LOCUS       sequence-269114-       13913 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK12 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13913)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 13913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-CDK12-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..13913
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7855..7879)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7881..7922
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7929..8699
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(8705..8725)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    8754..9342
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8807..8827)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9225..9236)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9356..9855
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9391..9414)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9788..9807
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9876..10475
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9876..9895)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10372..10392
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10613..10846
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10918..11039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10955..10974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11009..11028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11079..11214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11234..11253)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11235..11253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11263..11280)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11263..11279)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11272..11294)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11421..11876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11508..11527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11718..11739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11902..12006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12007..12075,12076..12867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      13038..13626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13527..13546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13780..13797
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggcttag ccaccatgcc caattcagag agacatgggg
     3421 gcaagaagga cgggagtgga ggagcttctg gaactttgca gccgtcatcg ggaggcggca
     3481 gctctaacag cagagagcgt caccgcttgg tatcgaagca caagcggcat aagtccaaac
     3541 actccaaaga catggggttg gtgacccccg aagcagcatc cctgggcaca gttatcaaac
     3601 ctttggtgga gtatgatgat atcagctctg attccgacac cttctccgat gacatggcct
     3661 tcaaactaga ccgaagggag aacgacgaac gtcgtggatc agatcggagc gaccgcctgc
     3721 acaaacatcg tcaccaccag cacaggcgtt cccgggactt actaaaagct aaacagaccg
     3781 aaaaagaaaa aagccaagaa gtctccagca agtcgggatc gatgaaggac cggatatcgg
     3841 gaagttcaaa gcgttcgaat gaggagactg atgactatgg gaaggcgcag gtagccaaaa
     3901 gcagcagcaa ggaatccagg tcatccaagc tccacaagga gaagaccagg aaagaacggg
     3961 agctgaagtc tgggcacaaa gaccggagta aaagtcatcg aaaaagggaa acacccaaaa
     4021 gttacaaaac agtggacagc ccaaaacgga gatccaggag cccccacagg aagtggtctg
     4081 acagctccaa acaagatgat agcccctcgg gagcttctta tggccaagat tatgacctta
     4141 gtccctcacg atctcatacc tcgagcaatt atgactccta caagaaaagt cctggaagta
     4201 cctcgagaag gcagtcggtc agtccccctt acaaggagcc ttcggcctac cagtccagca
     4261 cccggtcacc gagcccctac agtaggcgac agagatctgt cagtccctat agcaggagac
     4321 ggtcgtccag ctacgaaaga agtggctctt acagcgggcg atcacccagt ccctatggtc
     4381 gaaggcggtc cagcagccct ttcctgagca agcggtctct gagtcggagt ccactcccca
     4441 gtaggaaatc catgaagtcc agaagtagaa gtcctgcata ttcaagacat tcatcttctc
     4501 atagtaaaaa gaagagatcc agttcacgca gtcgtcattc cagtatctca cctgtcaggc
     4561 ttccacttaa ttccagtctg ggagctgaac tcagtaggaa aaagaaggaa agagcagctg
     4621 ctgctgctgc agcaaagatg gatggaaagg agtccaaggg ttcacctgta tttttgccta
     4681 gaaaagagaa cagttcagta gaggctaagg attcaggttt ggagtctaaa aagttaccca
     4741 gaagtgtaaa attggaaaaa tctgccccag atactgaact ggtgaatgta acacatctaa
     4801 acacagaggt aaaaaattct tcagatacag ggaaagtaaa gttggatgag aactccgaga
     4861 agcatcttgt taaagatttg aaagcacagg gaacaagaga ctctaaaccc atagcactga
     4921 aagaggagat tgttactcca aaggagacag aaacatcaga aaaggagacc cctccacctc
     4981 ttcccacaat tgcttctccc ccaccccctc taccaactac tacccctcca cctcagacac
     5041 cccctttgcc acctttgcct ccaataccag ctcttccaca gcaaccacct ctgcctcctt
     5101 ctcagccagc atttagtcag gttcctgctt ccagtacttc aactttgccc ccttctactc
     5161 actcaaagac atctgctgtg tcctctcagg caaattctca gccccctgta caggtttctg
     5221 tgaagactca agtatctgta acagctgcta ttccacacct gaaaacttca acgttgcctc
     5281 ctttgcccct cccaccctta ttacctggag atgatgacat ggatagtcca aaagaaactc
     5341 ttccttcaaa acctgtgaag aaagagaagg aacagaggac acgtcactta ctcacagacc
     5401 ttcctctccc tccagagctc cctggtggag atctgtctcc cccagactct ccagaaccaa
     5461 aggcaatcac accacctcag caaccatata aaaagagacc aaaaatttgt tgtcctcgtt
     5521 atggagaaag aagacaaaca gaaagcgact gggggaaacg ctgtgtggac aagtttgaca
     5581 ttattgggat tattggagaa ggaacctatg gccaagtata taaagccaag gacaaagaca
     5641 caggagaact agtggctctg aagaaggtga gactagacaa tgagaaagag ggcttcccaa
     5701 tcacagccat tcgtgaaatc aaaatccttc gtcagttaat ccaccgaagt gttgttaaca
     5761 tgaaggaaat tgtcacagat aaacaagatg cactggattt caagaaggac aaaggtgcct
     5821 tttaccttgt atttgagtat atggaccatg acttaatggg actgctagaa tctggtttgg
     5881 tgcacttttc tgaggaccat atcaagtcgt tcatgaaaca gctaatggaa ggattggaat
     5941 actgtcacaa aaagaatttc ctgcatcggg atattaagtg ttctaacatt ttgctgaata
     6001 acagtgggca aatcaaacta gcagattttg gacttgctcg gctctataac tctgaagaga
     6061 gtcgccctta cacaaacaaa gtcattactt tgtggtaccg acctccagaa ctactgctag
     6121 gagaggaacg ttacacacca gccatagatg tttggagctg tggatgtatt cttggggaac
     6181 tattcacaaa gaagcctatt tttcaagcca atctggaact ggctcagcta gaactgatca
     6241 gccgactttg tggtagccct tgtccagctg tgtggcctga tgttatcaaa ctgccctact
     6301 tcaacaccat gaaaccgaag aagcaatatc gaaggcgtct acgagaagaa ttctctttca
     6361 ttccttctgc agcacttgat ttattggacc acatgctgac actagatcct agtaagcggt
     6421 gcacagctga acagacccta cagagcgact tccttaaaga tgtcgaactc agcaaaatgg
     6481 ctcctccaga cctcccccac tggcaggatt gccatgagtt gtggagtaag aaacggcgac
     6541 gtcagcgaca aagtggtgtt gtagtcgaag agccacctcc atccaaaact tctcgaaaag
     6601 aaactacctc agggacaagt actgagcctg tgaagaacag cagcccagca ccacctcagc
     6661 ctgctcctgg caaggtggag tctggggctg gggatgcaat aggccttgct gacatcacac
     6721 aacagctgaa tcaaagtgaa ttggcagtgt tattaaacct gctgcagagc caaaccgacc
     6781 tgagcatccc tcaaatggca cagctgctta acatccactc caacccagag atgcagcagc
     6841 agctggaagc cctgaaccaa tccatcagtg ccctgacgga agctacttcc cagcagcagg
     6901 actcagagac catggcccca gaggagtctt tgaaggaagc accctctgcc ccagtgatcc
     6961 tgccttcagc agaacagatg acccttgaag cttcaagcac accagctgac atgcagaata
     7021 tattggcagt tctcttgagt cagctgatga aaacccaaga gccagcaggc agtctggagg
     7081 aaaacaacag tgacaagaac agtgggccac aggggccccg aagaactccc acaatgccac
     7141 aggaggaggc agcagagaag aggccccctg agccccccgg acctccaccg ccgccacctc
     7201 caccccctct ggttgaaggc gatctttcca gcgcccccca ggagttgaac ccagccgtga
     7261 cagccgcctt gctgcaactt ttatcccagc ctgaagcaga gcctcctggc cacctgccac
     7321 atgagcacca ggccttgaga ccaatggagt actccacccg accccgtcca aacaggactt
     7381 atggaaacac tgatgggcct gaaacagggt tcagtgccat tgacactgat gaacgaaact
     7441 ctggtccagc cttgacagaa tccttggtcc agaccctggt gaagaacagg accttctcag
     7501 gctctctgag ccaccttggg gagtccagca gttaccaggg cacagggtca gtgcagtttc
     7561 caggggacca ggacctccgt tttgccaggg tccccttagc gttacacccg gtggtcgggc
     7621 aaccattcct gaaggctgag ggaagcagca attctgtggt acatgcagag accaaattgc
     7681 aaaactatgg ggagctgggg ccaggaacca ctggggccag cagctcagga gcaggccttc
     7741 actggggggg cccaactcag tcttctgctt atggaaaact ctatcggggg cctacaagag
     7801 tcccaccaag agggggaaga gggagaggag ttccttactc aggctcgagc ggcaacccag
     7861 ctttcttgta caaagtgggc ggcaagccca tccccaaccc cctgctgggc ctggacagca
     7921 ccgctagcat cccgctgctg aacgctaaac agattctggg acagctggac ggcgggagcg
     7981 tggcagtcct gcctgtggtc gactccacca atcagtacct gctggatcga atcggcgagc
     8041 tgaagagtgg ggatgcttgc attgcagaat atcagcaggc agggagagga agcagaggga
     8101 ggaaatggtt ctctcctttt ggagctaacc tgtacctgag tatgttttgg cgcctgaagc
     8161 ggggaccagc agcaatcggc ctgggcccgg tcatcggaat tgtcatggca gaagcgctgc
     8221 gaaagctggg agcagacaag gtgcgagtca aatggcccaa tgacctgtat ctgcaggata
     8281 gaaagctggc aggcatcctg gtggagctgg ccggaataac aggcgatgct gcacagatcg
     8341 tcattggcgc cgggattaac gtggctatga ggcgcgtgga ggaaagcgtg gtcaatcagg
     8401 gctggatcac actgcaggaa gcagggatta acctggacag gaatactctg gccgctatgc
     8461 tgatccgaga gctgcgggca gccctggaac tgttcgagca ggaaggcctg gctccatatc
     8521 tgtcacggtg ggagaagctg gataacttca tcaatagacc cgtgaagctg atcattgggg
     8581 acaaagagat tttcgggatt agccggggga ttgataaaca gggagccctg ctgctggaac
     8641 aggacggagt tatcaaaccc tggatgggcg gagaaatcag tctgcggtct gccgaaaagt
     8701 aagccaactt tattatacat agttgatatc cagcacagtg gcggccgctc gacaatcaac
     8761 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     8821 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     8881 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     8941 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     9001 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     9061 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     9121 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     9181 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     9241 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     9301 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt ctaccgggta
     9361 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     9421 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     9481 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     9541 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     9601 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     9661 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     9721 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     9781 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     9841 tctccgggcc tttcgacctg cagcccaagc ttaccatgac cgagtacaag cccacggtgc
     9901 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     9961 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
    10021 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
    10081 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
    10141 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
    10201 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
    10261 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
    10321 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
    10381 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
    10441 gcacctggtg catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc
    10501 cgaccgaaag gagcgcacga ccccatgcat ctcgagggcc cggtaccttt aagaccaatg
    10561 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
    10621 ctagctcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
    10681 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
    10741 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
    10801 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
    10861 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
    10921 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10981 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    11041 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
    11101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    11161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
    11221 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
    11281 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    11341 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    11401 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
    11461 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
    11521 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
    11581 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
    11641 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
    11701 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
    11761 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
    11821 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
    11881 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
    11941 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
    12001 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
    12061 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
    12121 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
    12181 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
    12241 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
    12301 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
    12361 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
    12421 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
    12481 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
    12541 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
    12601 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
    12661 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
    12721 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
    12781 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
    12841 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
    12901 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
    12961 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
    13021 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
    13081 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
    13141 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
    13201 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
    13261 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
    13321 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    13381 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    13441 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    13501 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    13561 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    13621 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
    13681 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
    13741 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
    13801 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
    13861 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aat
//
