LOCUS       sequence-270487-         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein expression/crystallography.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  TITLE     Opher Gileadi SGC expression vectors
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBXTA-c022
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             871..915
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             916..933
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgtgcagca gccatcatca tcatcatcac ggcagcggca gcggctctga ccaggaggca
       61 aaaccttcaa ctgaggactt gggggataag aaggaaggtg aatatattaa actcaaagtc
      121 attggacagg atagcagtga gattcacttc aaagtgaaaa tgacaacaca tctcaagaaa
      181 ctcaaagaat catactgtca aagacagggt gttccaatga attcactcag gtttctcttt
      241 gagggtcaga gaattgctga taatcatact ccaaaagaac tgggaatgga ggaagaagat
      301 gtgattgaag tttaccagga gcaaacggga ggtggagaac tgcgtgtggg tctggaagaa
      361 agcgaactgt ggctgcgctt taaagagctg accaacgaaa tgatcgtgac caaaaacggc
      421 cgccgcatgt ttcctgtgct gaaagttaac gtgagcggcc tggacccgaa cgccatgtac
      481 agcttcctgc tggatttcgt tgccgcagat aaccaccgct ggaagtatgt gaacggcgaa
      541 tgggtgccgg gtggcaaacc ggaaccgcag gcacctagct gcgtgtatat ccacccggat
      601 agccctaact tcggtgccca ttggatgaaa gccccggtga gcttcagcaa ggtgaagctg
      661 accaacaaac tgaacggcgg cggccaaatc atgctgaaca gcttacataa atatgaacct
      721 cgcatccaca ttgtgcgtgt gggcgacccg caacgtatga tcaccagcca ttgcttcccg
      781 gagacacagt tcattgccgt taccgcctac cagaacgagg aaattaccgc actgaaaatt
      841 aaatacaacc cttttgcatg aagcggtggc ggtctgaacg acatcttcga ggctcagaaa
      901 atcgaatggc acgaacacca ccaccaccac cactga
//
