LOCUS       sequence-270207-        5858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prey vector SERK2 P5_pECIA14 should be used with bait vector SERK2 
            P5_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 5858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: SERK2 P5_pECIA14
FEATURES             Location/Qualifiers
     source          1..5858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             1154..1177
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2852..2875
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2891..2908
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2931..2948)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2981..3115
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3005..3024)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3059..3078
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3259..3275)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3259..3275)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3272..3294)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3283..3299
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3307..3313,3314..3331,3332..3337))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3352..3373
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3490..3507)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3661..4249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3741..3760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4420..5211,5212..5280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5043..5062
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5281..5385)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5453..5471
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5509..5531)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5631..5650
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(5844..5858,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg tcttctaaca tggaaggtga
      541 tgcactgcac agtttgagag ctaatctagt tgatccaaat aatgtcttgc aaagctggga
      601 tcctacgctt gttaatccgt gtacttggtt tcacgtaacg tgtaacaacg agaacagtgt
      661 tataagagtc gatcttggga atgcagactt gtctggtcag ttggttcctc agctaggtca
      721 gctcaagaac ttgcagtact tggagcttta tagtaataac ataaccgggc cggttccaag
      781 cgatcttggg aatctgacaa acttagtgag cttggatctt tacttgaaca gcttcactgg
      841 tccaattcca gattctctag gaaagctatt caagcttcgc tttcttcggc tcaacaataa
      901 cagtctcacc ggaccaattc ccatgtcatt gactaatatc atgacccttc aagttttgga
      961 tctgtcgaac aaccgattat ccggatctgt tcctgataat ggttccttct cgctcttcac
     1021 tcccatcagt tttgctaaca acttggatct atgcggccca gttactagcc gtccttgtcc
     1081 tggatctccc ccgttttctc ctccaccacc ttttatacca cctcccatag ttcctacacc
     1141 aggtgggtat agtctggagg tgctgttcca gggaccaagc ggcaatcccg gcggcgatct
     1201 ggccccccag atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga
     1261 gctgctgcgc caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga
     1321 cgcctgcggc atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa
     1381 cccggacttc tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc
     1441 tgcacagaca gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac
     1501 ggtgacagct gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc
     1561 cctggccatg gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca
     1621 tgtgccagac agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca
     1681 gaccattggc ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga
     1741 ggtcatctcc gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac
     1801 cacacgagtg cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg
     1861 gtactcggac gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac
     1921 gcagctcatc tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt
     1981 tcgcatggga accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct
     2041 ggacgggaag aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg
     2101 gaaccgcact gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct
     2161 ctttgagcct ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct
     2221 gatggagatg acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct
     2281 cttcgtggag ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact
     2341 gactgagacg atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga
     2401 ggacacgctg agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc
     2461 cctgcgaggg agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta
     2521 cacggtcctc ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga
     2581 tgttaccgag agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga
     2641 cgaagagacc cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct
     2701 ggttcacggc gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct
     2761 ggagccctac accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc
     2821 gggttctaga ggaagcacct ccggcgataa cgattacaag gatgatgatg ataaggccag
     2881 cggcaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt
     2941 gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata
     3001 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     3061 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3121 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     3181 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgacct gcaggcatgc
     3241 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     3301 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3361 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3421 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3481 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3541 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3601 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3661 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3721 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3781 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3841 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3901 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3961 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4021 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4081 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4141 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4201 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4261 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4321 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4381 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4441 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4501 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4561 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4621 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4681 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4741 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4801 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4861 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4921 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4981 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5041 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5101 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5161 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5221 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5281 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5341 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5401 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5461 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5521 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5581 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5641 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5701 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5761 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     5821 aagttgggta acgccagggt tttcccagtc acgacgtt
//
