LOCUS       sequence-271080-         360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter 
            sfGFP 150 TAG stop; for transient transfection or stable 
            piggyBac-mediated integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
  TITLE     Methanomethylophilus alvus Mx1201 provides basis for mutual 
            orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in 
            mammalian cells.
  JOURNAL   ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571.
  PUBMED    30260624
REFERENCE   2  (bases 1 to 360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571."
COMMENT     Sequence Label: pAS_4xMx PylT C41CA_GFP 150TAG
FEATURES             Location/Qualifiers
     source          1..360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcctttctag agcactagtg gccttggagc tgcagtattt agcatgcccc acccatctgc
       61 aaggcattct ggatagtgtc aaaacagccg gaaatcaagt ccgtttatct caaactttag
      121 cattttggga ataaatgata tttgctatgc tggttaaatt agattttagt taaatttcct
      181 gctgaagctc tagtacgata agtaacttga cctaagtgta aagttgagat ttccttcagg
      241 tttatatagc ttgtgcgccg cctgggtacc tcgggggacg gtccggcgac cagcgggtct
      301 ctaaaaccta gccaagcggg gttcgacgcc ccggtctctc ggacaagtgc ggtttttatc
//
