LOCUS       sequence-270576-       10023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of VN-fused and myc-tagged HLA-B*57:01.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10023)
  AUTHORS   McShan AC, Devlin CA, Overall SA, Park J, Toor JS, Moschidi D, 
            Flores-Solis D, Choi H, Tripathi S, Procko E, Sgourakis NG
  TITLE     Molecular determinants of chaperone interactions on MHC-I for 
            folding and antigen repertoire selection.
  JOURNAL   Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25602-25613. doi: 
            10.1073/pnas.1915562116. Epub 2019 Dec 3.
  PUBMED    31796585
REFERENCE   2  (bases 1 to 10023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1915562116"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2019-12-17- 17"; volume: "116"; issue: "51"; pages: 
            "25602-25613"
COMMENT     Sequence Label: pCEP4-myc-HLA-B57-VN
FEATURES             Location/Qualifiers
     source          1..10023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(721..739)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        807..911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(912..980,981..1772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1130..1149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1943..2531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2432..2451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2962..3107
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3150..4187
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4185..4204)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4229..4277
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4931..5310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5311..5514
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5465..5485
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5511..5535
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5617..5646
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(6694..6696,6697..7155)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYLTA"
     primer_bind     complement(6736..6757)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6997..7016)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    7178..7312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7202..7221)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7256..7275
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7455..7477)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8037..9826
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac
       61 gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg
      121 ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac
      181 cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct
      241 ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa
      301 gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg
      361 ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg
      421 tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg
      481 gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat
      541 tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc
      601 tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc
      661 tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg
      721 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      781 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      841 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      901 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      961 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1021 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1081 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1141 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1201 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1261 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1321 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1381 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1441 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1501 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1561 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1621 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1681 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1741 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1801 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1861 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1921 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1981 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2041 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     2101 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2161 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2281 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2341 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2401 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2461 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2521 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2581 gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca
     2641 tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg
     2701 cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct
     2761 ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct
     2821 ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc
     2881 tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat
     2941 ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat
     3001 tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg
     3061 acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc
     3121 gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg
     3181 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     3241 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     3301 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     3361 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     3421 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     3481 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     3541 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     3601 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     3661 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     3721 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     3781 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     3841 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     3901 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     3961 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     4021 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     4081 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     4141 gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag
     4201 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     4261 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     4321 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     4381 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     4441 agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg
     4501 ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac
     4561 ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg
     4621 cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc
     4681 caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg
     4741 ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat
     4801 tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca
     4861 ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga
     4921 ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     4981 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     5041 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     5101 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     5161 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
     5221 tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac
     5281 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
     5341 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     5401 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     5461 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     5521 gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat gaagaccatc
     5581 atcgccctga gctacatctt ctgcctggtg ttcgccgagc agaaattgat cagcgaggag
     5641 gacctgggca gccctggagg cagttcagga ggcggatctc attctatgag atatttctac
     5701 accgccatgt cccggcccgg ccgcggggag ccccgcttca tcgcagtggg ctacgtggac
     5761 gacacccagt tcgtgaggtt cgacagcgac gccgcgagtc cgaggatggc gccccgggcg
     5821 ccatggatag agcaggaggg gccggagtat tgggacgggg agacacggaa catgaaggcc
     5881 tccgcgcaga cttaccgaga gaacctgcgg atcgcgctcc gctactacaa ccagagcgag
     5941 gccgggtctc acatcatcca ggtgatgtat ggctgcgacg tggggccgga cgggcgcctc
     6001 ctccgcgggc atgaccagtc cgcctacgac ggcaaggatt acatcgccct gaacgaggac
     6061 ctgagctcct ggaccgcggc ggacacggcg gctcagatca cccagcgcaa gtgggaggcg
     6121 gcccgtgtgg cggagcagct gagagcctac ctggagggcc tgtgcgtgga gtggctccgc
     6181 agatacctgg agaacgggaa ggagacgctg cagcgcgcgg accccccaaa gacacatgtg
     6241 acccaccacc ccatctctga ccatgaggcc accctgaggt gctgggccct gggcttctac
     6301 cctgcggaga tcacactgac ctggcagcgg gatggcgagg accaaactca ggacaccgag
     6361 cttgtggaga ccagaccagc aggagataga accttccaga agtgggcagc tgtggtggtg
     6421 ccttctggag aagagcagag atacacatgc catgtacagc atgaggggct gccaaagccc
     6481 ctcaccctga gatgggagcc atcttcccaa tccactgttc ccatcgtcgg tatagttgct
     6541 ggcctcgcag tcttggcggt agtagtcatt ggagctgtag tggcagcagt gatgtgcaga
     6601 cgcaagagtt ccggcggcaa gggaggctcc tattctcaag ctgcttgctc cgactcagca
     6661 caaggcagtg atgtctcatt gactgcagga ggcgtgagca agggcgagga gctgttcacc
     6721 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     6781 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagct gatctgcacc
     6841 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgggcta cggcctgcag
     6901 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     6961 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     7021 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     7081 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     7141 gtctatctga ccgcctaact cgaggccggc aaggccggat ccagacatga taagatacat
     7201 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7261 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     7321 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     7381 gtaaaacctc tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt
     7441 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     7501 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     7561 ggcgcagcca tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga
     7621 ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt
     7681 acaacttgcc aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg
     7741 gttctctgac tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg
     7801 gctttcatcc tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt
     7861 gtaactcttg gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca
     7921 ggaagactac gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag
     7981 cggaccctca agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg
     8041 ggtagcatat gcttcccggg tagtagtata tactatccag actaacccta attcaatagc
     8101 atatgttacc caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg
     8161 aacagcgata tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc
     8221 cacgagggta gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca
     8281 gtgaactctc ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa
     8341 ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac
     8401 gcccccagaa taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata
     8461 taaccctcac aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat
     8521 ctttaacaat agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac
     8581 acgaatttat ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa
     8641 gatgtgtccc aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca
     8701 aggggaaaga gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg
     8761 aaaattaaac ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt
     8821 tttttgaaat tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg
     8881 actgtaaaat aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa
     8941 accacttgcc cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc
     9001 gggccaagat aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct
     9061 gagcgccaag cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg
     9121 ctaatgttgc catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa
     9181 tctatatctg ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa
     9241 tctatatctg ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa
     9301 tctatatctg ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa
     9361 tctgtatccg ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa
     9421 tttatatctg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     9481 tatctgggta gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta
     9541 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta
     9601 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     9661 tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca
     9721 tatacagtca gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg
     9781 ccacctccca agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat
     9841 tcttaggtga atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt
     9901 aaatgtcgct aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac
     9961 aacatgggta tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg
    10021 cca
//
