LOCUS       sequence-269668-        9154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses high levels of WT SaCas9 for genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Balboa D, Weltner J, Novik Y, Eurola S, Wartiovaara K, Otonkoski T
  TITLE     Generation of a SOX2 reporter human induced pluripotent stem cell 
            line using CRISPR/SaCas9.
  JOURNAL   Stem Cell Res. 2017 Jul;22:16-19. doi: 10.1016/j.scr.2017.05.005. 
            Epub 2017 May 17.
  PUBMED    28952927
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2017-07"; volume: "22:16-19. doi"; pages: " 
            10.1016/j.scr.2017.05.005. Epub 2017 May 17"
COMMENT     Sequence Label: CAG-SaCas9-WPRE
FEATURES             Location/Qualifiers
     source          1..9154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          949..1965
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1889..1911
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1973..1992
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2030..2039
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2042..2062
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2087..5242
                     /codon_start=1
                     /product="Cas9 endonuclease from the Staphylococcus aureus 
                     Type II CRISPR/Cas system"
                     /label=SaCas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="KRNYILGLDIGITSVGYGIIDYETRDVIDAGVRLFKEANVENNEG
                     RRSKRGARRLKRRRRHRIQRVKKLLFDYNLLTDHSELSGINPYEARVKGLSQKLSEEEF
                     SAALLHLAKRRGVHNVNEVEEDTGNELSTKEQISRNSKALEEKYVAELQLERLKKDGEV
                     RGSINRFKTSDYVKEAKQLLKVQKAYHQLDQSFIDTYIDLLETRRTYYEGPGEGSPFGW
                     KDIKEWYEMLMGHCTYFPEELRSVKYAYNADLYNALNDLNNLVITRDENEKLEYYEKFQ
                     IIENVFKQKKKPTLKQIAKEILVNEEDIKGYRVTSTGKPEFTNLKVYHDIKDITARKEI
                     IENAELLDQIAKILTIYQSSEDIQEELTNLNSELTQEEIEQISNLKGYTGTHNLSLKAI
                     NLILDELWHTNDNQIAIFNRLKLVPKKVDLSQQKEIPTTLVDDFILSPVVKRSFIQSIK
                     VINAIIKKYGLPNDIIIELAREKNSKDAQKMINEMQKRNRQTNERIEEIIRTTGKENAK
                     YLIEKIKLHDMQEGKCLYSLEAIPLEDLLNNPFNYEVDHIIPRSVSFDNSFNNKVLVKQ
                     EENSKKGNRTPFQYLSSSDSKISYETFKKHILNLAKGKGRISKTKKEYLLEERDINRFS
                     VQKDFINRNLVDTRYATRGLMNLLRSYFRVNNLDVKVKSINGGFTSFLRRKWKFKKERN
                     KGYKHHAEDALIIANADFIFKEWKKLDKAKKVMENQMFEEKQAESMPEIETEQEYKEIF
                     ITPHQIKHIKDFKDYKYSHRVDKKPNRELINDTLYSTRKDDKGNTLIVNNLNGLYDKDN
                     DKLKKLINKSPEKLLMYHHDPQTYQKLKLIMEQYGDEKNPLYKYYEETGNYLTKYSKKD
                     NGPVIKKIKYYGNKLNAHLDITDDYPNSRNKVVKLSLKPYRFDVYLDNGVYKFVTVKNL
                     DVIKKENYYEVNSKCYEEAKKLKKISNQAEFIASFYNNDLIKINGELYRVIGVNNDLLN
                     RIEVNMIDITYREYLENMNDKRPPRIIKTIASKTQSIKKYSTDILGNLYEVKSKKHPQI
                     IKKG"
     CDS             5243..5290
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5297..5377
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    5412..6000
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5465..5485)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5883..5894)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6091..6110)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6156..6211
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6210..6229)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6572..6588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6572..6588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6620..6626,6627..6644,6645..6650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    6775..6808
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7240..7257)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7411..7999)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7491..7510)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8170..8961,8962..9030))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8793..8812
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9031..9135)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctgggt cgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
      421 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt
      481 ttgtgcagcg atgggggcgg gggggggggg ggggcgcgcs ccgaggnncg ggggnggggg
      541 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      601 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      661 gggggggggg gggggggggs ssggssskks bkbbbsscss ssccsssssc cccsssggsc
      721 ccssscsssr rkttkgtkkt ktkttgtgtt ttwdtktttk tgggsggggg gggggggggg
      781 gggggggggg ggcgcgcgcs rggsggggcg gggcggggcg aggggcgggg cggggcgagg
      841 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
      901 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
      961 cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga
     1021 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat
     1081 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg
     1141 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1201 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
     1261 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
     1321 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
     1381 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga
     1441 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc
     1501 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg
     1561 gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc
     1621 gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct
     1681 ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg
     1741 cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     1801 tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc
     1861 tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct
     1921 agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg
     1981 ctggttattg tgctgtctca tcattttggc aaagaattcc tcgaccggtg ccaccatggc
     2041 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccaagc ggaactacat
     2101 cctgggcctg gacatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg
     2161 ggacgtgatc gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg
     2221 caggcggagc aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag
     2281 agtgaagaag ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat
     2341 caacccctac gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc
     2401 tgccgccctg ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga
     2461 ggacaccggc aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga
     2521 agagaaatac gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg
     2581 cagcatcaac agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt
     2641 gcagaaggcc taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga
     2701 aacccggcgg acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat
     2761 caaagaatgg tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag
     2821 cgtgaagtac gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt
     2881 gatcaccagg gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa
     2941 cgtgttcaag cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa
     3001 cgaagaggat attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct
     3061 gaaggtgtac cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga
     3121 gctgctggat cagattgcca agatcctgac catctaccag agcagcgagg acatccagga
     3181 agaactgacc aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct
     3241 gaagggctat accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga
     3301 gctgtggcac accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa
     3361 gaaggtggac ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct
     3421 gagccccgtc gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa
     3481 gaagtacggc ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga
     3541 cgcccagaaa atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga
     3601 ggaaatcatc cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct
     3661 gcacgacatg caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct
     3721 gctgaacaac cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga
     3781 caacagcttc aacaacaagg tgctcgtgaa gcaggaagaa aacagcaaga agggcaaccg
     3841 gaccccattc cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa
     3901 gcacatcctg aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct
     3961 gctggaagaa cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct
     4021 ggtggatacc agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt
     4081 gaacaacctg gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg
     4141 gaagtggaag tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct
     4201 gatcattgcc aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa
     4261 agtgatggaa aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac
     4321 cgagcaggag tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt
     4381 caaggactac aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga
     4441 caccctgtac tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa
     4501 cggcctgtac gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa
     4561 gctgctgatg taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca
     4621 gtacggcgac gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac
     4681 caagtactcc aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa
     4741 actgaacgcc catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa
     4801 gctgtccctg aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt
     4861 gaccgtgaag aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg
     4921 ctatgaggaa gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt
     4981 ctacaacaac gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa
     5041 cgacctgctg aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga
     5101 aaacatgaac gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag
     5161 cattaagaag tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca
     5221 ccctcagatc atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
     5281 aaagaaaaag ggatcctacc catacgatgt tccagattac gcttacccat acgatgttcc
     5341 agattacgct tacccatacg atgttccaga ttacgcttaa gaattcaagc ttcggggact
     5401 agtcatatga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5461 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5521 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5581 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5641 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5701 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5761 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     5821 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5881 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5941 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     6001 atcggtacaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     6061 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     6121 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     6181 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     6241 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     6301 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     6361 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     6421 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     6481 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     6541 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6721 gaaacctgtc gtgccagcgg atctcaattc cgatcatatt caataaccct taatataact
     6781 tcgtataatg tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag
     6841 cttaggatca attctcatgt ttgacagctt atcatcgata agctgatcct cacaggccgc
     6901 acccagcttt tcttccgttg ccccagtagc atctctgtct ggtgaccttg aagaggaaga
     6961 ggaggggtca aggatgtcaa ctacagtcag agaacccctt tgtgtttggt ccccccccgt
     7021 gtcacatgtg gaacagggcc cagttggcaa gttgtaccaa ccaactgaag ggattacatg
     7081 cactgccccg cgaagaaggg gcagagatgt cgtagtcagg tttagttcgt ccggggcggg
     7141 gatcgatcct ctagagtcga cgctagcgga tccgctgcat taatgaatcg gccaacgcgc
     7201 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7261 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7321 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7381 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7441 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7501 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7561 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7621 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7681 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7741 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7801 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7861 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7921 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7981 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8041 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8101 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8161 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8221 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8281 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8341 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8401 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8461 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     8521 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8581 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8641 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8701 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8761 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     8821 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8881 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8941 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9001 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9061 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9121 aataggggtt ccgcgcacat ttccccgaaa agtg
//
