LOCUS       sequence-27129-e         560 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Tritschler F, Eulalio A, Truffault V, Hartmann MD, Helms S, Schmidt 
            S, Coles M, Izaurralde E, Weichenrieder O
  TITLE     A divergent Sm fold in EDC3 proteins mediates DCP1 binding and 
            P-body targeting.
  JOURNAL   Mol Cell Biol. 2007 Dec . 27(24):8600-11.
  PUBMED    17923697
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Dec . 27(24):8600-11."
COMMENT     Sequence Label: pAc5.1B-EGFP
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..57)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(169..190)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     518..537
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atgatgaccg gtacgcgtag aatcgagacc gaggagaggg ttagggatag gcttaccttc
       61 gaaccgcggg ccctctagac tcgagcggcc gccactgtgc tggatatctg cagaattcaa
      121 gcttggcttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      181 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact gggtgctcag
      241 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      301 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      361 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      421 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      481 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      541 gaagaagatg gtgcgctcct
//
