LOCUS       sequence-270454-        8815 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  V5-His-tagged gene expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8815)
  AUTHORS   Yadav L, Pietila E, Ohman T, Liu X, Mahato AK, Sidorova Y, Lehti K, 
            Saarma M, Varjosalo M
  TITLE     PTPRA Phosphatase Regulates GDNF-Dependent RET Signaling and 
            Inhibits the RET Mutant MEN2A Oncogenic Potential.
  JOURNAL   iScience. 2020 Feb 21;23(2):100871. doi: 10.1016/j.isci.2020.100871.
            Epub 2020 Jan 31.
  PUBMED    32062451
REFERENCE   2  (bases 1 to 8815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2020-02-21"; volume: "23(2):100871. doi"; pages: " 
            10.1016/j.isci.2020.100871. Epub 2020 Jan 31"
COMMENT     Sequence Label: MEN2A-V5
FEATURES             Location/Qualifiers
     source          1..8815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    911..935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4152..4176)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4193..4234
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4241..4282
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4313..4354
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4364..4381
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4404..4421)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4410..4634
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4680..5108
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4767..4786)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4977..4998
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5117..5137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5122..5451
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5302..5437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5364..5383
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5518..6312
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5572..5591)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6182..6201
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6488..6609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6525..6544)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6579..6598
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6658..6674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6658..6674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6671..6693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6682..6698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6706..6712,6713..6730,6731..6736))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6751..6772
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6889..6906)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7060..7648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7140..7159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7819..8610,8611..8679))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8442..8461
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8680..8784)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagctatca acaagtttgt acaaaaaagt tggcaccatg gcgaaggcga cgtccggtgc
      961 cgcggggctg cgtctgctgt tgctgctgct gctgccgctg ctaggcaaag tggcattggg
     1021 cctctacttc tcgagggatg cttactggga gaagctgtat gtggaccagg cggccggcac
     1081 gcccttgctg tacgtccatg ccctgcggga cgcccctgag gaggtgccca gcttccgcct
     1141 gggccagcat ctctacggca cgtaccgcac acggctgcat gagaacaact ggatctgcat
     1201 ccaggaggac accggcctcc tctaccttaa ccggagcctg gaccatagct cctgggagaa
     1261 gctcagtgtc cgcaaccgcg gctttcccct gctcaccgtc tacctcaagg tcttcctgtc
     1321 acccacatcc cttcgtgagg gcgagtgcca gtggccaggc tgtgcccgcg tatacttctc
     1381 cttcttcaac acctcctttc cagcctgcag ctccctcaag ccccgggagc tctgcttccc
     1441 agagacaagg ccctccttcc gcattcggga gaaccgaccc ccaggcacct tccaccagtt
     1501 ccgcctgctg cctgtgcagt tcttgtgccc caacatcagc gtggcctaca ggctcctgga
     1561 gggtgagggt ctgcccttcc gctgcgcccc ggacagcctg gaggtgagca cgcgctgggc
     1621 cctggaccgc gagcagcggg agaagtacga gctggtggcc gtgtgcaccg tgcacgccgg
     1681 cgcgcgcgag gaggtggtga tggtgccctt cccggtgacc gtgtacgacg aggacgactc
     1741 ggcgcccacc ttccccgcgg gcgtcgacac cgccagcgcc gtggtggagt tcaagcggaa
     1801 ggaggacacc gtggtggcca cgctgcgtgt cttcgatgca gacgtggtac ctgcatcagg
     1861 ggagctggtg aggcggtaca caagcacgct gctccccggg gacacctggg cccagcagac
     1921 cttccgggtg gaacactggc ccaacgagac ctcggtccag gccaacggca gcttcgtgcg
     1981 ggcgaccgta catgactata ggctggttct caaccggaac ctctccatct cggagaaccg
     2041 caccatgcag ctggcggtgc tggtcaatga ctcagacttc cagggcccag gagcgggcgt
     2101 cctcttgctc cacttcaacg tgtcggtgct gccggtcagc ctgcacctgc ccagtaccta
     2161 ctccctctcc gtgagcagga gggctcgccg atttgcccag atcgggaaag tctgtgtgga
     2221 aaactgccag gcattcagtg gcatcaacgt ccagtacaag ctgcattcct ctggtgccaa
     2281 ctgcagcacg ctaggggtgg tcacctcagc cgaggacacc tcggggatcc tgtttgtgaa
     2341 tgacaccaag gccctgcggc ggcccaagtg tgccgaactt cactacatgg tggtggccac
     2401 cgaccagcag acctctaggc aggcccaggc ccagctgctt gtaacagtgg aggggtcata
     2461 tgtggccgag gaggcgggct gccccctgtc ctgtgcagtc agcaagagac ggctggagtg
     2521 tgaggagtgt ggcggcctgg gctccccaac aggcaggtgt gagtggaggc aaggagatgg
     2581 caaagggatc accaggaact tctccacctg ctctcccagc accaagacct gccccgacgg
     2641 ccactgcgat gttgtggaga cccaagacat caacatttgc cctcaggact gcctccgggg
     2701 cagcattgtt gggggacacg agcctgggga gccccggggg attaaagctg gctatggcac
     2761 ctgcaactgc ttccctgagg aggagaagtg cttctgcgag cccgaagaca tccaggatcc
     2821 actgtgcgac gagctgtggc gcacggtgat cgcagccgct gtcctcttct ccttcatcgt
     2881 ctcggtgctg ctgtctgcct tctgcatcca ctgctaccac aagtttgccc acaagccacc
     2941 catctcctca gctgagatga ccttccggag gcccgcccag gccttcccgg tcagctactc
     3001 ctcttccagt gcccgccggc cctcgctgga ctccatggag aaccaggtct ccgtggatgc
     3061 cttcaagatc ctggaggatc caaagtggga attccctcgg aagaacttgg ttcttggaaa
     3121 aactctagga gaaggcgaat ttggaaaagt ggtcaaggca acggccttcc atctgaaagg
     3181 cagagcaggg tacaccacgg tggccgtgaa gatgctgaaa gagaacgcct ccccgagtga
     3241 gcttcgagac ctgctgtcag agttcaacgt cctgaagcag gtcaaccacc cacatgtcat
     3301 caaattgtat ggggcctgca gccaggatgg cccgctcctc ctcatcgtgg agtacgccaa
     3361 atacggctcc ctgcggggct tcctccgcga gagccgcaaa gtggggcctg gctacctggg
     3421 cagtggaggc agccgcaact ccagctccct ggaccacccg gatgagcggg ccctcaccat
     3481 gggcgacctc atctcatttg cctggcagat ctcacagggg atgcagtatc tggccgagat
     3541 gaagctcgtt catcgggact tggcagccag aaacatcctg gtagctgagg ggcggaagat
     3601 gaagatttcg gatttcggct tgtcccgaga tgtttatgaa gaggattcgt acgtgaagag
     3661 gagccagggt cggattccag ttaaatggat ggcaattgaa tccctttttg atcatatcta
     3721 caccacgcaa agtgatgtat ggtcttttgg tgtcctgctg tgggagatcg tgaccctagg
     3781 gggaaacccc tatcctggga ttcctcctga gcggctcttc aaccttctga agaccggcca
     3841 ccggatggag aggccagaca actgcagcga ggagatgtac tgcctgatgc tgcaatgctg
     3901 gaagcaggag ccggacaaaa ggccggtgtt tgcggacatc agcaaagacc tggagaagat
     3961 gatggttaag aggagagact acttggacct tgcggcgtcc actccatctg actccctgat
     4021 ttatgacgac ggcctctcag aggaggagac accgctggtg gactgtaata atgcccccct
     4081 ccctcgagcc ctcccttcca catggattga aaacaaactc tatggtagaa tttcccatgc
     4141 atttactaga ttcccaactt tcttgtacaa agtggttgct ctagaaggct ctggtaaacc
     4201 gattccgaac ccgctgctgg gtctggattc taccggttcc ggcaaaccga tcccgaaccc
     4261 gctgctgggc ctggactcta ccggttctgg tctagagggc ccgcggttcg aaggtaagcc
     4321 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     4381 ttgagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     4441 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4501 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4561 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4621 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4681 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4741 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4801 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4861 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4921 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4981 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     5041 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     5101 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     5161 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     5221 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5281 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5341 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     5401 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     5461 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     5521 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5581 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5641 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5701 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5761 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5821 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5881 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5941 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     6001 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     6061 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     6121 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     6181 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     6241 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     6301 gagttcttct gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct
     6361 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     6421 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     6481 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     6541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     6601 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     6661 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6721 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6781 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     6841 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     6901 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     6961 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7021 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7081 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7141 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7201 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7261 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7321 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7381 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7441 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7501 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7561 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7621 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7681 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7741 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7801 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7861 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7921 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     7981 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8041 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8101 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8161 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8221 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8281 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8341 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8401 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8461 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8521 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8581 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8641 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8701 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8761 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
