LOCUS       sequence-268493-         236 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) 
            promoter with a CMV minimal promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD1029 ZF43x6-Compact(CMVMin)_EYFP
FEATURES             Location/Qualifiers
     source          1..236
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        110..148
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     198..217
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        198..216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agatctgaat tggagtgagg acgtgtagag tgaggacgtg cggagtgagg acgtgtagag
       61 tgaggacgtg cggagtgagg acgtgtagag tgaggacgtg ccctctagag gtaggcgtgt
      121 acggtgggag gtctatataa gcagagctga gctctctggc taactagaga acccactgct
      181 tactggctta tcgaaattaa tacgactcac tatagggagt ccccaagctg gctagc
//
