LOCUS       sequence-27136-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Rehwinkel J, Behm-Ansmant I, Gatfield D, Izaurralde E
  TITLE     A crucial role for GW182 and the DCP1:DCP2 decapping complex in 
            miRNA-mediated gene silencing.
  JOURNAL   RNA. 2005 Nov . 11(11):1640-7.
  PUBMED    16177138
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2005 
            Nov . 11(11):1640-7."
COMMENT     Sequence Label: pAc5.1C-FLuc-Stop-5BoxB
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..62)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    98..116
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    122..140
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    180..198
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    204..222
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
     protein_bind    246..264
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 aggtgatgat gaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
       61 ccttcgaatg ggtgacctcg agataatatc ctcgataggg cccttcttca gggccctata
      121 tgggcccttc ttcagggccc agtttgtagt tggacttagt cgagataata tcctcgatag
      181 ggcccttctt cagggcccta tatgggccct tcttcagggc ccagtttgta tttggactag
      241 tcgaggggcc cttcttcagg gcccaggaat ccccagtttg gtagttggac ttagggaagc
      301 tagcgaattc ttacaatttg gactttccgc ccttcttggc ctttatgagg atctctctga
      361 tttttcttgc gtcgagtttt ccggtaagac ctttcggtac ttcgtccaca aacacaactc
      421 ctccgcgcaa ctttttcgcg gttgttactt gactggcgac gtaatccacg atctcttttt
      481 ccgtcatcgt ctttccgtgc tccaaaacaa caacggcggc gggaagttca ccggcgtcat
      541 cgtcgggaag acctgccacg cccgcgtcga agatgttggg gtgttgtaac aatatcgatt
      601 ccaattcagc gggggccacc tgataccctt tgtatttaat taaagacttc aagcggtcaa
      661 ctatgaagaa gtgttc
//
