LOCUS       sequence-27117-e         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Jho EH, Zhang T, Domon C, Joo CK, Freund JN, Costantini F
  TITLE     Wnt/beta-catenin/Tcf signaling induces the transcription of Axin2, a
            negative regulator of the signaling pathway.
  JOURNAL   Mol Cell Biol. 2002 Feb . 22(4):1172-83.
  PUBMED    11809808
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2002 Feb . 22(4):1172-83."
COMMENT     Sequence Label: pd2EGFP-1
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    24..101
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(307..326)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcccttgct caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
       61 gaattcgaag cttgagctcg agatctgagt ccggtagcgc tagtaataac taatgcatgg
      121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      721 ttggtagctc ttgatccggc aaacaaacca ccgctggaac ggggtttttt ttgtt
//
