LOCUS       sequence-270740-       11124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral inducible expression (TetOn) of human ECT2 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11124)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 11124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLENTI_TetOn_Hu_ECT2(WT)
FEATURES             Location/Qualifiers
     source          1..11124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1941..2255
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1949..1967
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1985..2003
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2020..2038
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2056..2074
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2092..2110
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2127..2145
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2163..2181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2228..2252
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2333..2357
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5040..5064)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5095..5683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5148..5168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5566..5577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5739..6226
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5774..5797)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6159..6178
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6244..7269
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6244..7269
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7643..7876
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7948..8069
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7985..8004)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8039..8058
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8109..8244
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8264..8283)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8265..8283)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8293..8310)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8293..8309)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8302..8324)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8451..8906
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8538..8557)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8748..8769
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8932..9036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9037..9105,9106..9897)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9255..9274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10068..10656
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10557..10576
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10810..10827
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10944..10965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10980..10985,10986..11003,11004..11010)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11018..11034
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11023..11045
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11042..11058
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11042..11058
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11077..11097
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11079..11097
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcacga gactagcctc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     1981 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     2041 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     2101 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     2161 actccctatc agtgatagag aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata
     2221 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag aattcgagct cggtacccgg
     2281 ggatccaccg gttcgtcgac tagtccagtg tggtggaatt ctgcagatat caacaagttt
     2341 gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg gctgaaaata gtgtattaac
     2401 atccactact gggaggacta gcttggcaga ctcttccatt tttgattcta aagttactga
     2461 gatttccaag gaaaacttac ttattggatc tacttcatat gtagaagaga tgcctcagat
     2521 tgaaacaaga gtgatattgg ttcaagaagc tggaaaacaa gaagaactta taaaagcctt
     2581 aaaggacatt aaagtgggct ttgtaaagat ggagtcagtg gaagaatttg aaggtttgga
     2641 ttctccggaa tttgaaaatg tatttgtagt cacggacttt caggattctg tctttaatga
     2701 cctctacaag gctgattgta gagttattgg accacccgtg gtgctgaatt gttcacaaaa
     2761 aggagagcct ttgccatttt catgtcgccc gttgtattgt acaagtatga tgaatctagt
     2821 actatgcttt actggattta ggaaaaaaga agaactagtc aggttggtga cattggtcca
     2881 tcacatgggt ggagttattc gaaaagactt taattcaaaa gttacacatt tggtggcaaa
     2941 ttgtacacaa ggagaaaaat tcagggttgc tgtgagtcta ggtactccaa ttatgaagcc
     3001 agaatggatt tataaagctt gggaaaggcg gaatgaacag gatttctatg cagcagttga
     3061 tgactttaga aatgaattta aagttcctcc atttcaagat tgtattttaa gtttcctggg
     3121 attttcagat gaagagaaaa ccaatatgga agaaatgact gaaatgcaag gaggtaaata
     3181 tttaccgctt ggagatgaaa gatgcactca ccttgtagtt gaagagaata tagtaaaaga
     3241 tcttcccttt gaaccttcaa agaaacttta tgttgtcaag caagagtggt tctggggaag
     3301 cattcaaatg gatgcccgag ctggagaaac tatgtattta tatgaaaagg caaatactcc
     3361 tgagctcaag aaatcagtgt caatgctttc tctaaatacc cctaacagca atcgcaaacg
     3421 acgtcgttta aaagaaacac ttgctcagct ttcaagagag acagacgtgt caccatttcc
     3481 accccgtaag cgcccatcag ctgagcattc cctttccata gggtcactcc tagatatctc
     3541 caacacacca gagtctagca ttaactatgg agacacccca aagtcttgta ctaagtcttc
     3601 taaaagctcc actccagttc cttcaaagca gtcagcaagg tggcaagttg caaaagagct
     3661 ttatcaaact gaaagtaatt atgttaatat attggcaaca attattcagt tatttcaagt
     3721 accattggaa gaggaaggac aacgtggtgg acctatcctt gcaccagagg agattaagac
     3781 tatttttggt agcatcccag atatctttga tgtacacact aagataaagg atgatcttga
     3841 agaccttata gttaattggg atgagagcaa aagcattggt gacatttttc tgaaatattc
     3901 aaaagatttg gtaaaaacct accctccctt tgtaaacttc tttgaaatga gcaaggaaac
     3961 aattattaaa tgtgaaaaac agaaaccaag atttcatgct tttctcaaga taaaccaagc
     4021 aaaaccagaa tgtggacggc agagccttgt tgaacttctt atccgaccag tacagaggtt
     4081 acccagtgtt gcattacttt taaatgatct taagaagcat acagctgatg aaaatccaga
     4141 caaaagcact ttagaaaaag ctattggatc actgaaggaa gtaatgacgc atattaatga
     4201 ggataagaga aaaacagaag ctcaaaagca aatttttgat gttgtttatg aagtagatgg
     4261 atgcccagct aatcttttat cttctcaccg aagcttagta cagcgggttg aaacaatttc
     4321 tctaggtgag cacccctgtg acagaggaga acaagtaact ctcttcctct tcaatgattg
     4381 cctagagata gcaagaaaac ggcacaaggt tattggcact tttaggagtc ctcatggcca
     4441 aacccgaccc ccagcttctc ttaagcatat tcacctaatg cctctttctc agattaagaa
     4501 ggtattggac ataagagaga cagaagattg ccataatgct tttgccttgc ttgtgaggcc
     4561 accaacagag caggcaaatg tgctactcag tttccagatg acatcagatg aacttccaaa
     4621 agaaaactgg ctaaagatgc tgtgtcgaca tgtagctaac accatttgta aagcagatgc
     4681 tgagaatctt atttatactg ctgatccaga atcctttgaa gtaaatacaa aagatatgga
     4741 cagtacattg agtagagcat caagagcaat aaaaaagact tcaaaaaagg ttacaagagc
     4801 attctctttc tccaaaactc caaaaagagc tcttcgaagg gctcttatga catcccacgg
     4861 ctcagtggag ggaagaagtc cttccagcaa tgataagcat gtaatgagtc gtctttctag
     4921 cacatcatca ttagcaggta tcccttctcc ctcccttgtc agccttcctt ccttctttga
     4981 aaggagaagt catacgttaa gtagatctac aactcatttg ataaagggtg ggcgcgccga
     5041 cccagctttc ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa
     5101 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     5161 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     5221 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     5281 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     5341 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     5401 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     5461 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     5521 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     5581 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5641 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata
     5701 tccggactag tgatctctcg aggttaacga attctaccgg gtaggggagg cgcttttccc
     5761 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
     5821 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
     5881 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
     5941 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
     6001 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
     6061 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
     6121 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     6181 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatcccgcc
     6241 accatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     6301 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     6361 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     6421 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     6481 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     6541 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     6601 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     6661 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     6721 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     6781 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     6841 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     6901 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     6961 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     7021 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     7081 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     7141 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     7201 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     7261 aaggaataga gtagatgccg accgaacaag agctgatttc gagaacgcct cagccagcaa
     7321 ctcgcgcgag cctagcaagg caaatgcgag agaacggcct tacgcttggt ggcacagttc
     7381 tcgtccacag ttcgctaagc tcgctcggct gggtcgcggg agggccggtc gcagtgattc
     7441 aggcccttct ggattgtgtt ggtccccagg gcacgattgt catgcccacg cactcgggtg
     7501 atctgactga tcccgcagat tggagatcgc cgcccgtgcc tgccgattgg gtgcagatcc
     7561 gtcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7621 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     7681 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7861 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     7921 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     7981 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8041 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     8101 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     8161 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     8221 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     8281 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     8341 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     8401 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     8461 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     8521 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     8581 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     8641 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     8701 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     8761 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     8821 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     8881 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     8941 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9001 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9241 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     9301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9541 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     9781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     9841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     9901 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     9961 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    10021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    10081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    10141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    10201 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    10261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    10321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    10381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    10441 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    10501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    10561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    10621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
    10681 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
    10741 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
    10801 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
    10861 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
    10921 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
    10981 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
    11041 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
    11101 caaaagctgg agctgcaagc ttaa
//
