LOCUS       sequence-270696-        4539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  entry vector for GreenGate cloning method, C module, open reading 
            frame of phosphatase-inactive PROPYZAMIDE-HYPERSENSITIVE 1 (PHS1) 
            (PHS1DP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Vilches Barro A, Stockle D, Thellmann M, Ruiz-Duarte P, Bald L, 
            Louveaux M, von Born P, Denninger P, Goh T, Fukaki H, Vermeer JEM, 
            Maizel A
  TITLE     Cytoskeleton Dynamics Are Necessary for Early Events of Lateral Root
            Initiation in Arabidopsis.
  JOURNAL   Curr Biol. 2019 Aug 5;29(15):2443-2454.e5. doi: 
            10.1016/j.cub.2019.06.039. Epub 2019 Jul 18.
  PUBMED    31327713
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2019.06.039"; journalName: "Curr Biol"; date: 
            "2019-08-5- 5"; volume: "29"; issue: "15"; pages: "2443-2454.e5"
COMMENT     Sequence Label: pLB038 - PHS1∆P
FEATURES             Location/Qualifiers
     source          1..4539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2144..2149,2150..2167,2168..2174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        2212..2230
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2212..2229
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4125..4144)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4126..4144)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4154..4176)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4370..4389)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4489..4511
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaggc tcaacaatgg tcactagtgc aggagagagg
     2281 aatactgatc ctaaaagtcc accaagtagg cagagttcag agattagttt gtgggaaagg
     2341 cttggtaaag cttctactgt ggatattgat tcgagctgtt tttcatggaa tatgttgtct
     2401 tcacttcatc atactgaaca tagtagtagc actgatcatt ctgaagaaga ccagagtaaa
     2461 ccacttgagg ttactgtgaa ttctggagga gttgttttct ttgccctgtt caatagttcc
     2521 agctctgaag atgcctctcg taaggaagaa gcagcagtta ttaaatttgc atcatcgagg
     2581 atggccacgc aatcagaacg tctaggttat gagttttcca agtggctagg ggtgcagatt
     2641 ccgcaggcta gagtaattca cagctgtaat cctgagtgga cactgatcaa agaagccact
     2701 gaaaaagcac aagctaaagc gacttcggag ggggacgagg ttggtgaaat gacatgttca
     2761 gaacttctgg aagcccttga gctcagccgt tgtcttctct taatgagtta tgttcatgga
     2821 tgtccaatgc tggagagcat gagtagtttt gaaactgaag aaaaagcaga gagagctgct
     2881 gcagctctag ggagaatctt ggtgttagat ctagtaatta ggaatgaaga cagattaccg
     2941 tgtcgtcagc tgagatggcg tggcaacccg gcaaatctgt tactgacaga cagaattgtg
     3001 tcttctgcaa agcatcatga atgttcattt gatgaagctt ttgattccgc catcaaacgg
     3061 taccatccaa aggactatag atccattcaa agggaaagaa gagcttcttc cgtagatagc
     3121 agatccaggt tgagtatcag tgatcaaatg ttagtctcac aggcttctga tttttctgat
     3181 ataacggaat cacctagatc atatgatact ggtttaatga gtccaatgtc tgatagatca
     3241 gtagctgccg attttcacct cgttgccata gactcgggtg ttccacggcg gcctcctgct
     3301 ggaaaacggg caagcgacca agaaatttat ccaagattag ttgagttact cctcaatagt
     3361 agccaatatt cttcgaattt gttacatgaa ataacagaag gaagtttagg atatccacag
     3421 gcagaagatg gtgaagaaac atctaatgtt cgatctgtag taacaccagt tgtacgcgag
     3481 tttcgaaatg gtttccgagc tggtcttagg gatttgcaag agtttcacat cttccttgtc
     3541 acccttcatc agaaattaga tgtgttgctt cgggcatttt ttagtatgat ggacaaaaca
     3601 atgtgtgcag attttgacag agaagacttt gctgttcctg agtcaccttc acatactcat
     3661 gggcatgagg tgaatcatta tccatctccc tcaaaagatc gggtgcctag tgataacagc
     3721 tctgatcaca gtgagtcaga tatgcaaaaa tcagttccta ggacaccaaa ttcagaaaac
     3781 aaagaagacg ggtcttcgcc taagtcacgt gaaagttggc acggaagatc cggtaaaggt
     3841 ggagaatctc ttagcagtca gcgtttggca gcaaagctcc gtgacttcca taagtttgca
     3901 aaggttgatg ctgagtctaa taaagaacta gaccagtgga atgaaacact gcggaatgaa
     3961 gtgatgaaac tttgccaaga gaacggtttc aacactggtt tttttgaggg cagtgataat
     4021 aacagttgca cagatgctta tgagctaaag gttagacttg agcatattct tgagaggata
     4081 tcattgatta gcaaagctgc taattcagtg agaccgaatt ctcgccctat agtgagtcgt
     4141 attacctggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     4201 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     4261 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     4321 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     4381 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4441 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4501 tgtgtcagag gttttcaccg tcatcaccga aacgcgcga
//
