LOCUS       sequence-269238-       19301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19301)
  AUTHORS   Nam HS, Capecchi MR
  TITLE     Lrig1 expression prospectively identifies stem cells in the 
            ventricular-subventricular zone that are neurogenic throughout adult
            life.
  JOURNAL   Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.
  PUBMED    32183906
REFERENCE   2  (bases 1 to 19301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Dev."; date: "2020-03-17"; volume: "15(1):3. doi"; pages: " 
            10.1186/s13064-020-00139-5"
COMMENT     Sequence Label: pB6-Lrig1-T2A-tdTomato-FNF-TK
FEATURES             Location/Qualifiers
     source          1..19301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(12..36)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        281..343
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
     5'UTR           365..404
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             422..1552
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(1550..1569)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1594..1642
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(1946..1963)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1946..1962)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1955..1977)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2171..2190)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2290..2312
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2350..2368)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2436..2540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2541..2609,2610..3401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2759..2778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3566..4111
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     4012..4031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4228..4245
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4395..4414
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4395..4413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4656..4680
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10612..10665
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10675..12105
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    complement(12138..12171)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    12226..12450
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     12439..12456
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(12488..13291)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12599..12618)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13209..13228
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(13310..13357)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(13369..13868)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(13417..13436)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    complement(13932..13965)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             17628..17639
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gagatatcta gacccagctt tcttgtacaa agtggttgat tcgaggctgc taacaaatcg
       61 agcagtgtgg ttttcaagag gaagcaaaaa gcctctccac ccaggcctgg aatgtttcca
      121 cccaatgtcg agcagtgtgg ttttgcaaga ggaagcaaaa agcctctcca cccaggcctg
      181 gaatgtttcc acccaatgtc gagcaaaccc cgcccagcgt cttgtcattg gcgaattcga
      241 acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc
      301 gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc
      361 cgcagatctt ggtggcgtga aactcccgca cctcttcggc cagcgccttg tagaagcgcg
      421 tatggcttcg taccccggcc atcaacacgc gtctgcgttc gaccaggctg cgcgttctcg
      481 cggccatagc aaccgacgta cggcgttgcg ccctcgccgg cagcaagaag ccacggaagt
      541 ccgcccggag cagaaaatgc ccacgctact gcgggtttat atagacggtc cccacgggat
      601 ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta
      661 cgtacccgag ccgatgactt actggcgggt gctgggggct tccgagacaa tcgcgaacat
      721 ctacaccaca caacaccgcc tcgaccaggg tgagatatcg gccggggacg cggcggtggt
      781 aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg ccgttctggc
      841 tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc tcaccctcat
      901 cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcggt accttatggg
      961 cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga ccttgcccgg
     1021 caccaacatc gtgcttgggg cccttccgga ggacagacac atcgaccgcc tggccaaacg
     1081 ccagcgcccc ggcgagcggc tggacctggc tatgctggct gcgattcgcc gcgtttacgg
     1141 gctacttgcc aatacggtgc ggtatctgca gtgcggcggg tcgtggcggg aggactgggg
     1201 acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca acgcgggccc
     1261 acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt tgctggcccc
     1321 caacggcgac ctgtataacg tgtttgcctg ggccttggac gtcttggcca aacgcctccg
     1381 ttccatgcac gtctttatcc tggattacga ccaatcgccc gccggctgcc gggacgccct
     1441 gctgcaactt acctccggga tggtccagac ccacgtcacc acccccggct ccataccgac
     1501 gatatgcgac ctggcgcgca cgtttgcccg ggagatgggg gaggctaact gaaacacgga
     1561 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     1621 acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     1681 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     1741 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     1801 gccatagcca ctggccccgt gggttaggga cggggtcccc catggggaat ggtttatggt
     1861 tcgtgggggt tattattttg ggcgttgcgt ggggtcaggt ccacgaccca agcttggctg
     1921 caggtcgagc tcgcgaaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1981 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2041 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2101 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     2161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     2221 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     2281 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     2341 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     2401 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     2461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2521 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2581 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2641 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2701 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2761 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     2821 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2881 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2941 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3001 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3061 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     3121 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3181 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3241 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3301 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3361 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3421 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3481 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3541 cagacccctt aataagatga tcttcttgag atcgttttgg tctgcgcgta atctcttgct
     3601 ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga gctaccaact
     3661 ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc ctttcagttt
     3721 agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc agtggctgct
     3781 gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt accggataag
     3841 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     3901 tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
     3961 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag ggggaaacgc
     4021 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg
     4081 atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc
     4141 ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc
     4201 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc
     4261 ctgtatcaca tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca
     4321 cttcactgac accctcatca gtgccaacat agtaagccag tatacactcc gctagcaacc
     4381 tggcttatcg aaattaatac gactcactat agggagaccg gcagatccgg cgcgcctgca
     4441 ggcgcgccac tagttaatta tttaaatcga tgcgacgcta gcggccgcgt ttgcttttgt
     4501 ttcgtgttgg tcaccacggc cgagtttcct cgggaccccg ggcagctgct ttacatccca
     4561 aagacctatc tgctcggccg gcctcggaac gcgagcctgc ccggaagatc cccgggtacc
     4621 gagctcgaat tcatcgtcac catcacctcg aatcaacaag tttgtacaaa aaagcaggct
     4681 ttaaaggaac caattcagtc gagaccatgg ctgtggtggg caaggacatc cgtttcacat
     4741 gctccgcagc cagcagcagc agctcaccaa tgaccttcgc ctggaagaag gacaatgagg
     4801 tcctggccaa tgcagacatg gagaactttg cccacgtccg tgcacaggac ggcgaagtga
     4861 tggagtatac cactatcctg cacctccgtc acgtcacctt tgggcacgag ggccgctacc
     4921 agtgtatcat cacaaaccac tttggctcca catactccca caaagccagg ctcactgtga
     4981 atggtaagga catggcatct cgtgcttctc aagcacagag tcccacagac tacactgctg
     5041 attgtcctct ctgacactgg tgtgaagaga cacgagccgt ggggtgcatc tctttgtacc
     5101 agtccttggg tttgtcccag accaagttta acagattgaa ccatcgtttt ttgtttgggg
     5161 tgggagattt ttaaagtcca gttatgttaa gtgacattgt ggagttaagc tttgtatttt
     5221 tatgttcctt gtcttccata agccaagaga tgcggtttct agattccaag catccctagg
     5281 ttctaaggaa gcttgtgacc ttagagtaga tgttataact gggagcaagg ccgtttggga
     5341 caagtacaag ccccacttcc tctagaacaa tggttcttaa ccttcctaat gcttgacccc
     5401 ttaatatagt ccctcatgct gtgctgaccc ccaaccataa aactactttc tttgctattt
     5461 cataactgta acttcgcaac tgttatgaat cacagtgtaa atatttttga acatagaggt
     5521 ttgccaaagg ggttcatgat ccacagggtg acccattgtt ccgagccgct actcttcagg
     5581 ctgtggagag caggtgtggg ttgagcccct ggctccgagg tccagtgtgc agggaagttg
     5641 tgacaagggt ctcttactca ggagccagcc cctggccatc agggtggctt tcttctggac
     5701 tactgatgga tgtggtatgt tgagggatga ggacagcatc agtagctaac tcttccacac
     5761 taggctaagc tggcttgtgg gtggcctgaa gcactgcctc catgctctct gctattgtac
     5821 aacaccacaa agcactctac cacagcgagg ctggcctaag agaggccaga gtgtttcttg
     5881 gagaccaagt agctgtagac tctccaccac agtagctcag acatcaagtc tggatactgt
     5941 ccactgtggc agcattgata ctaaagctac agaagcgaac gaactacata tttttgcctg
     6001 ggtgtggctg gtagtgtagc ccccatagac cccacccaga gtgggtttcc tcaagagagg
     6061 tgagccagct cactggttcc tcctggtgac agcatccagc tatcccctaa tagcaaatgt
     6121 tttgttgccc agctctctcc agatagctaa ctaaagcagg tcagcaccag acttttactt
     6181 ctggaggatt ccctacatag taactactgt ggagcttcgg gaggcaaaac gctccatcag
     6241 aagccttccg agagtgctta gcaccaggct atagcaatag gaaggcgtta agggtaatgg
     6301 gtgcagtatt gtgtctgtga cggggagagg ctgggcaccc cttgggtggg atggagtaac
     6361 aatgaactga ggcctgggca cagtgggatc tgtttactat gagtttcctt ttgtatgcag
     6421 ccattccaag tagctttacc tatcattttt ttccagtgtt gccatcattc actaaaatac
     6481 cccatgacat tgccatccgg actggcacca cagcccgcct cgagtgtgct gccacgggcc
     6541 accctaaccc tcagattgcc tggcagaagg atggaggcac cgatttcccg gcagctcgtg
     6601 agcgacgcat gcatgttatg ccagacgatg atgtgttctt catcactgat gtgaaaatag
     6661 acgacatggg ggtctacagc tgcactgccc agaactcggc aggctcggtt tcagccaacg
     6721 ctaccctcac agtcttaggt tcgctacgac agctctcgcg cacgtgtggc agggtcgggt
     6781 ggggattcaa ctcaagtatt agagaacctg ctttctggag tccctgtggt agactctgtc
     6841 tagttaattt gaattttggg gctgctttta agcgagtagt ttcagctcat aaaagacaat
     6901 gagctatatg ttagtctgcg tgagaattct gtgtgtgtgt gtgtgtgcac tcacacaggt
     6961 gggctatgat cttaggagta gtgacaaagg tctataacag gacagtttgg gatagggtag
     7021 ggctttagtt ctttgctctg ttgagctgtt cctagaatgg cagtgattta attacattag
     7081 gctttctgga aagcttggtg ctccaataaa agatctcaga tagcatttca ctacaacatg
     7141 ggattgtctt ctgtctggaa aacatgtata cctccagcaa gcgagaccca tagaataaat
     7201 gtgcaatttc cactgccttc ctgcagaaac tccatccttg gcagtgcctc tggaagaccg
     7261 tgtggtaact gtgggagaaa cagtggcctt ccagtgcaaa gcaaccggga gccccacacc
     7321 acgcatcacc tggcttaagg gaggtcgccc attgagcctc acagagcgcc accatttcac
     7381 tccaggcaac cagctgctgg ttgttcagaa tgtgatgata gacgatgcag ggcggtatac
     7441 ctgtgagatg tctaatcccc tgggcactga gcgagcacat agccagctga gcattttacc
     7501 tacccctggc tgccggaagg atgggaccac cgtaggcatc ttcaccattg ctgtggtgtg
     7561 cagtattgtc ctgacctcgc tggtgtgggt gtgcatcatc taccagacca ggaagaagag
     7621 cgaggagtac agcgtcacta acacaggtca gcagcacagc ggacttgggg ggctgggggc
     7681 agatggggtg acactgccgc agtccgaggt aaacgatgca ggcctggggg cttgcctaga
     7741 acctgaaagg tctctagaac atttaggaat atggactctg cctgtagagt tgggactttg
     7801 gtccatgtcc tccccccccc ccaacagcaa gctagctctg aaggacagaa cagaaaatgg
     7861 gaatactctc acatgcaagg actcgctggt ctctgcagtt tctggtctct ttggcagctc
     7921 agaggcccta gggccacgcc tgttatgaac cggattgttc ctaggctctc agtcttggtt
     7981 aggccttcct ttccttgttc ctcatggagc caggcgtctg actgccgtga tgtttacacc
     8041 agggtgtaaa ggaggttaat gagcggctgc cgagcctctg cacagctttc tgtgtgtgag
     8101 ccactgtcgt gtgaccttgt gatagtgcgt agttatctgt atttattgtt gtggcagccc
     8161 tgttatttta ttgtcttggc tccttgcaga tgaaaccatt gtgcctccag atgttccaag
     8221 ctacctctcc tctcagggaa ccctttctga ccggcaggaa actgtggtca ggactgaggg
     8281 tggtcatcag gccaatgggc acattgaaag caatggtaag ttcggaacag tgagccagct
     8341 gccctcgtcc tggatgaagt ctggcactgg gtcacatttg gtgaaccagg cctttaattg
     8401 ataagatagt ttgctttgga tctggtgtgt gcaagttcta tacttttaac gttagatcct
     8461 ccggaaagtc cctgcactgg gcttagcact ctgtcttctg gtcagaagga ttatatgata
     8521 ggattgccag aaagaatata tgcaaaggac attccaggcc cccaaatcag cccatagctt
     8581 ttacagtact gtagcacttt acatgggcta agtataagtc aaccaaccac agactgaaaa
     8641 tatttagcaa agaaaaactg aatctatatg gacattttac gtcgttacta ggtactatga
     8701 gtgaagatga cttaaggtct gtcagagaag gtgtacagtt tatgtgcaaa cagcatttca
     8761 cagaaaagga acttggcatc cttggatttg ggcatctcag aggagcatag gaaccaatcc
     8821 ctgggtggat gccaaggtgc tgggaaaagc agtggtctgg tggccatcag gctgcgtgat
     8881 gtaagcagct tctaagtggt aagggctctg tttcaggtgt gtgtctgaga gacccgagcc
     8941 tcttcccaga ggtcgacatt catagcacta catgcaggca gcccaagctg tgtgttggat
     9001 acactagaga gccctggaag gtgacggaga aagccgacag gacagctgcc ccacatacaa
     9061 caggtaggag acacttaagt caggggacgc ggttgctttt ggccctgaag ctggcttgac
     9121 tgagtaaact agagtagcgg cagatcactg gccgcacgct gtctctgagt tatgtttaga
     9181 atacaaacag gattttgtgt gattctagag tcctgtgtcg cttaggtaag tcagtctatg
     9241 atgtggtgag ctgaaggctg agttttctta acatggctga gttttcttaa cataaggaac
     9301 ttacaagaca aggcctgccc ttgcgccagg actgttgtcc cgacaaggtt tcacttcgtg
     9361 gatcaggctg tcctcagcct cctgagatgc tgtggctggg ctaccgtgcc cgtcccttag
     9421 agtgcgcctt cctcatgtca ctcagctcat gtcctcagtc actctttgtc ctgtgtaatg
     9481 aacagggatt ctctctcaaa cccagggttc actgatccaa ttttctagcc cgctagcctg
     9541 ctccggggtt tcctgcctct gcgttagctg ggccaccaca cctgcctggc atttccagga
     9601 gtactgggga cctgaactcg ggtccttatg ctcagcaagc gctttcccta ctgagccctc
     9661 cctctccgca gccctgctgg cgggactctt taaggcctac tgattaagat tccttactgt
     9721 atgatgccta atgcaaaccg ttcctcttcc tctccccacc ctcattttcc tgtgattaga
     9781 gtgtgccttc gaaattaatt ttacacgtat acttaggcac acaagactaa gacatggttg
     9841 gtctagctcc tctccacata cacattccag ggggtgggga ggggagagac agggtggtta
     9901 tgaaggcagt ggtaagctgg actcatcttc caatagtgcc tctgagaggg aatggagact
     9961 cagttgacct ctggttctct tcttccctca gcacacagtg gctctgctgt atgtagtgac
    10021 tgcagtactg acacagccta ccatccccag cctgtgccca gagacagtgg gcagccaggc
    10081 acagcgagca gccaagagct caggcagcat gaccgggaat attctccaca ccatccttac
    10141 agtgggactg cggatgggtc tcataccctc tctggggggt ccctctatcc aagcaaccat
    10201 gacagaatac tgccatcctt gaagaacaag gcggcgtctg cagatgggaa cggtgtgtat
    10261 tgtgttagag gaacctcggt ggctgctggg agccctttgg ttacggtgca taggctgagc
    10321 tgcctctttt tttttttttt tttttaaaca ggagattcct cttggacttt agcaaagtta
    10381 catgaagcag actgcataga cctaaagcct tctcctacgt tagcttcagg cagcccagag
    10441 ctcatggaag acgccatatc tactgaagcc cagcacttgc ttgtttccaa tggccacctc
    10501 cccaaggcct gtgactccag ccctgaatct gtgccactga aagggcagat cactgggaaa
    10561 cggaggggac cactgctatt ggcaccgaga agcggtttaa actctggttc cgagggcaga
    10621 ggaagtctgt taacatgcgg tgacgtcgag gagaatcctg gcccatctgg ctccatggtg
    10681 agcaagggcg aggaggtcat caaagagttc atgcgcttca aggtgcgcat ggagggctcc
    10741 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
    10801 cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg ggacatcctg
    10861 tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc accccgccga catccccgat
    10921 tacaagaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
    10981 ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg acggcacgct gatctacaag
    11041 gtgaagatgc gcggcaccaa cttccccccc gacggccccg taatgcagaa gaagaccatg
    11101 ggctgggagg cctccaccga gcgcctgtac ccccgcgacg gcgtgctgaa gggcgagatc
    11161 caccaggccc tgaagctgaa ggacggcggc cactacctgg tggagttcaa gaccatctac
    11221 atggccaaga agcccgtgca actgcccggc tactactacg tggacaccaa gctggacatc
    11281 acctcccaca acgaggacta caccatcgtg gaacagtacg agcgctccga gggccgccac
    11341 cacctgttcc tggggcatgg caccggcagc accggcagcg gcagctccgg caccgcctcc
    11401 tccgaggaca acaacatggc cgtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
    11461 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
    11521 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
    11581 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
    11641 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
    11701 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
    11761 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
    11821 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
    11881 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
    11941 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
    12001 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
    12061 cgccaccacc tgttcctgta cggcatggac gagctgtaca agtaaggcgc gccggatcca
    12121 ttatgtacct gactgatgaa gttcctatac tttctagaga ataggaactt cgaagggttc
    12181 cgcaagctct agtcgagccc cagctggttc tttccgcctc agaagccata gagcccaccg
    12241 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
    12301 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
    12361 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
    12421 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgagct ctagagaatt
    12481 gatcccctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
    12541 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
    12601 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
    12661 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
    12721 gggtcacgac gagatcatcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
    12781 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
    12841 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
    12901 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
    12961 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
    13021 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
    13081 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa
    13141 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
    13201 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
    13261 gcaatccatc ttgttcaatg gccgatccca tggtttagtt cctcaccttg tcgtattata
    13321 ctatgccgat atactatgcc gatgattaat tgtcaacagg ctgcaggtcg aaaggcccgg
    13381 agatgaggaa gaggagaaca gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg
    13441 gcctccggag gaccttcggg cgcccgcccc gcccctgagc ccgcccctga gcccgccccc
    13501 ggacccaccc cttcccagcc tctgagccca gaaagcgaag gagcaaagct gctattggcc
    13561 gctgccccaa aggcctaccc gcttccattg ctcagcggtg ctgtccatct gcacgagact
    13621 agtgagacgt gctacttcca tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg
    13681 gaacttcctg actaggggag gagtagaagg tggcgcgaag gggccaccaa agaacggagc
    13741 cggttggcgc ctaccggtgg atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg
    13801 ccaagtgccc agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc
    13861 ccctacccgg tagaatttcg acgacctgca gccaagctag cttggctgga cgtaaactcc
    13921 tcttcagacc tgaagttcct atactttcta gagaatagga acttcggaat tcgcttcatc
    13981 tacctcagct cttttaaagc agtcgctaca ggaaagaggt aggagaggcc gtggaaagct
    14041 tgtgtccaga cgtcactccg gacagtccga cttccgtgtg gaatgtcagt cagtcggcta
    14101 gaggagtggc atctggagct cagaaacgtg agagactatt tgtgtacaaa aggcaggaaa
    14161 aagtatttga taccactgta cataagagtt ttcagagatt tcatatatat tatatatctt
    14221 ttacagagac tattttaatc cttagcgcat gggtcccgct tttggtgata ttttcacatt
    14281 aggttgctgt ctaattttgg agagggtcag gaatcgttct ggtgccttaa tgcacagctg
    14341 gaattcaggt aaacacatca gctgttgctg gcggttggaa aggagggcat cttggctgtg
    14401 tggcacttag tggtcagatt cgagtgcact ggccagacct atgcaggcgt ggtgttggca
    14461 gcctcactgt agagcagggt gcacgggtct gtagttgagg acgagattga gagctgttaa
    14521 tccaccaaat gcaatggctc agaacaatta agcactgcct ttcgtctttc tttgtaactc
    14581 acttggtaaa agcaaatgtc ttgtctcctt cagtggttct ttgaagctta gtgaggcgga
    14641 ggctgccagt gttggtacct gtggattttc caatagtgag ggttagctct gcctccaggg
    14701 cccgctaagt tctgctgagc aggcctgtcc acgctgttcc ttaccggtga gactgggctt
    14761 ggactgtaca agacttgagt cctagacctg ggtgtatttg cactttgggg ccctgttgct
    14821 agccatcttg tgagtgccaa tgtataatgc agtaaagact acatggaaac acaaactcag
    14881 tccttaaaac cccaggagtt gaggggaagt gttggcacag tgatggcctg tgtcaccgtg
    14941 tagtgtcata ggccatcact gtgccagagc aagcacgctg agaaggctag tctccatagt
    15001 tgatggttaa tgttacttcc acaaaatatg tgaatttgct gcttctgaga ggcaatgtga
    15061 aagaggaagt attacttttt atgtacagag ttatttattt atagaaattt tggtacagtg
    15121 tacattgaaa acatgtaaaa tattgaagtg tctaacaaat ggcataaagt gtctttaata
    15181 aaggttcatt tataaatact aagtgtgtgc ggcggtcact gctcttttat tctgctatta
    15241 atgtttctcc aaaaggagaa tacttagacg aagcagcata gactacacac tgttgcccca
    15301 gaaagacagt ggcactgcat tcctcagata gctttttttt ttccacatgg agttggttag
    15361 tcaagcctta tgctccccaa gtatgctgac cctcccacct cctgctggca tataccatga
    15421 ggacaaagga gctgctccag ctggcctcca gggacggaca gtggtgggca tgggctttgc
    15481 ttccctcaac atgttcctaa ctgaagtcac ttgggcccac ccactcactg gcgctgcagc
    15541 tgtctgctgt ctgtcacaca ctccagggac tttggggcac tttgctgtaa agtaatataa
    15601 tcatgtgttt ttataggaga aatacaaggt aattaaaaac agcatcctca ccagaaagaa
    15661 tgcatgctat gcacctgtag ctcatgcttt ttaaacaaag ctataatctt acacaatagg
    15721 cacccgccca ggggagaaac agccacacgc acggttcttg cccccttcct tttcccagtc
    15781 tggtcattca ctgcagttgt agccagtttg ctttcctggt gactccaaca gacagcagaa
    15841 agcacaggat gctaataaaa agcattctga agggcctggc cttggcctta ccgggagtat
    15901 ttattgagag gactgagacg gtggccccag tttcaccaag aaaagtttcc tggggcagcc
    15961 tgtgctttgg ccttcattaa gctgctctct gcagccatct tcagggagcc acactggagc
    16021 cccaggcaca ggaaggactg caaggacatg tagtgtctgc ttcatgggct cttcctgccc
    16081 acctctaaca gcaagcttcg cgcctggtca taggcgccca gaaagatgaa gccgcccatg
    16141 ctgattgctg ccatcctcgg gaggacacct gcgaataacc tagggacagg aaatggtcat
    16201 gttcctgact gcccgcagtt cgctcattcc atcacatcca ttgtgaaatc ccacagaaat
    16261 ttacagtgtc tttccttcct tttagaaggc tactaagtaa tattccccaa tatgcatgag
    16321 aaactggagc ttagcctagc acggccacac tagctgggaa cactcacatt aacacagtaa
    16381 ccactctccc agcagtgtgc tcgaagaagg gagtcaagga tacatctgag gtgaggctgg
    16441 taagggtgtg tggccctggg acatctgagg ggcctctagg tctcagtcag ggagacaaag
    16501 caaattctga aacagttctg ctctcctacc ctgggctcgg ttatatttgt cattggagtc
    16561 ccacaaatgc gattctacaa gaaggggatg atgaatcttt gtggactccc tttttcttgt
    16621 tcaatcgaaa taaggacgcc agagtcacat gactgtggta aaggtggagc gcctcacagt
    16681 gtcagttcga gattctcagg tgtcccatcc ccaatccagt cagaacacca cagtggtgtc
    16741 aggaagccat tctccagagg cccaggaaat ttcaaatatc catcgtgaca ttactgaagt
    16801 aatctactag tgaccttacc tggccagatg agacagcatt gtgaaaacag agtggcgcac
    16861 agctggagag aacaagacag aagccccaac cctaaaaggt taccatcccc atggtcgctg
    16921 ggctgatgaa cacagggcaa gtcctaggct gtaaattact gctttctaca gaggcagtgg
    16981 gagtgagaga gtgacagaca gaaagcctga tcttatttct actgaaaccc tgttacccag
    17041 aaaagtgctt ctatatgcag aaagacaaac ttagaagcca gaacttgggc tgcctcggtg
    17101 ggaaaaggca cttgctgtca agactgatgg ataacccaag ttcgatccct ggacaaggaa
    17161 agaaccagtt tgatgtctat gtgtgcacac tcgtggagag gctttgggta tagggcaagt
    17221 gttttattat aacaatgctc atgcctttta aagatgtggg aaagcctttg tgctaagcta
    17281 agtggaagct aatgaagaaa gctgacagga atctctgctg gatacacagc ccatgcccta
    17341 cacagccagt ggaagagatc cgctgcccct cccacctcta gcaggcagca cagaagaaag
    17401 caaggacttt acatagtggg acccctcttg ctaagctact tagggagcta gagagaatag
    17461 cttagtgggt aaagcgagca agacctgagt tcaaatgtct agaaacctcg tgaggctgcg
    17521 cgtggcagct ctgcagtaac cccagtgctc ctgtagtgac aggaagtccc ggaggctctt
    17581 gggtcagcta gcctggccca ggaagtggca aacaaggcac ctaccatata gaaggcaggc
    17641 ctggagacac tgagctgggc ttggaggtca gtctccctca gctttgccac tacccagctc
    17701 agcagaggcc agagacagat gggagcagtt acagcgtcac agatgaccaa cagactgcag
    17761 cctccttaaa gtggaggttt tgtagggggg aggggttcca cctctgctta caggcagact
    17821 tgtgagtagg gcaggttgtg ttccagggac aacaggtgac aggataagca ggagctccca
    17881 gcagcagcga gtacataaag ggcatattca agggaaaacg ttcaggggat cagccagggg
    17941 caatccagga caagtggacc tcccctctgc cctgcagctt ctgggctaac cccagtctgc
    18001 cctccagcgt ggctccctac catctcctct gagatagcat caggttcctt ggtgggaggg
    18061 gctgctggga gttatgtaga cttgataggc ttagtgtgtg tgcagggcag ggggcacaga
    18121 cagtggtggc tctatctggc actgccacag aggaagtagg ctgagccacc tgcccagaaa
    18181 gggaccagta gttttcattt gtctcttggt ccaatcactt gctcactaga ggggctggag
    18241 ttgctgtatg ctcaggggtg gccactgctg ctgggaacat gctgttcagt gctgccctgg
    18301 acaaggtgac cagagtccaa gactggagac tcagaagacg agccaaaaat ccaagctacc
    18361 tttcagcacc aaggctgggt cagaacaaac acacaccctt ggcctgtttt gctgtctttc
    18421 ctctggcact gcttagttat agtcaagtgt gtgccatgac ctggacaggg aaggccgctc
    18481 ttgcatcatc tacccagcag catgcggtgg ccacagcggg gcactgcctc atccagtgtg
    18541 tcctttcttg tcaccacttt ctcgacactg gcaaatgggc tgcagcccca caacagtata
    18601 gtcaggggcc cagctaagtc accacacaat ctggcagctg gccttcagga tgtcaaaagt
    18661 cactgtcacc agctgctgcc tcgtgagcgc agccactgct ttcctaagag gcttctcaaa
    18721 taatgcatat ggggcacaca acccatgtcc ctcctcattc catctttgtt cagtgtcaaa
    18781 ttcgtaccca aattaacaac taatatgttc ttattcctca cgcggggtca gccatgaaca
    18841 gcagactcac tccggaagct agcaccatgc ctttcctatg tctgacacat ctagagtcag
    18901 taagtcctgt ctgttagatc caatctgcac ctccgaccat cagagcagac attgagtgaa
    18961 agcacccagc caccactttg aagcatccag ctggaaaact caaacagaca tggagtttgc
    19021 tctctggcaa aagcatcacc atgtttcccg gaccatcagc tgaaatttaa aatgccaaag
    19081 ttacacaaaa atagtgatca gtgatgtacc ctggcatggg ggtgagcggg gcggctatgc
    19141 caggtaaaga ggccaggagg gcacagtgct gctgctccca atgccagcca tcccagctcc
    19201 aggtggaggc acgtccacac agcatgtcca gaccgttccg tagagaaaac ccttccgctt
    19261 ttctaacaac tcagcataga cacagctcca gcaccaccgt a
//
