LOCUS       sequence-269750-        3459 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus sequence of Hop (Humulus lupulus) tandem repeats for 
            cytogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Easterling KA, Pitra NJ, Morcol TB, Aquino JR, Lopes LG, Bussey KC, 
            Matthews PD, Bass HW
  TITLE     Identification of tandem repeat families from long-read sequences of
            Humulus lupulus.
  JOURNAL   PLoS One. 2020 Jun 5;15(6):e0233971. doi: 
            10.1371/journal.pone.0233971. eCollection 2020.
  PUBMED    32502183
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-06-5"; volume: "15(6):e0233971. doi"; pages: " 
            10.1371/journal.pone.0233971. eCollection 2020"
COMMENT     Sequence Label: pHTR400syn
FEATURES             Location/Qualifiers
     source          1..3459
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     10..29
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     223..245
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     237..254
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     238..254
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1097..1113)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1097..1113)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1110..1132)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1121..1137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1145..1151,1152..1169,1170..1175))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1190..1211
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1328..1345)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1499..2087)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1579..1598)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2258..3049,3050..3118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2881..2900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3119..3223)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3291..3309
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3347..3369)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
       61 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
      121 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      181 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      241 aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga ctctagagga tccccataaa
      301 aatacattat taaatatatt ctaatttgtt tatattattg aaaatttaaa atatattaat
      361 ttatttaaac aatacatata taaaatttat ttgggtatga aataagtata aaaaatatat
      421 aattattaat actacatata ttataatttt aaatatgaat aaattaataa attatttata
      481 attaaataaa tttatttcat ttttataaaa aagaaaatat tttaatttaa taatagtgat
      541 aatttaaaat aaaaaaattt ataacataat ttagtgtatc tctttaataa ttttttagag
      601 gacatggtag gtcggttgtg tgggaaatga cttctcatgt gacatggtag gtcggtttgt
      661 gttgaaccga cctaccatgt catttaacat ggtaggtcgg ttgtgtgagg aatgattctc
      721 atgtgatcat ggtagtcggt tgtgtgggaa cgacctacca tgtcattata aaatatatta
      781 ttaaatatac tctaattttt ttttggatta ttggaatttt aaaaatatat taatttattt
      841 aaaaatacat atatataatt tatttgggtg tggaataagt attaaatata tatagttatt
      901 aatattatat attataattt ttgaaatata aataaataaa ttattaataa ttaaaatata
      961 ataatttaat ttttataaat aagaaaatat ttgatttaat ggtatttgtg atttaaaata
     1021 aaagaactta ttaaaaaatt gtagagtatc tctttaataa tttttaaagg gtaccgagct
     1081 cgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     1141 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1201 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1321 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1381 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1441 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1501 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1561 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1621 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1681 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1741 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1801 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1861 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1921 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1981 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2041 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2101 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2161 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2221 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3181 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3241 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     3301 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     3361 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     3421 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggc
//
