LOCUS       sequence-269762-        2986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus sequence of Hop (Humulus lupulus) tandem repeats for 
            cytogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Easterling KA, Pitra NJ, Morcol TB, Aquino JR, Lopes LG, Bussey KC, 
            Matthews PD, Bass HW
  TITLE     Identification of tandem repeat families from long-read sequences of
            Humulus lupulus.
  JOURNAL   PLoS One. 2020 Jun 5;15(6):e0233971. doi: 
            10.1371/journal.pone.0233971. eCollection 2020.
  PUBMED    32502183
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-06-5"; volume: "15(6):e0233971. doi"; pages: " 
            10.1371/journal.pone.0233971. eCollection 2020"
COMMENT     Sequence Label: pHTR060syn
FEATURES             Location/Qualifiers
     source          1..2986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..39
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     31..48
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     32..48
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(418..434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(418..434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(431..453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    442..458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(466..472,473..490,491..496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    511..532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(649..666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(820..1408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(900..919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1579..2370,2371..2439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2202..2221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2440..2544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2612..2630
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2668..2690)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2790..2809
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagcttgca
       61 tgcctgcagg tcgactctag aggatccccg ggttataaca aagaggaccg agcccaggcc
      121 ggtcccaccc cggacgaacg acgtccgggg gtcgggtccc accccggacg aacgacgtcc
      181 gggggtatga aaaagaaacc gagctcaagg cccggacgaa cgatgtccgg gggtataatt
      241 tagaggaccg agccaaggcg gtcccacaag aggaccgagc caaggccggt cccacccctg
      301 gacgaacgac gtccgggggt ataacaggcc ggtcccaccc cggacgaacg atgtccgggg
      361 gtataaatat aagtggaccg agcccacccg ggtaccgagc tcgaattcgt aatcatggtc
      421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
      481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
      541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
      601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
      661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1261 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     2041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     2101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     2161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     2221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     2281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     2341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     2401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2581 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     2641 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     2701 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     2761 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2821 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     2881 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2941 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgg
//
