LOCUS       sequence-271195-        2884 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Low phosphate inducible mCherry with N-terminal linker and His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Menacho-Melgar R, Ye Z, Moreb EA, Yang T, Efromson JP, Decker JS, 
            Wang R, Lynch MD
  TITLE     Scalable, two-stage, autoinduction of recombinant protein expression
            in E. coli utilizing phosphate depletion.
  JOURNAL   Biotechnol Bioeng. 2020 May 22. doi: 10.1002/bit.27440.
  PUBMED    32441815
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2020 May 22. doi: 10.1002/bit.27440."
COMMENT     Sequence Label: pHCKan-yibDp-mCherry
FEATURES             Location/Qualifiers
     source          1..2884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1021..1067
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1223..1254
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        1255..1357
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1358..2173
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1431..1450)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2194..2221
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(2233..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2313..2332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2842..2871)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 cagtccagtt acgctggagt ctgaggctcg tcctgaatga tatcaagctt gaattcgttc
       61 gtgcgtaatt gtgctgatct cttatatagc tgctctcatt atctctctac cctgaagtga
      121 ctctctcacc tgtaaaaata atatctcaca ggcttaatag tttcttaata caaagcctgt
      181 aaaacgtcag gataacttct gtgtaggagg ataatctatg gtatcaaaag gagaggaaga
      241 taacatggct attatcaaag aatttatgcg cttcaaagtt cacatggaag gtagcgtgaa
      301 cggtcacgag ttcgagattg aaggtgaagg tgaagggcgt ccgtacgaag gtacacagac
      361 cgctaaactg aaggtgacga aaggtggccc tcttccattt gcgtgggata ttcttagtcc
      421 gcaatttatg tatggatcta aggcgtatgt caagcacccg gctgacatcc cagattactt
      481 aaaacttagc ttcccagagg gattcaaatg ggagcgcgtt atgaatttcg aggacggcgg
      541 tgtagtgacc gtcactcagg attcatcact tcaagatggc gaatttatct acaaggtcaa
      601 gctgcgtggg acaaattttc cgtcggatgg gcctgtcatg cagaagaaga caatgggctg
      661 ggaagcgtcg tcagagcgta tgtatccaga ggacggagcg ttaaaagggg aaattaagca
      721 gcgcctgaag ttgaaggatg gcgggcatta tgacgcagag gtaaaaacca cttataaagc
      781 gaaaaagcca gtccaattgc caggagccta caatgtcaat atcaaattag atatcacaag
      841 tcataacgag gattacacga tcgtcgaaca atatgagcgc gcagaaggtc gccatagtac
      901 aggaggaatg gacgaactgt acaaataatg actcgagtct ggtaaaacta gcattcgacc
      961 tagcataacc ccgcggggcc tcttcggggg tctcgcgggg ttttttgctg aaagaagctt
     1021 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1081 ctgcggccgg gtcaggtatg atttaaatgg tcagtaacgg gtcttgaggg gttttttgca
     1141 atgggttcat cccgtgggga cgaattctct agatatcgct caatactgac catttaaatc
     1201 atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg
     1261 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     1321 tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac
     1381 gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     1441 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
     1501 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     1561 gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     1621 ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca
     1681 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     1741 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg
     1801 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga
     1861 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt
     1921 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     1981 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     2041 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     2101 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga
     2161 tgagtttttc tgagggccca aatgtaatca cctggctcac cttcgggtgg gcctttctgc
     2221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgatgctc
     2281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2521 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2701 gaagccagtt acctcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgattttcta ccgaagaaag gcccacccgt gaaggtgagc cagtgagttg
     2881 attg
//
