LOCUS       sequence-270980-        6628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transcriptional GFP reporter of acr-2 (cholinergic) expressing 
            neurons in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Jospin M, Qi YB, Stawicki TM, Boulin T, Schuske KR, Horvitz HR, 
            Bessereau JL, Jorgensen EM, Jin Y
  TITLE     A neuronal acetylcholine receptor regulates the balance of muscle 
            excitation and inhibition in Caenorhabditis elegans.
  JOURNAL   PLoS Biol. 2009 Dec;7(12):e1000265. doi: 
            10.1371/journal.pbio.1000265. Epub 2009 Dec 22.
  PUBMED    20027209
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2009-12"; volume: "7(12):e1000265. doi"; pages: " 
            10.1371/journal.pbio.1000265. Epub 2009 Dec 22"
COMMENT     Sequence Label: pCZGY921
FEATURES             Location/Qualifiers
     source          1..6628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..56)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(40..56)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(793..813)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(793..811)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(832..848)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(832..848)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(845..867)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    856..872
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(880..886,887..904,905..910))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    925..946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1063..1080)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1234..1822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1314..1333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1993..2784,2785..2853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2616..2635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2854..2958)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2984..3439)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3121..3142)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3333..3352
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3566..3588
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3580..3597
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3581..3597
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3607..3626
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3607..3625
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5673..5697
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5764..5792)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6540..6560
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(join(6618..6628,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggtgatatc gaattcctgc agcccggggg atccactagt tctagagcgg
       61 ccgccaccgc ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag
      121 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
      181 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
      241 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
      301 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
      361 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
      421 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
      481 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
      541 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
      601 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
      661 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
      721 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccgagctc
      781 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
      841 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      901 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      961 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     1021 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1081 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1141 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     1681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1921 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1981 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2041 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2101 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2161 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2221 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2281 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2341 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2401 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2461 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2521 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2581 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2641 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2701 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2761 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2821 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2881 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2941 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     3001 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     3061 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     3121 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     3181 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     3241 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     3301 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     3361 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     3421 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3481 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3541 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     3601 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ttttcagctc accaccgact
     3661 gattttcatt tcaaaattaa tgcagatttc tacgtgttct ttttcaaata aatttatata
     3721 ttttttgttc tctcacaaaa taaataattt cgagagttaa gtgatcattc tcagacatat
     3781 tctataattg tccgtcagga aacagataga ggaacctttg ataaataaat tataaaaggc
     3841 cgacagggaa atgcttctcc ctcgactctg ttagaagcga agaggcaatt tttaaaaatt
     3901 tcccattttt atgcgttttt ctgatttcca aagtcacgca tcatttacca accgcaacga
     3961 ttcataagga cgtaattttg attttctgga cgatttttat atagttttga tgtgaaattc
     4021 gctttatttg tctgaaagtg gaatagattt agtgtggatt taaccgaaaa atgcagatct
     4081 tttaaaaatt taaaccagtg ttttgttgcc ttatttgcga aaaaaaattg tgatatttcg
     4141 agtattgctg caatttgatt agtaaaatgt taggatataa gtatttaaaa agtataattg
     4201 gttgaatttt ctttttcatc accgacttcc aaaaatatag agatactgag ttttcaccat
     4261 tttttgaatg taaataaaaa cattttgaac acttgtgctg ggattcaatc caaatacatt
     4321 cactgtcatt taatattatt atatatcaaa ataaaacgca taactttttc tgtttttttt
     4381 taaatttttt caaaatgtag gtttggtaag ttaaggtaag gttaggcaat gtaggtaaac
     4441 acgagatgat actccacgtt gaaaattgtt ttcataaata cagtttagat tgaactgaag
     4501 gattttatcg tctaatagaa tataattttt tagttcaaga tttaggattt gttttcactt
     4561 ttttttagat tcaagacata tatctcgcaa aatgagtttt gtctttgagc catcttgtca
     4621 tcatagcaca ttgaattttg ttgttcgcaa gacggaatat atgttcccag ctgcaaattt
     4681 tgtgtcggat atttcttctt tagcgaaagg cgttaaaaac caaaaatagt cgaagcgttt
     4741 gatatgtgtc ccgttggttt ttcgtgtcca tctattctgt ttcgagtaca catgttgcga
     4801 cgcggcactg atgaaaaagt gaaagagcta gtgtcacaca caaacacaaa gtatgctcat
     4861 gtgagtgtgt agatatgtgt agatgtatgt cctgaaggaa taaaaagact ttttgaagct
     4921 acatttttaa acaaaaaaag acagaaacat gaagcacaac tctctattct gtcgaacggc
     4981 gtgtgcaact cacgcacacc caaaaatatg aaatataaca ttcgcaccaa caaagcgcga
     5041 atatcaatga atctcgaggg aacgatggct gcctgccggc gcgtgttgct cttctgtcat
     5101 tgggataact ggcgggtctg ttgggtccgt cctttagacc atttgactac actgccctgc
     5161 atatgtgtga aacgtcgtcg ttgctgttgg gagctgtctg tgtgtatgtg tgtgtgtgtg
     5221 agtatggagc agtaaaaagc tgccgatacg agaagcgccg ccccggacgg acggcgatgg
     5281 cgcatcgcca aatgtgtcat tttagttgtc ttcacgaacc agagggccca cgacggacgg
     5341 gcggccacgc ccacctcaac cagaatatcc ttccttctcc cacccaccct tgcggacgcg
     5401 cggatgcgct gaacaaaaag acgacgaaga agaagaagaa gaagctgaac gaagaaaaaa
     5461 gcatcaaaag aagtgaagcg gctctaccac cccgaccacc ttccttcaca ggaaggacgc
     5521 cgttttcaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat tggccaaagg
     5581 acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag gaggaccctt
     5641 ggagggtacc ggtagaaaaa aagcttgata tcacaagttt gtacaaaaaa gcaggctccg
     5701 aattcgccct tcgacgtgtt accggtagaa aaaatgagta aaggagaaga acttttcact
     5761 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
     5821 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
     5881 actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat
     5941 tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg
     6001 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
     6061 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa
     6121 cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa
     6181 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     6241 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
     6301 aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac taactaatct
     6361 gatttaaatt ttcagaactt caaaattaga cacaacattg aagatggaag cgttcaacta
     6421 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     6481 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     6541 gtccttcttg agtttgtaac agctgctggg attacacatg gcatggacga actatacaaa
     6601 taacaagggc gaattcgacc cagctttc
//
