LOCUS       sequence-269115-       10981 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of BRSK2 (human) tagged with N-terminal V5
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10981)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10981)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-V5-BRSK2_IDG-K
FEATURES             Location/Qualifiers
     source          1..10981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2153..3364
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3201..3220
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3368..3388)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     LTR             3392..3660
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3670..3711
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    3709..3733
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5768..5792)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5822..6410
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5875..5895)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6293..6304)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6424..6923
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6459..6482)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6856..6875
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6944..7543
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6944..6963)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7440..7460
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7681..7914
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7986..8107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8023..8042)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8077..8096
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8147..8282
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8302..8321)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8303..8321)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8331..8348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8331..8347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8340..8362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8489..8944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8576..8595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8786..8807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8970..9074
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9075..9143,9144..9935)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9293..9312)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10106..10694
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10595..10614
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10848..10865
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3361 agacggccaa cttttgtata caaagtggcg aggggctcgc atctctcctt cacgcgcccg
     3421 ccgccctacc tgaggccgcc atccacgccg gttgagtcgc gttctgccgc ctcccgcctg
     3481 tggtgcctcc tgaactgcgt ccgccgtcta ggtaagttta aagctcaggt cgagaccggg
     3541 cctttgtccg gcgctccctt ggagcctacc tagactcagc cggctctcca cgctttgcct
     3601 gaccctgctt gctcaactct acgtctttgt ttcgttttct gttctgcgcc gttacagatc
     3661 ggcaccatgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac aactttgtac
     3721 aaaaaagcag aagggccgtc aaggcccacc atgacatcga cggggaagga cggcggcgcg
     3781 cagcacgcgc agtatgttgg gccctaccgg ctggagaaga cgctgggcaa ggggcagaca
     3841 ggtctggtga agctgggggt tcactgcgtc acctgccaga aggtggccat caagatcgtc
     3901 aaccgtgaga agctcagcga gtcggtgctg atgaaggtgg agcgggagat cgcgatcctg
     3961 aagctcattg agcaccccca cgtcctaaag ctgcacgacg tttatgaaaa caaaaaatat
     4021 ttgtacctgg tgctagaaca cgtgtcaggt ggtgagctct tcgactacct ggtgaagaag
     4081 gggaggctga cgcctaagga ggctcggaag ttcttccggc agatcatctc tgcgctggac
     4141 ttctgccaca gccactccat atgccacagg gatctgaaac ctgaaaacct cctgctggac
     4201 gagaagaaca acatccgcat cgcagacttt ggcatggcgt ccctgcaggt tggcgacagc
     4261 ctgttggaga ccagctgtgg gtccccccac tacgcctgcc ccgaggtgat ccggggggag
     4321 aagtatgacg gccggaaggc ggacgtgtgg agctgcggcg tcatcctgtt cgccttgctg
     4381 gtgggggctc tgcccttcga cgatgacaac ttgcgacagc tgctggagaa ggtgaagcgg
     4441 ggcgtgttcc acatgccgca ctttatcccg cccgactgcc agagtctgct acggggcatg
     4501 atcgaggtgg acgccgcacg ccgcctcacg ctagagcaca ttcagaaaca catatggtat
     4561 atagggggca agaatgagcc cgaaccagag cagcccattc ctcgcaaggt gcagatccgc
     4621 tcgctgccca gcctggagga catcgacccc gacgtgctgg acagcatgca ctcactgggc
     4681 tgcttccgag accgcaacaa gctgctgcag gacctgctgt ccgaggagga gaaccaggag
     4741 aagatgattt acttcctcct cctggaccgg aaagaaaggt acccgagcca ggaggatgag
     4801 gacctgcccc cccggaacga gatagaccct ccccggaagc gtgtggactc cccgatgctg
     4861 aaccggcacg gcaagcggcg gccagaacgc aaatccatgg aggtgctcag cgtgacggac
     4921 ggcggctccc cggtgcctgc gcggcgggcc attgagatgg cccagcacgg ccagaggtct
     4981 cggtccatca gcggtgcctc ctcaggcctt tccaccagcc cactcagcag cccccgggtg
     5041 acccctcacc cctcaccaag gggcagtccc ctccccaccc ccaaggggac acctgtccac
     5101 acgccaaagg agagcccggc tggcacgccc aaccccacgc ccccgtccag ccccagcgtc
     5161 ggaggggtgc cctggagggc gcggctcaac tccatcaaga acagctttct gggctcaccc
     5221 cgcttccacc gccggaaact gcaagttccg acgccggagg agatgtccaa cctgacacca
     5281 gagtcgtccc cagagctggc gaagaagtcc tggtttggga acttcatcag cctggagaag
     5341 gaggagcaga tcttcgtggt catcaaagac aaacctctga gctccatcaa ggctgacatc
     5401 gtgcacgcct tcctgtcgat tcccagtctc agccacagcg tcatctccca aacgagcttc
     5461 cgggccgagt acaaggccac gggggggcca gccgtgttcc agaagccggt caagttccag
     5521 gttgatatca cctacacgga gggtggggag gcgcagaagg agaacggcat ctactccgtc
     5581 accttcaccc tgctctcagg ccccagccgt cgcttcaaga gggtggtgga gaccatccag
     5641 gcccagctgc tgagcacaca cgacccgcct gcggcccagc acttgtcaga caccactaac
     5701 tgtatggaaa tgatgacggg gcggctttcc aaatgtggaa ttatcccgaa aagttgaggc
     5761 ctcatgggcc cagctttctt gtacaaagtt gtgatatcca gcacagtggc ggccgctcga
     5821 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5881 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5941 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6001 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6061 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6121 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6181 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6241 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6301 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6361 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattct
     6421 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     6481 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     6541 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     6601 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     6661 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     6721 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     6781 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     6841 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     6901 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     6961 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     7021 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     7081 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     7141 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     7201 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     7261 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     7321 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     7381 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     7441 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     7501 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc
     7561 gcagcgcccg accgaaagga gcgcacgacc ccatgcatct cgagggcccg gtacctttaa
     7621 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7681 tggaagggct agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7741 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7801 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7861 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7921 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7981 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8041 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8101 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8161 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8221 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8281 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8341 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8401 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     8461 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     8521 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     8581 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8641 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8701 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8761 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8821 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8881 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8941 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     9481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     9541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     9601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     9661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     9721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    10501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    10561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    10621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    10681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    10741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10861 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10981 t
//
