LOCUS       sequence-269092-       11192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK1 (human) tagged with C-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11192)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-BRSK1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..11192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5725..5749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5748..5789
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(5848..5867)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(5984..6004)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6033..6621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6086..6106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6504..6515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6635..7134
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6670..6693)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7067..7086
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7155..7754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7155..7174)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7651..7671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7892..8125
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8197..8318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8234..8253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8288..8307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8358..8493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8513..8532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8514..8532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8542..8559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8542..8558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8551..8573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8700..9155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8787..8806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8997..9018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9181..9285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9286..9354,9355..10146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9504..9523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10317..10905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10806..10825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11059..11076
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggcttaa tgtcgtccgg ggccaaggag ggaggtgggg
     3421 gctctcccgc ctaccacctc ccccaccccc acccccaccc accccagcac gcccaatatg
     3481 tgggccccta tcggctggag aagacgctgg gcaaaggaca gacagggctg gttaaactcg
     3541 gggtccactg catcacgggt cagaaggtcg ccatcaagat cgtgaaccgg gagaagctgt
     3601 cggagtcggt gctgatgaag gtggagcggg agatcgccat cctgaagctc atcgaacacc
     3661 cacatgtcct caagctccac gacgtctacg agaacaagaa atatttgtac ctggttctgg
     3721 agcacgtctc ggggggtgag ctattcgact acctggtaaa gaaggggaga ctgacgccca
     3781 aggaggcccg aaagttcttc cgccagattg tgtctgcgct ggacttctgc cacagctact
     3841 ccatctgcca cagagaccta aagcccgaga acctgctttt ggatgagaaa aacaacatcc
     3901 gcattgcaga cttcggcatg gcgtccctgc aggtggggga cagcctcctg gagaccagct
     3961 gcgggtcccc ccattatgcg tgtccagagg tgattaaggg ggaaaaatat gatggccgcc
     4021 gggcagacat gtggagctgt ggagtcatcc tcttcgccct gctcgtgggg gctctgccct
     4081 ttgatgacga caacctccgc cagctgctgg agaaggtgaa acgaggcgtc ttccacatgc
     4141 cccacttcat tcctccagat tgccagagcc tcctgagggg aatgatcgaa gtggagcccg
     4201 aaaaaaggct cagtctggag caaattcaga aacatccttg gtacctaggc gggaaacacg
     4261 agccagaccc gtgcctggag ccagcccctg gccgccgggt agccatgcgg agcctgccat
     4321 ccaacggaga gctggacccc gacgtcctag agagcatggc atcactgggc tgcttcaggg
     4381 accgcgagag gctgcatcgc gagctgcgca gtgaggagga gaaccaagaa aagatgatat
     4441 attatctgct tttggatcgg aaggagcggt atcccagctg tgaggaccag gacctgcctc
     4501 cccggaatga tgttgacccc ccccggaagc gtgtggattc tcccatgctg agccgtcacg
     4561 ggaagcggcg accagagcgg aagtccatgg aagtcctgag catcaccgat gccgggggtg
     4621 gtggctcccc tgtacccacc cgacgggcct tggagatggc ccagcacagc cagagatccc
     4681 gtagcgtcag tggagcctcc acgggtctgt cctccagccc tctaagcagc ccaaggagtc
     4741 cggtcttttc cttttcaccg gagccggggg ctggagatga ggctcgaggc gggggctccc
     4801 cgacttccaa aacgcagacg ctgccttctc ggggccccag gggtgggggc gccggggagc
     4861 agcccccgcc ccccagtgcc cgctccacac ccctgcccgg ccccccaggc tccccgcgct
     4921 cctctggcgg gacccccttg cactcgcctc tgcacacgcc ccgggccagt cccaccggga
     4981 ccccggggac aacaccaccc cccagccccg gcggtggcgt cgggggagcc gcctggagga
     5041 gtcgtctcaa ctccatccgc aacagcttcc tgggctcccc tcgctttcac cggcgcaaga
     5101 tgcaggtccc taccgctgag gagatgtcca gcttgacgcc agagtcctcc ccggagctgg
     5161 caaaacgctc ctggttcggg aacttcatct ccttggacaa agaagaacaa atattcctcg
     5221 tgctaaagga caaacctctc agcagcatca aagcagacat cgtccatgcc tttctgtcga
     5281 tccccagcct gagtcacagt gtgctgtcac agaccagctt cagggccgag tacaaggcca
     5341 gtggcggccc ctccgtcttc caaaagcccg tccgcttcca ggtggacatc agctcctctg
     5401 agggtccaga gccctccccg cgacgggacg gcagcggagg tggtggcatc tactccgtca
     5461 ccttcactct catctcgggt cccagccgtc ggttcaagcg agtggtggag accatccagg
     5521 cacagctcct gagcactcat gaccagccct ccgtgcaggc cctggcagac gagaagaacg
     5581 gggcccagac ccggcctgct ggtgccccac cccgaagcct gcagccccca cccggccgcc
     5641 cagacccaga gctgagcagc tctccccgcc gaggcccccc caaggacaag aagctcctgg
     5701 ccaccaacgg gacccctctg cccaacccag ctttcttgta caaagtgggc aagcctatcc
     5761 ctaaccctct cctcggtctc gattctacgt aataacactg gatataccac cgttgatata
     5821 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     5881 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     5941 aagttttatc cggcctttat tcacattctt gcccgcctgt aatcaacttt attatacata
     6001 gttgatatcc agcacagtgg cggccgctcg acaatcaacc tctggattac aaaatttgtg
     6061 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6121 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6181 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6241 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6301 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6361 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6421 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6481 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6541 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6601 ccctttgggc cgcctccccg cctggaattc taccgggtag gggaggcgct tttcccaagg
     6661 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     6721 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     6781 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     6841 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     6901 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     6961 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     7021 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     7081 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     7141 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     7201 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     7261 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     7321 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     7381 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     7441 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     7501 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     7561 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     7621 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     7681 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     7741 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac
     7801 cccatgcatc tcgagggccc ggtaccttta agaccaatga cttacaaggc agctgtagat
     7861 cttagccact ttttaaaaga aaagggggga ctggaagggc tagctcactc ccaacgaaga
     7921 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     7981 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8041 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8101 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8161 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     8221 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8281 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     8341 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     8401 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     8461 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     8521 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8581 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8641 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     8701 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     8761 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     8821 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     8881 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     8941 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     9001 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     9061 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     9121 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     9181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     9541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10321 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10381 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10441 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    10501 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10561 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10621 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10681 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10741 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10801 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    10861 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    10921 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10981 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11041 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11101 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    11161 gactggaaag cgggcagtga gcgcaacgca at
//
