LOCUS       sequence-268373-        2055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VPR-FRB (NES) under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD881 VPR-FRB (NES)
FEATURES             Location/Qualifiers
     source          1..2055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             145..294
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             751..1107
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1126..1695
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 gattataagg atcacgatgg cgactacaag gaccacgata ttgactacaa agacgatgac
       61 gataacggta tggaggaact ttcacaggcc ctggctagta gtttttcagt gtctggtgtc
      121 ggtatccatg gcgttcccgg atcagacgca ttggacgatt ttgatctgga tatgctggga
      181 agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt
      241 gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac
      301 tctagaagtt ccggatctgt tggtagccag tacctgcccg acaccgacga ccggcaccgg
      361 atcgaggaaa agcggaagcg gacctacgag acattcaaga gcatcatgaa gaagtccccc
      421 ttcagcggcc ccaccgaccc tagacctcca cctagaagaa tcgccgtgcc cagcagatcc
      481 agcgccagcg tgccaaaacc tgccccccag ccttacccct tcaccagcag cctgagcacc
      541 atcaactacg acgagttccc taccatggtg ttccccagcg gccagatctc tcaggcctct
      601 gctctggctc cagcccctcc tcaggtgctg cctcaggctc ctgctcctgc accagctcca
      661 gccatggtgt ctgcactggc tcaggcacca gcacccgtgc ctgtgctggc tcctggacct
      721 ccacaggctg tggctccacc agcccctaaa cctacacagg ccggcgaggg cacactgtct
      781 gaagctctgc tgcagctgca gttcgacgac gaggatctgg gagccctgct gggaaacagc
      841 accgatcctg ccgtgttcac cgacctggcc agcgtggaca acagcgagtt ccagcagctg
      901 ctgaaccagg gcatccctgt ggcccctcac accaccgagc ccatgctgat ggaatacccc
      961 gaggccatca cccggctcgt gacaggcgct cagaggcctc ctgatccagc tcctgcccct
     1021 ctgggagcac caggcctgcc taatggactg ctgtctggcg acgaggactt cagctctatc
     1081 gccgatatgg atttctcagc cttgctgggc tctggcagcg gcagccggga ttccagggaa
     1141 gggatgtttt tgccgaagcc tgaggccggc tccgctatta gtgacgtgtt tgagggccgc
     1201 gaggtgtgcc agccaaaacg aatccggcca tttcatcctc caggaagtcc atgggccaac
     1261 cgcccactcc ccgccagcct cgcaccaaca ccaaccggtc cagtacatga gccagtcggg
     1321 tcactgaccc cggcaccagt ccctcagcca ctggatccag cgcccgcagt gactcccgag
     1381 gccagtcacc tgttggagga tcccgatgaa gagacgagcc aggctgtcaa agcccttcgg
     1441 gagatggccg atactgtgat tccccagaag gaagaggctg caatctgtgg ccaaatggac
     1501 ctttcccatc cgcccccaag gggccatctg gatgagctga caaccacact tgagtccatg
     1561 accgaggatc tgaacctgga ctcacccctg accccggaat tgaacgagat tctggatacc
     1621 ttcctgaacg acgagtgcct cttgcatgcc atgcatatca gcacaggact gtccatcttc
     1681 gacacatctc tgttttctgg ttctggttct ggttcaggat ccggtggtgg cgggtctgga
     1741 ggaggaggta gtggtggagg tggctctggt accatgcttg agatgtggca tgaaggcctg
     1801 gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg
     1861 gagcccttgc atgctatgat ggaacgggga ccccagactc tgaaggaaac atcctttaat
     1921 caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca
     1981 gggaatgtca aggacctcct ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc
     2041 tcaaagacta gatga
//
