LOCUS       sequence-26861-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Steigemann P, Wurzenberger C, Schmitz MH, Held M, Guizetti J, Maar 
            S, Gerlich DW
  TITLE     Aurora B-mediated abscission checkpoint protects against 
            tetraploidization.
  JOURNAL   Cell. 2009 Feb 6. 136(3):473-84.
  PUBMED    19203582
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Feb 6. 136(3):473-84."
COMMENT     Sequence Label: pMyrPalm_mEGFP
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(113..132)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(114..132)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(177..380)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(206..226)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        381..684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ccgggnaaag ctcctcgccc ttgctcacct tcatgtccac gccgtcgtcg ttcaggttgt
       61 ccttgcgctt gctcttgatg cagcccatgg tggcggccgc aagcttgggt ctccctatag
      121 tgagtcgtat taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct
      181 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      241 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      301 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      361 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
      421 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
      481 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
      541 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
      601 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
      661 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
      721 gtanttgatt actattaata actaatgcat ggcggtaata cggttatcca cagaatcagg
      781 gatacgcagg aaaaacatgt gagcaaaggc acaaagccag gaccgtaaaa gcgcgtgctg
      841 ggtttccaag gtc
//
