LOCUS       sequence-269790-        3280 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Consensus sequence of Hop (Humulus lupulus) tandem repeats for 
            cytogenetics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Easterling KA, Pitra NJ, Morcol TB, Aquino JR, Lopes LG, Bussey KC, 
            Matthews PD, Bass HW
  TITLE     Identification of tandem repeat families from long-read sequences of
            Humulus lupulus.
  JOURNAL   PLoS One. 2020 Jun 5;15(6):e0233971. doi: 
            10.1371/journal.pone.0233971. eCollection 2020.
  PUBMED    32502183
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-06-5"; volume: "15(6):e0233971. doi"; pages: " 
            10.1371/journal.pone.0233971. eCollection 2020"
COMMENT     Sequence Label: pHTR625syn
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..37
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     29..46
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     30..46
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(723..745)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    734..750
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(758..764,765..782,783..788))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    803..824
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(941..958)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1112..1700)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1192..1211)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1871..2662,2663..2731))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2494..2513
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2732..2836)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2904..2922
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2960..2982)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3082..3101
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttgcatg
       61 cctgcaggtc gactctagag gatccccacc cttccatatc taatttttgt tattgttaca
      121 actgaaaatt atggcaacta ctctgctttt cttttcctat ttatgagaat tactccattt
      181 tcttttcata tttgtgaaga tgacttatga agacaaattt cttctttctt cttttttttt
      241 tttattaaaa aaaaagagga aaacccccct tcaatttctt ttccttattt tatatattct
      301 ttgaaagcta agcttatgga aaagtaattt gatagccaat tgactattga gattaaacac
      361 actctttgct tactaaatga tgtattttct agtctacacc taacatgtct ctcattggga
      421 attgtctggt attcattcaa atcttctgct tggcatttaa tttaatcatt gagaaaataa
      481 tttttgatgc tttgaaagta gatctatttg ctggtggtgc ttaagtttaa tgaattatgc
      541 ttgagctagt gtttgttgct tgctggttgt tagttgagta ttgctataaa aggaagtagg
      601 cttgattgaa ccattggctt ttgttgcccg gatgttgcag aataaataga ttcttgcaac
      661 tagcttattt attttctata tgggtaccga gctcgaattc gtaatcatgg tcatagctgt
      721 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      781 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      841 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      901 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
      961 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1021 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1081 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     1141 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     1201 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     1261 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     1321 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     1381 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1441 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1501 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1561 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1621 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1681 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1741 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1801 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1861 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1921 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1981 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     2041 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     2101 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     2161 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     2221 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     2281 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     2341 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     2401 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     2461 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     2521 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     2581 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     2641 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2701 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2761 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2821 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     2881 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     2941 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     3001 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     3061 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     3121 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     3181 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     3241 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
//
