LOCUS       sequence-269402-       12460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for expression of Citrine-fused MpTubulin beta 3 
            (Citrine-MpTubulin) to visualize microtubules in plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   Kimura S, Kodama Y
  TITLE     Actin-dependence of the chloroplast cold positioning response in the
            liverwort Marchantia polymorpha L.
  JOURNAL   PeerJ. 2016 Sep 28;4:e2513. eCollection 2016.
  PUBMED    27703856
REFERENCE   2  (bases 1 to 12460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2016-09-28"; volume: "4"; pages: "e2513. eCollection 2016"
COMMENT     Sequence Label: pMpGWB105-MpTubulin
FEATURES             Location/Qualifiers
     source          1..12460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     213..235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     227..244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     228..244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        759..1104
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(1125..1127,1128..1130,1131..1841)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1170..1191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1431..1450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1778..1799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1851..1875
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3217..3241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3267..3519
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3552..3568)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3552..3568)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3565..3587)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3576..3592
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3600..3606,3607..3624,3625..3630))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3645..3666
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3751..3925
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3966..4991)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(5058..5734)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5929..5953
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6474..7265
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7511..8099
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8000..8019
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8253..8270
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8285..8425
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8351..8370)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8511..8533
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8769..8963
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9029..10102)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10531..11160)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
       61 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
      121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      241 cagtgccaag cttgcatgcc tgcaggtccc cagattagcc ttttcaattt cagaaagaat
      301 gctaacccac agatggttag agaggcttac gcagcaggtc tcatcaagac gatctacccg
      361 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
      421 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
      481 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
      541 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
      601 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
      661 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
      721 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
      781 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
      841 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
      901 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
      961 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
     1021 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     1081 agttcatttc atttggagag aacacggggg actctagaat gagcatggtg agcaagggcg
     1141 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1201 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1261 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg
     1321 gctacggcct gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca
     1381 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1441 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1501 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1561 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1621 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1681 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt
     1741 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1801 ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggctgttatc acaagtttgt
     1861 acaaaaaagc aggcttcatg agagaaattc tccacatcca gggtgggcaa tgcgggaacc
     1921 agatcggagc taagttttgg gaagtgatct gtgatgagca tgggatcgac cccactggaa
     1981 cttaccgcgg agactccgat cttcagttgg agaggattaa tgtttactac aacgaggcaa
     2041 gtggtggtag gtacgttcca cgtgctgtgc tcatggatct cgaacccgga acgatggaca
     2101 gtgttcgatc tggtccgttt ggtcagatct tccgaccaga caactttgtc tttggccaaa
     2161 ctggggccgg taacaattgg gccaaggggc actacacgga gggagcggaa ttgattgact
     2221 ctgttcttga cgtcgtgcga aaggaggcgg agagctgtga ttgcctgcag ggtttccaag
     2281 tctgccactc gctgggagga ggtaccggtt caggtatggg aaccctgctg atatccaaga
     2341 tccgtgaaga atacccggac aggatgatgc tcactttctc tgtcttccca tcgccgaagg
     2401 tgtccgatac agtcgtggag ccctacaacg ctactctttc tgtccaccag cttgtagaaa
     2461 acgcggacga gtgtatggtt ctggataatg aggcattgta cgacatttgc ttccgtactc
     2521 tgaagctgac cacccctaca tttggagatc tcaatcatct gatatcagcc acaatgagtg
     2581 gtgttacttg ctgcctgagg tttcccggtc aattgaactc tgacctccga aagcttgccg
     2641 taaacctgat cccattccct cgacttcact tcttcatggt gggattcgca cccctcacgt
     2701 ctcgaggttc ccaacaatat cgtgctctga cagtgcctga actcactcag caaatgtggg
     2761 atgcgaagaa catgatgtgc gccgccgatc ctcgtcatgg acgttacctt acggcctcag
     2821 ccatgttccg cgggcgcatg agcaccaagg aagtcgacga acagatgatc aatgtccaga
     2881 ataagaactc gtcgtacttc gtcgaatgga tccccaacaa cgtgaaatcc agtgtttgcg
     2941 acattcctcc caatgggctt aaaatggcat ccactttcat cggaaactct acttctatcc
     3001 aggaaatgtt tagacgagtg agcgagcagt ttactgccat gttcagaagg aaagctttct
     3061 tgcattggta cacaggtgaa ggaatggacg agatggagtt cactgaggca gagagcaata
     3121 tgaatgatct ggtatctgaa taccagcagt atcaggatgc aagcgccgaa gatgaaggag
     3181 aatatgacga cggtgaagag cttgaagcca actaagaccc agctttcttg tacaaagtgg
     3241 tgataaccta gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa
     3301 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     3361 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     3421 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     3481 ataaattatc gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa
     3541 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3601 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3661 acattaggct gaattaggcg cgcctatttc tgaattcaac acattgcgga cgtttttaat
     3721 gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt
     3781 taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt
     3841 tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa
     3901 ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc cggtcggcat
     3961 ctactctatt tctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
     4021 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
     4081 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
     4141 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac
     4201 gcccggagtc gtggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc
     4261 tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa
     4321 tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg
     4381 acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc
     4441 caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca
     4501 gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg
     4561 tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc
     4621 gcagcgatcg catccatagc ctccgcgacc ggttgtagaa cagcgggcag ttcggtttca
     4681 ggcaggtctt gcaacgtgac accctgtgaa cggcgggaga tgcaataggt caggctctcg
     4741 ctaaactccc caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga
     4801 taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca
     4861 cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg
     4921 tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca
     4981 ggctttttca tatctcattg ccccccggat ctgcgaaagc tcgagagaga tagatttgta
     5041 gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag
     5101 gaagggtctt gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca
     5161 catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc
     5221 gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt
     5281 cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg
     5341 aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga
     5401 aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg
     5461 agagtgtcgt gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac
     5521 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
     5581 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
     5641 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
     5701 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
     5761 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
     5821 tagccaatac gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta
     5881 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     5941 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     6001 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     6061 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     6121 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     6181 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     6241 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     6301 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     6361 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     6421 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     6481 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     6541 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     6601 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     6661 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     6721 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     6781 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     6841 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     6901 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     6961 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     7021 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     7081 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     7141 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     7201 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     7261 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     7321 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     7381 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     7441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7621 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8281 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
     8341 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     8401 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     8461 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     8521 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
     8581 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
     8641 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
     8701 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
     8761 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
     8821 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
     8881 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
     8941 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
     9001 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
     9061 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
     9121 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
     9181 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
     9241 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
     9301 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
     9361 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
     9421 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
     9481 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
     9541 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
     9601 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
     9661 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
     9721 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
     9781 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
     9841 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
     9901 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
     9961 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    10021 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    10081 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
    10141 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    10201 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    10261 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
    10321 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
    10381 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
    10441 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
    10501 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
    10561 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
    10621 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
    10681 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
    10741 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
    10801 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
    10861 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
    10921 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    10981 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    11041 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    11101 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    11161 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    11221 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    11281 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    11341 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    11401 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    11461 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    11521 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    11581 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    11641 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    11701 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    11761 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    11821 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    11881 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    11941 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    12001 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    12061 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    12121 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    12181 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    12241 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    12301 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
    12361 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt
    12421 taaatatccg attattctaa taaacgctct tttctcttag
//
