggac
      421 gctcacagtc ccgggcctct cgtcaagccc ttcgagaccg cctcggtcaa gtcggaaaat
      481 tccgaagacg gagcaccgtg gatacaggag cccggcagat actccccgcc gcccagacat
      541 atgagcccca ccacctgcac cctgaggaaa cacaagacca accggaagcc acgcacaccc
      601 ttcaccacat cccagcttct agccttggag cgcaagttcc gccagaaaca gtacctgtcc
      661 atagcagagc gggctgagtt ctccagctct ctgaacctta cagagaccca ngtcaaaatc
      721 tggttccaga accgaaaggg ctaangcgaa aagactgcaa gaggcggaac tggaaaagct
      781 gaaaatggct gccaagccta tgctgccctc aaggcttcag tctgcccntc cctatcaact
      841 cacccctgca a
//
