LOCUS       sequence-270814-        3195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human MAD2 cDNA in Gateway pENTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3195)
  TITLE     de Carcer unpublished plasmids
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pENTR_Hu MAD2(WT)
FEATURES             Location/Qualifiers
     source          1..3195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(752..851)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(868..887)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(869..887)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(892..908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(892..908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1010..1029)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1021..1830
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1088..1107)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1977..2565
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2466..2485
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2719..2736
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2895..2922)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3014..3100)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3149..3171
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3163..3180
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3164..3180
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcgctgc agctctcccg ggagcaggga atcaccctgc gcgggagcgc cgaaatcgtg
      181 gccgagttct tctcattcgg catcaacagc attttatatc agcgtggcat atatccatct
      241 gaaaccttta ctcgagtgca gaaatacgga ctcaccttgc ttgtaactac tgatcttgag
      301 ctcataaaat acctaaataa tgtggtggaa caactgaaag attggttata caagtgttca
      361 gttcagaaac tggttgtagt tatctcaaat attgaaagtg gtgaggtcct ggaaagatgg
      421 cagtttgata ttgagtgtga caagactgca aaagatgaca gtgcacccag agaaaagtct
      481 cagaaagcta tccaggatga aatccgttca gtgatcagac agatcacagc tacggtgaca
      541 tttctgccac tgttggaagt ttcttgttca tttgatctgc tgatttatac agacaaagat
      601 ttggttgtac ctgaaaaatg ggaagagtcg ggaccacagt ttattaccaa ttctgaggaa
      661 gtccgccttc gttcatttac tactacaatc cacaaagtaa atagcatggt ggcctacaaa
      721 attcctgtca atgacaaggg tgggcgcgcc gacccagctt tcttgtacaa agttggcatt
      781 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      841 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
      901 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
      961 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     1021 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     1081 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     1141 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     1201 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     1261 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     1321 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     1381 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1441 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1501 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1561 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1621 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1681 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1741 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1801 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     1861 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     1921 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1981 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     2041 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     2101 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     2161 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     2221 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     2281 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     2341 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     2401 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     2461 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2521 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2581 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2641 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2701 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     2761 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     2821 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     2881 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     2941 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     3001 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     3061 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     3121 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     3181 cttaagctcg ggccc
//
