LOCUS       sequence-270389-        7868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Hisps.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO488
FEATURES             Location/Qualifiers
     source          1..7868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      5600..5848
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        5921..6025
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7093..7681
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7582..7601
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7835..7852
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cacctgcaac gccggggcgc gccctatgga gtggcagaca tactgtccca caaatgaaga
       61 tggaatctgt aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc
      121 tttaatcaat accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt
      181 tttctgacga acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc
      241 ttcagactcc aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg
      301 ggaaatgtcc actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga
      361 gcaaaatctc agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc
      421 aaggcggcga agtattcagg cacgtggcca ggaagaagaa aagccaagac gacgaaaaca
      481 ggtaagagct aagcttaatg aatagtagta aaaaccctcc atccactctg ttagatgttt
      541 ttctggatac cgctaggaac cttgatacag catccagaaa tgtcctggag tgcggagaac
      601 acaggtggag ttacagagag ttggacacag tgtcatccgc attggcccaa caccttcgat
      661 acactgtggg tcttagcccc actgtagccg tgattagcga gaatcacccc tacatacttg
      721 cattaatgct cgccgtatgg aaactcggcg gaacgttcgc accgatagat gttcattccc
      781 cagccgaact tgttgctgga atgttgaata ttgtaagccc gtcatgtctt gtcattccta
      841 gctctgatgt gacaaatcag acattggcat gcgatcttaa tataccggtt gttgcattcc
      901 acccacatca aagtacaatt cccgaactca acaagaagta tctcacagat tctcagatca
      961 gtcctgacct gcccttcccc gaccctaatc gacctgcttt gtacttattc acgtcatctg
     1021 caacgtctag gtccaatttg aagtgcgtgc cacttacaca cacattcata ttgcgaaact
     1081 ccttaagtaa acgagcatgg tgcaaaagaa tgaggccaga gacagatttt gatggtatta
     1141 gggtcctggg gtgggccccc tggagccatg tcctcgctca tatgcaggac attggccctc
     1201 ttacattatt gaatgccggg tgctacgtat ttgccacgac cccatctacc tacccgacgg
     1261 agctgaagga tgaccgtgat gttattagtt gcgccgcaaa tgccgtaatg tacaaaggtg
     1321 ttaaatcctt cgcatgcctc ccattcgtgc ttgggggact caaggcttta tgtgagtcag
     1381 agcccagtgt taaggcacaa ctgcaagtgg aggaacgagc ccagcttttg aagtcactcc
     1441 aacacatgaa aatcctggaa tgcggggggg caatgctcga agtgagtgtg gcaagctggg
     1501 ctatagagaa taggattcct atatctattg ggattggtat gacagagaca ggcggcgctc
     1561 ttttcgctgg gccggtccaa gcaattcaaa ccggctttag cagtgaagat aaatttatcg
     1621 aggacgcaac ctacttgctg gtcaaggacg attacgagag ccacgcagag gaggacataa
     1681 acgagggcga gttagtggta aagtccagaa tgttacctag gggttatttg ggctataacg
     1741 acccctcttt ctctgttgac gatgctggtt gggttacttt caaaactgga gatcgatatt
     1801 cagtcactcc tgacggcaaa ttctcctggc ttggtcgaaa tactgatttc attcagatga
     1861 cgagtggtga gactctcgat ccccgtccga ttgagtccct cctttgtgag tccagcctta
     1921 tatcacgagc ctgtgtcatc ggggataagt tcctgaacgg accagccaca gctgtatgcg
     1981 ctattataga actcgaacca acgactgttg agaaaggcca ggctcacagc agggatattg
     2041 cccgaatctt tgcccccatc aatcgagatc ttcctccccc gttacgtata gcatggagtc
     2101 acgtacttgt acttcagcca agcgagaaaa taccgatgac gaaaaagggt acaatcttcc
     2161 gtaagaaaat cgaacaggtt ttcggttccg ctttaggagg cagtagcggc gacaactcac
     2221 aagcaaccac tgacgcttca gtagttagga gggatgagtt gagcaacacg gtcaaacata
     2281 tcataagcag agttttggga gtctccgacg atgaactcct ttggacactg agctttgctg
     2341 agcttggcat gacgtccgct cttgctacta gaatagcaaa tgaattgaac gaagttctgg
     2401 taggtgtgaa cctccccatc aatgcatgct acattcatgt ggacctgcct tctttgtcaa
     2461 atgccgtgta tgcaaagtta gcacacctta aactcccgga taggacgccc gagccaagga
     2521 aggccccagt tgaaaatccc ggtggaaagg aaatcgtaat agtaggtcag gcttttaggc
     2581 ttccggggag tataaatgat gtcgcttctt tgagggacgc ttttttggct agacaagcat
     2641 ccagtattat tactgaaata ccgccagata gatgggacca cgcatcattt tatccaaaag
     2701 acatacgatt taataaagca gggctcgtcg acatcgctaa ctacgaccac agttttttcg
     2761 gattgacagc cacggaggca ctgtacctgt caccaacgat gcgtttggca cttgaagtgt
     2821 cctttgaagc cttggagaat gctaatatac ccgtgtccca gttaaagggc tctcaaacag
     2881 ctgtatatgt cgcaaccacc gacgacgggt ttgagacact gctgaatgct gaggcaggat
     2941 atgatgcata cacacgattc tatggcactg gtagggccgc ctctacggcc agtggacgaa
     3001 ttagttattt actcgacgta cacggccctt caattaccgt agacactgct tgttcaggcg
     3061 gtgctgtttg catagaccag gccatagact acctccaatc ctctagcgca gctgataccg
     3121 ccataatctg cgccagcaac actcactgct ggccaggttc cttcatgttt ctgagtgctc
     3181 aaggtatggt aagttccggg gggcgatgtg caacttttac gacggatgcc gacggctatg
     3241 taccctctga aggtgctgtg gcatttatcc tcaaaacccg agaagcagct atgagagata
     3301 aagatactat cttggcaact atcaaagcaa cgcagattag tcataatggt cgttcccaag
     3361 gtttagtcgc accaaacgtc aattcacaag ctgatttaca cagaagctta ttgcagaaag
     3421 ctggtttgag tccagccgac attcacttca tcgaagctca cgggacagga acgtcactcg
     3481 gtgaccttag tgaaatacaa gcaattaatg atgcctacac gtcatctcag cctcgaacag
     3541 ccggaccctt aatagtaagc gcaagtaaaa ccgtcattgg tcacacagag cctgctggac
     3601 cgttggtagg catgctctca gttctcaact cctttaaaga gggcgcagtt cccggcttag
     3661 ctcacctgac agcagataac ctcaatccgg ctttagactg ctcctccgtc cctttactta
     3721 tcccctacca gcctgtccac ttggcagctc ctaagccaca cagggctgct gtgatgagtt
     3781 atggcttctc cggcactctt ggaggtatag ttcttgaagc accggatgag gaaaggcttg
     3841 aggaagaacc acccaacgat aagcctatgt tatttgttgt tagcgccaaa actcatactg
     3901 cattgattga gtaccttgga cgataccttg aatttctgct ccaggccaat ccccaagact
     3961 tctgcgatat ctgttacact agctgcgttg gacgtgagca ttacaggtac cgttttgcat
     4021 gtgtggcaaa cgatatggaa gatttaattg gccaacttca gaagaggctt gggtctaagg
     4081 ttccacctaa accgtcctat aaaagaggag ccctcgcttt tgctttcagt ggacaaggga
     4141 cccagtttcg tggaatggca acagaattgg ctaaggcata tagtggtttt cgtaagatag
     4201 tcagtgatct tgctaaaaga gcatcagaac tgtccggcca tgcaatcgac aggtttctgc
     4261 ttgcatatga cattggggct gagaatgttg caccggatag tgaagctgat caaatctgca
     4321 tattcgttta ccaatgtagt gttctcagat ggctccaaac aatggggatt cgtccgtcag
     4381 ccgtgatcgg tcacagcctc ggtgagatta gcgcatccgt agctgctgga gcattgtcct
     4441 tggactctgc ccttgacctc gtaataagta gagccaggtt actgaggtca tctacgaatg
     4501 ccccagctgg catggcagct atgtctgcat cccaggatga agtagtcgaa ctcataggaa
     4561 agctcgattt agataaagct aatagcctca gcgtgtccgt aataaacggc ccccagaaca
     4621 ccgttgtttc aggatcttct gcagcaatag aaagtatcgt tgctctcgca aagggacgaa
     4681 aaatcaaggc cagcgcactc aacattaacc aggcattcca tagtccttac gtcgattctg
     4741 cagtacccgg cttacgagct tggagtgaga aacatatatc atcagctcga ccattgcaaa
     4801 tccctttgta ctctaccctc ttgggcgctc aagtttctga gggtcaaatg ttaaatcccg
     4861 atcactgggt agaccatgct aggaaacccg ttcagttcgc acaagcagcc actataatga
     4921 aggaatcttt caccggagta ataatcgata ttggacctca agttgtagca tggtccctgc
     4981 tcttaagcaa tggtcttact tccgtgaccg cactcgccgc aaagcgtggc cgttcacagc
     5041 aagtggcctt cctctccgcc ttagctgatc tttatcagga ctacggcgtc gtaccagact
     5101 ttgttggact gtacgcacaa caggaggacg cttccagact gaaaaagacc gatatcttaa
     5161 cttacccctt tcagagggtt cgacgttacc cttcctttat accctctaga agggcaccca
     5221 cccacgctca tgtccaagac gaggaaactc tgtcatcagg atcatcaact ccaaccttag
     5281 aaaatactga cctggacagt gggaaggaat ctctgatggg accaacaaga gggcttttgc
     5341 gagtcgatga tcttagggac tcaatagtca gttcagtcaa agatgtcctg gaattgaaga
     5401 gtaacgaaga tctggacctc agcgagtccc ttaatgctct tggcatggac tctataatgt
     5461 tcgctcaatt acgaaaaagg ataggggagg gcctcggcct gtccgttcca atggtttttc
     5521 tcagcgatgc cttcagtatt ggagaaatgg tcagtaacct cgtggaacag gccgaagctt
     5581 ccgaggacaa ttgagcttat atgaagatga agatgaaata tttggtgtgt caaataaaaa
     5641 gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt
     5701 ttctaatatt aaatgaatgt aagatcacat tataatgaat aaacaaatgt ttctataatc
     5761 cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta
     5821 tggactatgg aatatgatta aagataagcg ctgttagagc tctcccacta gcaggtgatg
     5881 aaaagcctta gggtcaggtg gcacttttcg gggaaatgtg cgatgaaccc ctatttgttt
     5941 atttttctaa atacattcaa atatgtatcc gctaatgaga caataaccct gataaatgct
     6001 tcaattatat tgaaaaagga agagtatgag tatccaacat ttccgtgtcg cccttattcc
     6061 cttttttgcg gccttctgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6121 agatgctgaa gatcagttgg gtgcccgagt gggttacatt gaactggatc tcaacagcgg
     6181 caaaatctta gagagttttc gccccgaaga acgcttccca atgatgagca ctttcaaagt
     6241 tctgctatgt ggcgcggtgt tatcccgtat tgatgctggg caagagcaac tcggtcgccg
     6301 catacactat tctcagaatg acttggttga gtattcacca gtcacagaaa agcatcttac
     6361 ggatggcatg acagtaagag aattatgtag tgctgccata accatgagtg ataacactgc
     6421 ggccaactta cttctgacaa cgattggagg accgaaggag ctaaccgctt ttttgcacaa
     6481 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6541 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaactctcc gcaaactatt
     6601 aactggcgaa ctacttactc ttgcttcacg ccagcaactc attgactgga tggaggcgga
     6661 caaagttgca ggaccacttc tgcgctcggc acttccggct ggctggttta ttgctgataa
     6721 atctggagcc ggtgagcgtg gctctcgcgg tatcattgca gcactggggc cagatggtaa
     6781 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6841 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagacgaagg
     6901 gaaataaata gtagcccgcc tgatgtgcgg gctttttttt ggtggtggtt accagtgtcg
     6961 cttagctacc gtaggtcctc ttaggcggtc cgattagcga catagtcatc aatgccttga
     7021 cggcaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7081 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7141 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctactaactc tttttccgaa
     7201 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     7261 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     7321 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     7381 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgctcac agcccagctt
     7441 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     7501 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     7561 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     7621 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7681 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7741 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     7801 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     7861 agagcgcc
//
