LOCUS       sequence-270082-        9551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one Sleeping Beauty vector for Expression for Tasmanian devil
            TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap 
            genes-of-interest (i.e. TNFRSF9)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9551)
  AUTHORS   Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De 
            Luca A, Lyons AB, Woods GM, Patchett AL
  TITLE     A novel system to map protein interactions reveals evolutionarily 
            conserved immune evasion pathways on transmissible cancers.
  JOURNAL   Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 
            10.1126/sciadv.aba5031. Print 2020 Jul.
  PUBMED    32937435
REFERENCE   2  (bases 1 to 9551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-07-1"; volume: "6(27). pii: 6/27/eaba5031. doi"; pages: 
            " 10.1126/sciadv.aba5031. Print 2020 Jul"
COMMENT     Sequence Label: pAF160
FEATURES             Location/Qualifiers
     source          1..9551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1130..1150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1211..1220
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1796..1816
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1850..1867
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             join(1874..1876,1877..2590)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNIYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FRTAAGITHGMDELYK"
     primer_bind     complement(1916..1937)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2177..2196)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(2642..2659)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2648..2872
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        3234..3537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3538..3741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3692..3712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3738..3762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          3879..3975
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     primer_bind     4032..4051
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4032..4050
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4061..5083
                     /codon_start=1
                     /product="hyperactive variant of the Sleeping Beauty 
                     transposase (Mates et al., 2009)"
                     /label=SB100X
                     /translation="MGKSKEISQDLRKRIVDLHKSGSSLGAISKRLAVPRSSVQTIVRK
                     YKHHGTTQPSYRSGRRRVLSPRDERTLVRKVQINPRTTAKDLVKMLEETGTKVSISTVK
                     RVLYRHNLKGHSARKKPLLQNRHKKARLRFATAHGDKDRTFWRNVLWSDETKIELFGHN
                     DHRYVWRKKGEACKPKNTIPTVKHGGGSIMLWGCFAAGGTGALHKIDGIMDAVQYVDIL
                     KQHLKTSVRKLKLGRKWVFQHDNDPKHTSKVVAKWLKDNKVKVLEWPSQSPDLNPIENL
                     WAELKKRVRARRPTNLTQLHQLCQEEWAKIHPNYCGKLVEGYPKRLTQVKQFKGNATKY
                     "
     polyA_signal    5100..5234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5124..5143)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5178..5197
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5387..5491
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5492..5560,5561..6352)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5710..5729)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6523..7111
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7012..7031
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7265..7282
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7656..7675
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    7674..7729
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     7775..7794
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(7894..8919)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7894..8919)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        join(9551,1..1182)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
ORIGIN
        1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
       61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
      121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
      181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
      241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
      301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
      361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
      421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
      481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
      541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
      601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
      661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
      721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
      781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
      841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
      901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
      961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     1021 atggagtttc cccatactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     1141 acagtggttc aaagtttttt tcttccattt aaggtgtcgt gaaaactacc ccaagctggc
     1201 ctctgcggcc gccaccatgg taaacagcta caatagactg gaggcgatat caactcttgt
     1261 tatactactc tgggtaactc tggtccaggg ttcctgtggc agctgtccag ctggtacttt
     1321 ctggaatggt acagattgtg gaaaaaacaa aaatcaagct tgcatcccat gtcccccaaa
     1381 tagtttctct agtacagctg gcgaacagag gacatgtaac atatgcagga gatgtgaagg
     1441 tatttttaga acaaagagag aatgtactcc aacaagtaat acagagtgtg attgtatgtc
     1501 tgggtatcat tgttccggag taggatgtaa taggtgtcaa ccagattgca agcaaggtca
     1561 agaacttcta ggaaaaggtt gtagagactg tagctttggt acctttaatg atcagacacg
     1621 ggggaactgc aaacgctgga caaactgctc tttgaatgga ctagttgtgc ttgtaaatgg
     1681 aaccaataaa agagatgtga tatgtggatc cacccaaaca gttgccactc tggacacaat
     1741 ctccatgaca attccagctt ctctcgtgca cattgataag aaccatcagc ccggggagaa
     1801 cctttacttc cagggatccg ctggctccgc tgctggttct ggcgaattcc atcatcacca
     1861 tcaccatgtc gacgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1921 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1981 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2041 ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag tgcttcagcc gctaccccga
     2101 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2161 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2221 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2281 cctggggcac aagctggagt acaacttcaa cagccacaac atctatatca tggccgacaa
     2341 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     2401 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2461 cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg agaagcgcga
     2521 tcacatggtc ctgctggagt tccgcaccgc cgccgggatc actcacggca tggacgagct
     2581 gtacaagtag gctagcgtca ggccaagctt ggggatcaat tctctagagc tcgctgatca
     2641 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     2701 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     2761 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     2821 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     2881 gcggaaagaa ccagctgggc ctactagata gcggacccct taccgaaaca tcgccgcatt
     2941 ctgcagagga gtcgaactta gtgtatgtaa acttctgacc cactggaatt gtgatacagt
     3001 gaattataag tgaaataatc tgtctgtaaa caattgttgg aaaaatgact tgtgtcatgc
     3061 acaaagtaga tgtcctaact gacttgccaa aactattgtt tgttaacaag aaatttgtgg
     3121 agtagttgaa aaacgagttt taatgactcc aacttaagtg tatgtaaact tccgacttca
     3181 actgtatagg gatccaattt taacaaaata ttcgagcttg catgcctgca ggtcgttaca
     3241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     3541 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     3601 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     3661 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     3721 gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca
     3781 cgctgttttg acctccatag aagacaccgg gaccgatcca gcctccggac tctagaggat
     3841 ccggtactcg aggaactgaa aaaccagaaa gttaactggt aagtttagtc tttttgtctt
     3901 ttatttcagg tcccggatcc ggtggtggtg caaatcaaag aactgctcct cagtggatgt
     3961 tgcctttact tctaggcctg tacggaagtg ttacttctgc tctaaaagct gcggaattgt
     4021 acccgcggaa ttaatacgac tcactatagg gactagtacc atgggaaaat caaaagaaat
     4081 cagccaagac ctcagaaaaa gaattgtaga cctccacaag tctggttcat ccttgggagc
     4141 aatttccaaa cgcctggcgg taccacgttc atctgtacaa acaatagtac gcaagtataa
     4201 acaccatggg accacgcagc cgtcataccg ctcaggaagg agacgcgttc tgtctcctag
     4261 agatgaacgt actttggtgc gaaaagtgca aatcaatccc agaacaacag caaaggacct
     4321 tgtgaagatg ctggaggaaa caggtacaaa agtatctata tccacagtaa aacgagtcct
     4381 atatcgacat aacctgaaag gccactcagc aaggaagaag ccactgctcc aaaaccgaca
     4441 taagaaagcc agactacggt ttgcaactgc acatggggac aaagatcgta ctttttggag
     4501 aaatgtcctc tggtctgatg aaacaaaaat agaactgttt ggccataatg accatcgtta
     4561 tgtttggagg aagaaggggg aggcttgcaa gccgaagaac accatcccaa ccgtgaagca
     4621 cgggggtggc agcatcatgt tgtgggggtg ctttgctgca ggagggactg gtgcacttca
     4681 caaaatagat ggcatcatgg acgcggtgca gtatgtggat atattgaagc aacatctcaa
     4741 gacatcagtc aggaagttaa agcttggtcg caaatgggtc ttccaacacg acaatgaccc
     4801 caagcatact tccaaagttg tggcaaaatg gcttaaggac aacaaagtca aggtattgga
     4861 gtggccatca caaagccctg acctcaatcc tatagaaaat ttgtgggcag aactgaaaaa
     4921 gcgtgtgcga gcaaggaggc ctacaaacct gactcagtta caccagctct gtcaggagga
     4981 atgggccaaa attcacccaa attattgtgg gaagcttgtg gaaggctacc cgaaacgttt
     5041 gacccaagtt aaacaattta aaggcaatgc taccaaatac taggggccct aaccgcgggg
     5101 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5161 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5221 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     5281 tgtgggaggt tttttaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg
     5341 tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     5401 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     5461 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     5521 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     5581 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     5641 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     5701 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     5761 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     5821 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     5881 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     5941 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6001 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6061 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6121 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6181 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6241 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6301 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6361 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6421 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6481 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6541 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6601 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6661 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6721 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6781 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6841 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6901 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6961 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7021 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7081 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7141 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7201 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7261 gcgcagcgag tcagtgagcg aggaagcgga tccctataca gttgaagtcg gaagtttaca
     7321 tacaccttag ccaaatacat ttaaactcac tttttcacaa ttcctgacat ttaatcctag
     7381 taaaaattcc ctgtcttagg tcagttagga tcaccacttt attttaagaa tgtgaaatat
     7441 cagaataata gtagagagaa tgattcattt cagcttttat ttctttcatc acattcccag
     7501 tgggtcagaa gtttacatac actcgactcc tctgcagaat gcggcgatgt ttcggtaagg
     7561 ggtcccgagt agctagttca tggcagccag catatggcat atgttgccaa actctaaacc
     7621 aaatactcat tctgatgttt taaatgattt gccctcccat atgtccttcc gagtgagaga
     7681 cacaaaaaat tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagtc
     7741 agatgctcaa ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag
     7801 tggtatttgt gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc
     7861 acctgaggag tgaattcacg acaggccttc gaactattcc tttgccctcg gacgagtgct
     7921 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     7981 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     8041 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     8101 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
     8161 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     8221 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     8281 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     8341 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     8401 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     8461 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     8521 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
     8581 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     8641 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
     8701 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     8761 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     8821 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     8881 cttctcgaca gacgtcgcgg tgagttcagg ctttttcatg gtggcctcag gtgcagaggt
     8941 ttctacaggg aaagagagaa aggctacagt tagtttgctg tgatgatgct aagtgccgag
     9001 gtcagatgtc tatggattgt acacggaatc ccaacgcagg aaccaaaaaa cactcaccca
     9061 aaaaaatggc gctaaggccg agagaaaggc gaagttccgt ctacggctat ataccttgaa
     9121 acaggggcgg cagaagtgac gcaacgcggt tgcgcggacg gcttcctgtg ttttcattgg
     9181 tcgaagcgtt ctgcgcgtgc gcagagattc cccagggaag gcgggccctc ttccgaatgt
     9241 ccaatccgga cattcttccg gttggacctc cgcggtcagc gctgtgcccg gcgagagatc
     9301 acgtggggcg cggaggcggt gctgctgggg cacggccgtc cagcctcggc ggccatattt
     9361 ttgaggggct gttcatctcg ttcacacgct ctgtccgcca tgtttgtgag tggaagcgcc
     9421 attacccctt caagcgactg aaggctgcag ggcctctggt ggcccgcatg gggagaccag
     9481 acccgccagg cccgcctttc cgcactcagt ccgggcttac tttattttgt gagacagggt
     9541 ctcgcctaga g
//
