LOCUS       sequence-27135-e         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E
  TITLE     A crucial role for GW182 and the DCP1:DCP2 decapping complex in 
            miRNA-mediated gene silencing.
  JOURNAL   RNA. 2005 Nov . 11(11):1640-7.
  PUBMED    16177138
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2005 
            Nov . 11(11):1640-7."
COMMENT     Sequence Label: pAc5.1C-FLuc-Stop-5BoxB
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    138..156
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    180..198
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    204..222
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    262..280
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    286..304
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     CDS             340..381
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             391..408
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(431..448)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    481..615
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(505..524)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     559..578
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gagtccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctnataaa
       61 ggccaagaag ggcggaaagt ccaaattgta agaattcgct agcttcccta agtccaacta
      121 ccaaactggg gattcctggg ccctgaagaa gggcccctcg actagtccaa atacaaactg
      181 ggccctgaag aagggcccat atagggccct gaagaagggc cctatcgagg atattatctc
      241 gactaagtcc aactacaaac tgggccctga agaagggccc atatagggcc ctgaagaagg
      301 gccctatcga ggatattatc tcgaggtcac ccattcgaag gtaagcctat ccctaaccct
      361 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
      421 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
      481 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
      541 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      601 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
      661 gtgtgggagg ttttttaaag caagtaaanc tctacaaatg tggtatggct gattatgatc
      721 agtc
//
