LOCUS       sequence-26848-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK
  TITLE     The C-terminal half of human Ago2 binds to multiple GW-rich regions 
            of GW182 and requires GW182 to mediate silencing.
  JOURNAL   RNA. 2009 May . 15(5):804-13.
  PUBMED    19324964
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            May . 15(5):804-13."
COMMENT     Sequence Label: GST-TNR(aa1-204)
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             20..37
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     protein_bind    41..65
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(733..757)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      784..793
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(834..853)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggcactcctc aaatcggatc tggttccgcg tggatcatca acaagtttgt acaaaaaagc
       61 aggctcttta aaggaaccaa ttcagtcgac tggatctatg agagaattgg aagctaaagc
      121 taccaaagac gtagaaagaa atcttagcag ggatttagtg caagaagaag aacagttgat
      181 ggaagaaaag aaaaagaaaa aagacgacaa gaaaaagaag gaagctgctc aaaagaaggc
      241 cactgaacaa aaaatcaaag tgccagaaca gataaagccc agtgtaagcc agcctcagcc
      301 tgccaactct aataacggca cttccacagc aaccagcact aataataatg ccaagcgagc
      361 tacagccaac aatcagcagc cacagcagca gcagcaacag cagcagccgc agcagcagca
      421 gccacagcag cagccacagc cgcagccgca gcagcagcag ccacagcagc agccacaggc
      481 cttgcctcgg tatcctcgtg aagtacctcc acgatttcgc caccaggaac acaaacagct
      541 tctaaagagg ggtcagcatt ttcctgttat agcggcaaac cttggatctg ctgttaaggt
      601 gttaaacagc cagtcagaaa gcagtgcttt aacaaatcaa cagccacaaa ataacggaga
      661 ggtgcagaac agcaaaaacc agtcagatat aaaccacagt acttcaggat cggccgcact
      721 cgagatatct agacccagct ttcttgtaca aagtggttga tggccgctga ctgactaagc
      781 ttggccgcca tggcccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      841 catcacaaat ttcacaagat agaagcattt tcttaggtgc attctagtgt gtgtgggttg
      901 gtccaacctc acatcaagtg atatcctaat caatggtctg ggaaatcg
//
