LOCUS       sequence-268962-       18256 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a 
            Gateway cassette for cloning gRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18256)
  AUTHORS   Worthen JM, Yamburenko MV, Lim J, Nimchuk ZL, Kieber JJ, Schaller GE
  TITLE     Type-B response regulators of rice play key roles in growth, 
            development, and cytokinin signaling.
  JOURNAL   Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870.
  PUBMED    31160418
REFERENCE   2  (bases 1 to 18256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2019 Jun 3. pii: dev.174870. doi: 10.1242/dev.174870."
COMMENT     Sequence Label: pARS3_MUbCAS9_GW
FEATURES             Location/Qualifiers
     source          1..18256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..51
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     71..93
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3631..3655
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3674..3729
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             3737..7840
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3737..7840
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     RBS             5523..5531
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7841..7867
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(8134..8158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      8176..8883
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(8916..8934)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8917..8934)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    8974..9098
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(9123..9128,9129..9146,9147..9153)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             9207..9866
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(9273..9292)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             10208..10513
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10392..10411
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(10554..10678)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             10680..10706
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        10779..12487
                     /gene="RUBQ2"
                     /label=Ubi promoter
                     /note="promoter for the rice (Oryza sativa) polyubiquitin 
                     gene RUBQ2"
     intron          11526..12487
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     CDS             12722..13747
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13766..13940
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14053..14077
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      14157..14788
                     /label=oriV
                     /note="incP origin of replication"
     CDS             15000..15794
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     CDS             16093..17241
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     primer_bind     complement(17310..17327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17481..18069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17561..17580)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat
       61 agtttcgcgt agcggataac aatttcacac agggatatca ctagtaaaag gtacatttaa
      121 atcagttcga tgaccctagg ctgaactggt acggccggcc tcgatgctta cgcacgtgaa
      181 ttccggatcg cctgcaggga cttgacaatt gtaccagttt aaacaagttg taatgagttg
      241 ctggcctctc ttgggattgt tcatcaaagt agctgtccct acacaccaca acagaatggt
      301 gttgtggaga gaaagcacag acacatcctt gagatggcaa gggcacttaa gtttcagagt
      361 ggtgtaccta ccaggttttg gggagactgt gtcaagactg ttgtatacct gatcaataga
      421 ctccctactc caatcttaca aggcaaatgt ccatatgaac tactctatca gaaacatgcc
      481 aagcttgatc acttgagagt gtttgggtgt cttgcttttg cagcaacact gcccaaaggt
      541 gacaaacttg cacctagagc caagcgaacc atcttcattg gttattctga gactcaaaag
      601 ggttacaggt tgtatgactt ggataataag gtgatcatag tgagcaggga tgtagttttc
      661 agagagtttc agttcccttt caaagagtcc atctcccatg aacctgatat gttcactcga
      721 gcaggttcac ttagctctga agatacagct ttgcagctag tgtataatga tatttttcct
      781 tccagctttc atactgatga tgatgatact ccagttctta ttcctgacat tgtagaggcc
      841 aatgaggata tcactttggg gactcattct acccaaaatc atatcacttc agctgatgct
      901 gcaccttcag gccacctgca tgctcctgca gagtcagttg agtcagctga gcctgaccta
      961 gcaaatgctg aatttgagca ccatactgct gtagctgatc cctctctagt tccagcaaac
     1021 ctgcacacta gacctaaacg caatgcaggt cctcccatct ggctcaagga ctttgtgaca
     1081 ctgaacaaag gctctaggga tgttccatac cctatagcca actatgtttc ctatgatcac
     1141 ttgcctgttc actatcaagc ttatttgagt gctttctcca cttatactga acctaaatcc
     1201 ttcaaagaag cagctcaaga tgagaaatgg atggaggcca tgtcccttga gatacaggct
     1261 cttgaggata ataacacctg ggagattgtc cctttacccc ctggtaaaca gcctataggg
     1321 tccaaatggg tgtacaaaat taaatacaaa gctaatggtg aagttgacag gtttaaggca
     1381 aggctagtgg ccaagggata cactcagcaa gaaggccttg actaccatga aactttttct
     1441 ccagtggcca aaatggtcac tgtaaaagct gtcatatctg ttgctgcttc caagggctgg
     1501 ttccttttcc agatggatgt caacaatgct tttctacaag gtgacctcat ggaagaagtt
     1561 tatatgtctc tgcctcaggg ttttcacagc caaggggaga ccaaagtgtg caggctgttg
     1621 aatccctcta tggtttaaag caagcatcaa ggcagtggaa catcaagctt accactgtcc
     1681 ttatgcaggc tggttttatg caaagtgctt atgatcactc cttgtttacc aaaagagagg
     1741 ggactgacct tgtcataatc ctgatttatg tggatgatct gttaataaca ggcagcagca
     1801 atgttttgat ttcagaagca aaggcaaccc tgcatcagca tttcaagatg aaagatttgg
     1861 gagaactaaa atacttctta ggcattgagg tgctgagatc agaaaaggga atcctactga
     1921 accagaggaa gtatgcactg gaactgatat caggtgttgg tctgggaggc tgcagaccag
     1981 tgtcaacccc aatggaacag aaccaaaggc tgactactgt agaatatgac aagcacttag
     2041 gaaagactga tgatgcagag ttagaggatg tgggttccta tcagagactg gtgggaaagc
     2101 ttctctactt gacaatcaca aggccagata tcagctttgc agtgcaggtg ctgagtcaat
     2161 tcatgcagca acccaaacag tcacacctgg aagcagcatt gagggttgtg aagtacataa
     2221 aaggttctcc aggtgttagg aagcggccgc tgcagcgtcg cgtcgggcca agcgaagcag
     2281 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
     2341 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
     2401 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
     2461 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
     2521 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
     2581 acccgtcggc acctccgctt caaggtacgc cgctcgtcct cccccccccc ctctctacct
     2641 tctctagatc ggcgttccgg tccatggtta gggcccggta gttctacttc tgttcatgtt
     2701 tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac ggatgcgacc
     2761 tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg gaatcctggg
     2821 atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt tcgttgcata
     2881 gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt tgtcgggtca
     2941 tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg cggtcgttct
     3001 agatcggagt agaaatctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat
     3061 gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta
     3121 ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc
     3181 gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag
     3241 aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata
     3301 catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg
     3361 ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct
     3421 ctaaccttga gtacctatct attataataa acaagtatgt tttataatta ttttgatctt
     3481 gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc
     3541 atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg
     3601 ttacttctgc agggcgcgcc gatatcatca acaagtttgt acaaaaaagc aggctcttta
     3661 aagtattttt acaacaatta ccaacaacaa caaacaacaa acaacattac aattactatt
     3721 tacaattaca aaaaaaatgg acaaaaaata cagcattggg ttggacattg ggacaaatag
     3781 cgtgggttgg gcagtcatta cagacgaata caaggttcct agcaagaagt tcaaggtgtt
     3841 gggcaacact gacaggcact cgatcaagaa aaatctcatt ggtgcactcc ttttcgatag
     3901 tggggagact gccgaagcga ccagactgaa aaggactgct agaaggcgct atacccggcg
     3961 taagaatcgc atttgctacc tccaggagat attctctaac gaaatggcca aggttgatga
     4021 cagcttcttt catcgcttgg aggaatcatt cctcgtcgag gaagacaaga aacacgagcg
     4081 gcatccgatc tttggaaaca ttgttgatga ggtcgcatat cacgaaaagt accccacgat
     4141 ctaccatctt cgcaagaaac tggtggattc aactgacaag gctgatctgc ggttgatata
     4201 cctcgcactt gctcacatga tcaagttccg tggccatttt cttattgagg gagacctgaa
     4261 ccctgataat tctgacgtcg ataagttgtt catccagctc gtgcaaacat acaatcagct
     4321 ctttgaggaa aaccctatca acgcctctgg agttgatgcc aaagcgatcc tttccgcgag
     4381 gctgtcgaag agtagaaggc tggagaactt gatcgcccaa cttcctggcg aaaagaaaaa
     4441 cggtttgttc gggaatctca ttgcgctgag cttgggactc acgccaaact tcaagtctaa
     4501 cttcgacctc gcagaggatg ctaaacttca gctgtccaag gatacttatg atgacgatct
     4561 tgacaacctg ttggcacaaa tcggtgacca gtacgctgat ttgttcctcg ctgccaagaa
     4621 cctcagcgat gccatactcc tttcagacat cctccgcgtg aacactgaga ttaccaaagc
     4681 acctctgtct gcttccatga taaagcgtta tgatgagcac catcaagacc tcactctgtt
     4741 gaaggcgttg gttagacagc aactcccaga gaagtacaaa gaaatcttct ttgaccaatc
     4801 taagaatggc tacgccggat atattgatgg cggagcgtcc caggaggagt tctacaagtt
     4861 tatcaaacct attcttgaga agatggatgg tacggaggaa ctccttgtta aactgaatag
     4921 agaggatctc ttgcgcaagc aacggacttt cgacaacggc agcataccgc accagatcca
     4981 tctcggagag cttcacgcca tccttcgccg gcaggaagat ttctacccct ttctgaagga
     5041 caaccgtgag aagatcgaaa aaatccttac tttcagaatc ccttactatg tcggtccact
     5101 ggctagaggc aactctagat ttgcttggat gacacgcaag agtgaggaaa ctatcacccc
     5161 ttggaacttc gaggaagtgg ttgacaaggg cgcatcggct caaagtttca ttgagagaat
     5221 gactaatttt gataagaacc ttccgaacga gaaagtcctg cccaagcatt cactccttta
     5281 cgagtatttc acagtgtata acgaacttac gaaggtgaaa tacgttactg agggtatgcg
     5341 gaagccggca ttcctgtcgg gggaacaaaa gaaagctata gtggatctgt tgttcaaaac
     5401 taacaggaag gttaccgtca agcagcttaa ggaggattac ttcaagaaaa tagaatgttt
     5461 tgactcggtg gagatcagtg gcgttgaaga tagattcaac gcttcactgg gaacctacca
     5521 cgacctcctt aagatcatta aggacaaaga tttcttggat aacgaggaaa atgaggacat
     5581 tctcgaagat atagtcctta cactgacgtt gtttgaggac cgtgaaatga tcgaggaaag
     5641 attgaagacc tatgctcatc tcttcgacga taaggttatg aagcagctca agcgtagaag
     5701 gtacacaggt tgggggcgtc tttctagaaa gctgattaat ggcataaggg ataaacaatc
     5761 tggaaagacg attctggatt tcctcaagtc cgacggcttc gccaaccgca attttatgca
     5821 gcttatacac gacgattccc tgactttcaa agaggacatc cagaaggccc aagttagcgg
     5881 ccaaggagat tcacttcacg agcatatcgc caacctggcg ggtagcccgg cgatcaagaa
     5941 agggattctt cagaccgtca aggtcgtgga tgagctggtg aaagttatgg gccggcataa
     6001 gcccgaaaac atagtgatcg agatggcccg tgaaaatcag actacccaaa aaggacagaa
     6061 gaactcccgc gagcggatga aaaggataga ggaaggcatc aaggaacttg gaagccagat
     6121 cctgaaggag cacccagttg aaaacacaca gttgcaaaat gagaagctct atctttacta
     6181 tctccaaaat ggtagagaca tgtatgtgga tcaggagttg gacattaaca ggctctcaga
     6241 ctacgatgtc gaccatatcg tgccgcaatc gttccttaag gacgatagta tagataataa
     6301 ggtgctgact cggtcggata aaaaccgtgg caagtcggac aatgttccca gtgaggaagt
     6361 tgtgaagaag atgaagaact actggcgcca actgttgaat gcaaaattga tcacccagag
     6421 gaagtttgac aacctcacaa aagcagagcg cggtgggctc agtgaacttg ataaagctgg
     6481 attcattaag aggcaacttg ttgagacacg ccagattacg aagcacgtcg cgcagatact
     6541 ggattctagg atgaacacca agtacgacga gaatgataaa ttgatcagag aagtgaaggt
     6601 tatcacattg aagtctaaac tcgtgtccga ttttcgcaaa gacttccaat tttataaggt
     6661 tcgggagatc aacaattatc accatgcaca tgatgcttac ctcaacgccg tggttggtac
     6721 ggcgttgatt aagaaatacc cgaagctgga gtctgagttc gtgtacgggg attataaggt
     6781 ctacgacgtg aggaaaatga tcgctaagag tgagcaggaa attggtaaag ccaccgcgaa
     6841 gtatttcttt tactctaaca tcatgaactt cttcaagaca gagataacgt tggcaaatgg
     6901 tgaaatacgt aagagacctc tcatcgagac taacggtgaa accggggaaa tcgtttggga
     6961 taaagggcgg gactttgcta ctgttcgtaa ggtcctctcc atgccgcaag tgaatattgt
     7021 taagaaaaca gaggtccaga cgggcggatt ctctaaggaa tccatccttc ccaaaagaaa
     7081 cagcgacaag ctgattgcga ggaagaaaga ttgggaccct aagaaatatg gtgggttcga
     7141 ttctccaacc gttgcctact ccgtcttggt cgtggcgaag gttgagaagg gtaaatctaa
     7201 gaaactcaaa tccgtcaagg aactccttgg gattactatc atggagaggt cttccttcga
     7261 aaagaatcct atcgatttcc ttgaggctaa aggctataag gaagtgaaga aggatctcat
     7321 catcaagctc ccaaagtaca gcctgtttga gttggaaaac ggaaggaagc gcatgttggc
     7381 atcagctgga gagctccaga aagggaacga gctggccttg ccttctaagt atgtgaactt
     7441 cctctatctt gcgtcgcact acgagaagct gaaaggcagt ccagaggaca acgaacagaa
     7501 acaacttttt gttgagcaac acaagcatta tctggatgag attatagaac agatcagcga
     7561 gttctcaaaa agagtcattc ttgccgatgc taacctcgac aaggtgctct ccgcatacaa
     7621 caaacacaga gacaagccga tcagggagca ggctgaaaat atcattcatc tcttcactct
     7681 taccaacctg ggtgcccccg cggcattcaa gtattttgat acaacgattg accggaaacg
     7741 ttacacaagc acgaaggagg tccttgatgc gaccctgatt catcaaagca taacagggct
     7801 ctatgagacg agaatcgatt tgtcacagct cggcggagac tacccgtatg acgtgcccga
     7861 ttacgctgcc gcggcagctg agttcaagcg cgaggaacag gcccggaagg cgaaagtgaa
     7921 caatgagaag aaaacagaaa tcgttaaacc tgagagctgc tcaaacgagg gagacgttaa
     7981 ggatcttaag aggaaagact ctgaggatgg caacgaagga gaggaagagg aagctagctc
     8041 aaaacctaag aaaccaaagg tggcactctc acatctccaa gacattgacg acaccgaagc
     8101 agaccaagaa gaagaataac tcgagatatc tagacccagc tttcttgtac aaagtggttg
     8161 atgatatcta gagtcctgct ttaatgagat atgcgagacg cctatgatcg catgatattt
     8221 gctttcaatt ctgttgtgca cgttgtaaaa aacctgagca tgtgtagctc agatccttac
     8281 cgccggtttc ggttcattct aatgaatata tcacccgtta ctatcgtatt tttatgaata
     8341 atattctccg ttcaatttac tgattgtacc ctactactta tatgtacaat attaaaatga
     8401 aaacaatata ttgtgctgaa taggtttata gcgacatcta tgatagagcg ccacaataac
     8461 aaacaattgc gttttattat tacaaatcca attttaaaaa aagcggcaga accggtcaaa
     8521 cctaaaagac tgattacata aatcttattc aaatttcaaa agtgccccag gggctagtat
     8581 ctacgacaca ccgagcggcg aactaataac gctcactgaa gggaactccg gttccccgcc
     8641 ggcgcgcatg ggtgagattc cttgaagttg agtattggcc gtccgctcta ccgaaagtta
     8701 cgggcaccat tcaacccggt ccagcacggc ggccgggtaa ccgacttgct gccccgagaa
     8761 ttatgcagca tttttttggt gtatgtgggc cccaaatgaa gtgcaggtca aaccttgaca
     8821 gtgacgacaa atcgttgggc gggtccaggg cgaattttgc gacaacatgt cgaggctcag
     8881 caggaccggc atgcaagcta gcttactagt gatattctat agtgtcacct aaatctgcgg
     8941 ccgcaaagtc gacgaattct cccctcgaga tcaacaagtt tgtacaaaaa agctgaacga
     9001 gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact
     9061 acataatact gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag
     9121 gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccgtcga
     9181 gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg
     9241 atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta
     9301 cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata
     9361 agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg
     9421 aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt
     9481 acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg
     9541 atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg
     9601 cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga
     9661 gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca
     9721 ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc
     9781 atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact
     9841 gcgatgagtg gcagggcggg gcgtaaagat ctggatccgg cttactaaaa gccagataac
     9901 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     9961 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
    10021 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
    10081 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
    10141 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
    10201 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
    10261 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
    10321 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
    10381 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
    10441 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
    10501 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
    10561 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
    10621 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggtgt
    10681 acccatacga tgttccagat tacgcttgaa ctagtgggtg gatccaaggc cttctagaaa
    10741 aactcgagaa cgttgggccc caattggcta gcaagcttat tcgggtcaag gggaagccag
    10801 cgcgccaccc cacgtcagca aatacggagg cgcggggttg acggcgtcac ccggtcctaa
    10861 cggcgaccaa caaaccagcc agaagaaatt acagtaaaaa aaaagtaaat tgcactttga
    10921 tccacctttt attacctaag tctcaatttg gatcaccctt aaacctatct tttcaatttg
    10981 ggccgggttg tggtttggac taccatgaac aacttttcgt catgtctaac ttccctttca
    11041 gcaaacatat gaaccatata tagaggagat cggccgtata ctagagctga tgtgtttaag
    11101 gtcgttgatt gcacgagaaa aaaaaatcca aatcgcaaca atagcaaatt tatctggttc
    11161 aaagtgaaaa gatatgttta aaggtagtcc aaagtaaaac ttatagataa taaaatgtgg
    11221 tccaaagcgt aattcactca aaaaaaatca acgagacgtg taccaaacgg agacaaacgg
    11281 catcttctcg aaatttccca accgctcgct cgcccgcctc gtcttcccgg aaaccgcggt
    11341 ggtttcagcg tggcggattc tccaagcaga cggagacgtc acggcacggg actcctccca
    11401 ccacccaacc gccataaata ccagccccct catctcctct cctcgcatca gctccacccc
    11461 cgaaaaattt ctccccaatc tcgcgaggct ctcgtcgtcg aatcgaatcc tctcgcgtcc
    11521 tcaaggtacg ctgcttctcc tctcctcgct tcgtttcgat tcgatttcgg acgggtgagg
    11581 ttgttttgtt gctagatccg attggtggtt agggttgtcg atgtgattat cgtgagatgt
    11641 ttaggggttg tagatctgat ggttgtgatt tgggcacggt tggttcgata ggtggaatcg
    11701 tggttaggtt ttgggattgg atgttggttc tgatgattgg ggggaatttt tacggttaga
    11761 tgaattgttg gatgattcga ttggggaaat cggtgtagat ctgttgggga attgtggaac
    11821 tagtcatgcc tgagtgattg gtgcgatttg tagcgtgttc catcttgtag gccttgttgc
    11881 gagcatgttc agatctactg ttccgctctt gattgagtta ttggtgcctt gggttggtgc
    11941 aaacacaggc tttaatatgt tatatctgtt ttgtgtttga tgtagatctg tagggtagtt
    12001 cttcttagac atggttcaat tatgtagctt gtgcgtttcg atttgatttc atatgttcac
    12061 agattagata atgatgaact cttttaatta attgtcaatg gtaaatagga agtcttgtcg
    12121 ctatatctgt cataatgatc tcatgttact atctgccagt aatttatgct aagaactata
    12181 ttagaatatc atgttacaat ctgtagtaat atcatgttac aatctgtagt tcatctatat
    12241 aatctattgt ggtaatttct ttttactatc tgtgtgaaga ttattgccac tagttcattc
    12301 tacttatttc tgaagttcag gatacgtgtg ctgttactac ctatctgaat acatgtgtga
    12361 tgtgcctgtt actatctttt tgaatacatg tatgttctgt tggaatatgt ttgctgtttg
    12421 atccgttgtt gtgtccttaa tcttgtgcta gttcttaccc tatctgtttg gtgattattt
    12481 cttgcagatg cagatctttg tgaagacatt gaccggcaag actatcaccc tggaggtgga
    12541 gtcctctgac accatcgaca atgtcaaggc taagatccaa gataaggagg gcatcccccc
    12601 ggaccagcag cgtctcatct tcgctggcaa gcagctcgaa gatggcagga cccttgctga
    12661 ctacaacatc cagaaggagt ccacccttca ccttgtcctc cgcctccgtg gtggcggatc
    12721 catgaaaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
    12781 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
    12841 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
    12901 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
    12961 tggggagttt agcgagagcc tgacctattg catctcccgc cgttcacagg gtgtcacgtt
    13021 gcaagacctg cctgaaaccg aactgcccgc tgttctacaa ccggtcgcgg aggctatgga
    13081 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
    13141 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
    13201 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
    13261 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
    13321 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
    13381 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
    13441 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
    13501 acgactccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
    13561 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    13621 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    13681 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
    13741 gaaatagtta atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
    13801 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
    13861 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
    13921 ccagtactaa aatccagatc ccccgaatta attcggcgtt aattcagtac attaaaaacg
    13981 cgtacggtta aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct
    14041 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
    14101 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc actagagacg
    14161 ctcaccgggc tggttgccct cgccgctggg ctggcggccg tctatggccc tgcaaacgcg
    14221 ccagaaacgc cgtcgaagcc gtgtgcgaga caccgcagcc gccggcgttg tggatacctc
    14281 gcggaaaact tggccctcac tgacagatga ggggcggacg ttgacacttg aggggccgac
    14341 tcacccggcg cggcgttgac agatgagggg caggctcgat ttcggccggc gacgtggagc
    14401 tggccagcct cgcaaatcgg cgaaaacgcc tgattttacg cgagtttccc acagatgatg
    14461 tggacaagcc tggggataag tgccctgcgg tattgacact tgaggggcgc gactactgac
    14521 agatgagggg cgcgatcctt gacacttgag gggcagagtg ctgacagatg aggggcgcac
    14581 ctattgacat ttgaggggct gtccacaggc agaaaatcca gcatttgcaa gggtttccgc
    14641 ccgtttttcg gccaccgcta acctgtcttt taacctgctt ttaaaccaat atttataaac
    14701 cttgttttta accagggctg cgccctgtgc gcgtgaccgc gcacgccgaa ggggggtgcc
    14761 cccccttctc gaaccctccc ggcccgctct cgcgttggca gcatcaccca taattgtggt
    14821 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
    14881 gctgttttct ggtatttaaa gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
    14941 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
    15001 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
    15061 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
    15121 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
    15181 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg caccttgaac
    15241 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
    15301 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
    15361 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
    15421 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
    15481 aagacacccc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
    15541 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
    15601 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
    15661 ttgccttctg cgtccggtcg atcagggagg atattgggga agaacagtat gtcgagctat
    15721 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
    15781 atgaattgtt ttagtaccta gatgtggcgc aacgatgctg gcgacaagca ggagcgcacc
    15841 gacttcttcc gcatcaagtg ttttggctct caggccgagg cccacggcaa gtatttgggc
    15901 aaggggtcgc tggtattcgt gcagggcaag attcggaata ccaagtacga gaaggacggc
    15961 cagacggtct acgggaccga cttcattgcc gataaggtgg attatctgga caccaaggca
    16021 ccaggcggat caaatcagga ataagggcac attgccccgg cgtgagtcgg ggcaatcccg
    16081 caaggagggt gaatgaatcg gacgtttgac cggaaggcat acaggcaaga actgatcgac
    16141 gcggggtttt ccgccgagga tgccgaaacc atcgcaagcc gcaccgtcat gcgtgcgccc
    16201 cgcgaaacct tccagtccgt cggctcgatg gcccagcaag ctacggccaa gatcgagcgc
    16261 gacagcgtgc aactggctcc ccctgccctg cccgcgccat cggccgccgt ggagcgttcg
    16321 cgtcgtctcg aacaggaggc ggcaggtttg gcgaagtcga tgaccatcga cacgcgagga
    16381 actatgacga ccaagaagcg aaaaaccgcc ggcgaggacc tggcaaaaca ggtcagcgag
    16441 gccaagcaag ccgcgttgct gaaacacacg aagcagcaga tcaaggaaat gcagctttcc
    16501 ttgttcgata ttgcgccgtg gccggacacg atgcgagcga tgccaaacga cacggcccgc
    16561 tctgccctgt tcaccacgcg caacaagaaa atcccgcgcg aggcgctgca aaacaaggtc
    16621 attttccacg tcaacaagga cgtgaagatc acctacaccg gcgtcgagct gcgggccgac
    16681 gatgacgaac tggtgtggca gcaggtgttg gagtacgcga agcgcacccc tatcggcgag
    16741 ccgatcacct tcacgttcta cgagctttgc caggacctgg gctggtcgat caatggccgg
    16801 tattacacga aggccgagga atgcctgtcg cgcctacagg cgacggcgat gggcttcacg
    16861 tccgaccgcg ttgggcacct ggaatcggtg tcgctgctgc accgcttccg cgtcctggac
    16921 cgtggcaaga aaacgtcccg ttgccaggtc ctgatcgacg aggaaatcgt cgtgctgttt
    16981 gctggcgacc actacacgaa attcatatgg gagaagtacc gcaagctgtc gccgacggcc
    17041 cgacggatgt tcgactattt cagctcgcac cgggagccgt acccgctcaa gctggaaacc
    17101 ttccgcctca tgtgcggatc ggattccacc cgcgtgaaga agtggcgcga gcaggtcggc
    17161 gaagcctgcg aagagttgcg aggcagcggc ctggtggaac acgcctgggt caatgatgac
    17221 ctggtgcatt gcaaacgcta gggccttgtg gggtcagttc cggctggggg ttcagcagcc
    17281 agcgctttac tgagatcctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    17341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    17401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    17461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    17521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    17581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    17641 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    17701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    17761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    17821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    17881 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    17941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    18001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    18061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    18121 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg
    18181 gatctcctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    18241 tttaaatatc cgatta
//
