LOCUS       sequence-270379-        4021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, CPH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: P364
FEATURES             Location/Qualifiers
     source          1..4021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2074..2178
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3246..3834
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3735..3754
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3988..4005
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacctgctca tgatcggcgc gccctatgga gaatatcatt tttaagtaaa attttgaatt
       61 caaatgttac gtgtattatt taattcatca atttgccttg tcatagcgag tacattacaa
      121 acatcacata tatttgattg attgtcaaaa aatatcaaaa tatatatcaa ttttaagagg
      181 tataggtgtc taatatgtac tagccctaat ttaaatatct aaattaatta ttcggatgaa
      241 tctatatacc atctttttaa tggacaccca aaatcacaca tcaaacatca tatacatgtt
      301 gaaaacatat tattgatata gctacatata tgttttaata taaataaaag acgagtcata
      361 tattcaaaaa ttaagaatca aataatttta atttatttaa tattcaaaac ttaatactat
      421 ttaaatttag atattctaat tttaatacac gtctgataaa atagatgagg actaaataaa
      481 taatttgaga ctatcttttc tttatttggc ggcccacaaa taatttagat tctcgtaacc
      541 ccctcttttt ctctcactga aaaagcacaa tccgtgtcca aacacaaaga agcactcgac
      601 accgtagatc tccattcaga tcaacggctt atattcagtt ttctccattc acgtggatcg
      661 acattcttat ccgtccgatt atcaataaat ttcccaaaat ttagcggcca tgattttaac
      721 cccgcctcat ttcaaaccgc ccacgaaatc ctcgacgccc aaattcacca actataaata
      781 gccaccacca tccccttcat caatcatcaa atttcataac cctagaatca tcaccttttt
      841 caaatttcaa tggctcctat ctcctccacc tggtccagat taatcaggtt cgtagctata
      901 gaaacgtcac tcgtgcatat aggcgaaccg attgatgcaa ctatggacgt agggcttgct
      961 cgtcgagaag ggaaaactat tcaggcctat gagattatcg gatctggcag cgccctggac
     1021 ctttctgcac aggtgagtaa gaacgtgctc acagtacgag aattgttgat gccactttct
     1081 agagaagaga taaagacggt cagatgctta ggccttaact atcctgtcca cgcaacagaa
     1141 gccaatgttg ctgtaccgaa gtttccgaac ctgttctaca aaccagtcac aagtctcatc
     1201 gggcctgatg gtcttatcac gatccctagt gtagtgcaac cgcctaagga acatcagtca
     1261 gattatgaag ctgaacttgt gattgtaatc ggaaaggccg ccaagaacgt gtctgaggac
     1321 gaagccttgg attatgtctt aggctataca gctgctaacg atattagttt ccgaaaacac
     1381 cagctcgcag tctcacagtg gtcattctca aaaggatttg gatctctcct tctcactata
     1441 cgtatggccc agacacatag tggaaacata aataggttct cacgtgatca aatctttaat
     1501 gttaagaaaa caatcagctt tttgtctcaa ggaactaccc tcgaacctgg gtctatcatt
     1561 ttgactggca cccctgatgg ggtcggcttc gtcaggaacc cgcccttata cttaaaagat
     1621 ggggatgaag tgatgacttg gataggttcc ggcattggta cgcttgctaa caccgttcaa
     1681 gaagagaaaa catgctttgc aagcggtggg catgagtagg cttttatgtt ggtgatatgg
     1741 tggtaaatgt agggatttag tttacaattg cgtatgtctg tgttggatat ctgtagtgct
     1801 gttcttatgg cttagatctt gtaatttctc attacagtat caatgaatag atatcagttt
     1861 ctagtgatga cattggttcg tcttttagct gttgattaat ttttcttaat tgattcatcc
     1921 tattgcaatt cttctgaatt taaattgtat actgtgaaat taagaaaatt cttgaaatta
     1981 atgagaattt gagtaatagc gctgttagag ctctgacgtg ggcaggtgtg atgaaaagcc
     2041 ttagggtcag gtggcacttt tcggggaaat gtgcgatgaa cccctatttg tttatttttc
     2101 taaatacatt caaatatgta tccgctaatg agacaataac cctgataaat gcttcaatta
     2161 tattgaaaaa ggaagagtat gagtatccaa catttccgtg tcgcccttat tccctttttt
     2221 gcggccttct gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2281 gaagatcagt tgggtgcccg agtgggttac attgaactgg atctcaacag cggcaaaatc
     2341 ttagagagtt ttcgccccga agaacgcttc ccaatgatga gcactttcaa agttctgcta
     2401 tgtggcgcgg tgttatcccg tattgatgct gggcaagagc aactcggtcg ccgcatacac
     2461 tattctcaga atgacttggt tgagtattca ccagtcacag aaaagcatct tacggatggc
     2521 atgacagtaa gagaattatg tagtgctgcc ataaccatga gtgataacac tgcggccaac
     2581 ttacttctga caacgattgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     2641 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     2701 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaactc tccgcaaact attaactggc
     2761 gaactactta ctcttgcttc acgccagcaa ctcattgact ggatggaggc ggacaaagtt
     2821 gcaggaccac ttctgcgctc ggcacttccg gctggctggt ttattgctga taaatctgga
     2881 gccggtgagc gtggctctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     2941 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3001 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacga agggaaataa
     3061 atagtagccc gcctgatgtg cgggcttttt tttggtggtg gttaccagtg tcgcttagct
     3121 accgtaggtc ctcttaggcg gtccgattag cgacatagtc atcaatgcct tgacggcaaa
     3181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3301 ctaccagcgg tggtttgttt gccggatcaa gagctactaa ctctttttcc gaaggtaact
     3361 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgct cacagcccag cttggagcga
     3601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3901 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3961 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4021 c
//
