LOCUS       sequence-270390-        3549 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module plasmid, individual component of Fungal Bioluminescent 
            Pathway, Luz.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Khakhar A, Starker CG, Chamness JC, Lee N, Stokke S, Wang C, Swanson
            R, Rizvi F, Imaizumi T, Voytas DF
  TITLE     Building customizable auto-luminescent luciferase-based reporters in
            plants.
  JOURNAL   Elife. 2020 Mar 25;9. pii: 52786. doi: 10.7554/eLife.52786.
  PUBMED    32209230
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-03-25"; volume: "9. pii: 52786. doi"; pages: " 
            10.7554/eLife.52786"
COMMENT     Sequence Label: pCO489
FEATURES             Location/Qualifiers
     source          1..3549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     49..66
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        116..496
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        1684..1788
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2856..3444
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3345..3364
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
       61 agcgaggaag cggaagagcg cccacctgca acgtcccggc gcgccctatg gagatttttc
      121 aaatcagtgc gcaagacgtg acgtaagtat ccgagtcagt ttttattttt ctactaattt
      181 ggtcgtttat ttcggcgtgt aggacatggc aaccgggcct gaatttcgcg ggtattctgt
      241 ttctattcca actttttctt gatccgcagc cattaacgac ttttgaatag atacgctgac
      301 acgccaagcc tcgctagtca aaagtgtacc aaacaacgct ttacagcaag aacggaatgc
      361 gcgtgacgct cgcggtgacg ccatttcgcc ttttcagaaa tggataaata gccttgcttc
      421 ctattatatc ttcccaaatt accaatacat tacactagca tctgaatttc ataaccaatc
      481 tcgatacacc aaatcgaatg cgtataaata tctctctgtc tagcttattc gagagattaa
      541 gcaaactcag ctctcgtagc atagctatta catgtggtgt cgtcctcgcc tctgctattg
      601 cttttcccat catacgacga gattatcaga cgttcttaga ggttggtcct agctatgccc
      661 ctcaaaattt tcgaggctac attatcgttt gcgtactcag cttgttccga caagagcaaa
      721 aagggttggc aatctacgac agactcccag agaagagaag atggctggcc gatctcccat
      781 ttcgtgaagg tactagacct agtataacat cacacataat acagagacag cgaactcaac
      841 ttgtagacca agagtttgcc acacgagaac tgatagacaa agttattccc agggtgcaag
      901 cacgacatac agataaaaca tttttgagca cgagcaagtt tgaatttcat gctaaagcca
      961 ttttcctgtt gccaagtatt cctataaacg accccttaaa catacctagt catgatacgg
     1021 ttcgtagaac gaaacgagag attgcacaca tgcatgacta ccacgactgt actcttcact
     1081 tagctcttgc agctcaagat ggaaaagaag tcctgaaaaa aggctggggc caacgtcatc
     1141 cgcttgccgg gccgggtgta cctgggccgc cgacagaatg gactttctta tacgcacccc
     1201 gtaatgagga agaagctagg gtagtagaaa tgattgtcga ggcaagtatc ggatacatga
     1261 ctaatgatcc ggcaggtaaa atcgtcgaga atgccaaatg agcttgttcg agtattatgg
     1321 cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact gtgtttttta
     1381 ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta atgaatgata
     1441 tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt tgttgtgtgt
     1501 tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat gtgtcaaatc
     1561 gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg tcgctgttag
     1621 agctcagtga ctagcaggtg atgaaaagcc ttagggtcag gtggcacttt tcggggaaat
     1681 gtgcgatgaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctaatg
     1741 agacaataac cctgataaat gcttcaatta tattgaaaaa ggaagagtat gagtatccaa
     1801 catttccgtg tcgcccttat tccctttttt gcggccttct gccttcctgt ttttgctcac
     1861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcccg agtgggttac
     1921 attgaactgg atctcaacag cggcaaaatc ttagagagtt ttcgccccga agaacgcttc
     1981 ccaatgatga gcactttcaa agttctgcta tgtggcgcgg tgttatcccg tattgatgct
     2041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtattca
     2101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg tagtgctgcc
     2161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgattgg aggaccgaag
     2221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2341 gcaacaactc tccgcaaact attaactggc gaactactta ctcttgcttc acgccagcaa
     2401 ctcattgact ggatggaggc ggacaaagtt gcaggaccac ttctgcgctc ggcacttccg
     2461 gctggctggt ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt
     2521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2641 cattggtaac tgtcagacga agggaaataa atagtagccc gcctgatgtg cgggcttttt
     2701 tttggtggtg gttaccagtg tcgcttagct accgtaggtc ctcttaggcg gtccgattag
     2761 cgacatagtc atcaatgcct tgacggcaaa atcccttaac gtgagttttc gttccactga
     2821 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2881 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2941 gagctactaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     3001 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     3061 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     3121 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3181 ggttcgtgct cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3241 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3301 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3361 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3421 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3481 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3541 cgtattacc
//
