LOCUS       sequence-271069-         519 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) 
            suppression; for transient or stable piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMma PylT(UUA)_FLAG-AcKRS
FEATURES             Location/Qualifiers
     source          1..519
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
       61 ggggaaaagg cctccacggc caactagttc tgcagtattt agcatgcccc acccatctgc
      121 aaggcattct ggatagtgtc aaaacagccg gaaatcaagt ccgtttatct caaactttag
      181 cattttggga ataaatgata tttgctatgc tggttaaatt agattttagt taaatttcct
      241 gctgaagctc tagtacgata agtaacttga cctaagtgta aagttgagat ttccttcagg
      301 tttatatagc ttgtgcgccg cctgggtacc tcggaaacct gatcatgtag atcgaacgga
      361 ctttaaatcc gttcagccgg gttagattcc cggggtttcc ggacaagtgc ggtttttcct
      421 gcagtattta gcatgcccca cccatctgca aggcattctg gatagtgtca aaacagccgg
      481 aaatcaagtc cgtttatctc aaactttagc attttggga
//
