LOCUS       sequence-271382-         659 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used to sense miRNA activity using fluorescence based tools. 
            hsa-miR-374b-5p target in 3' UTR of of mKate. miRNA activity can be 
            measured by the repression of mKate2 relative to EBFP2 fluorescent 
            proteins . Plasmid is inherently unstable; please see Depositor 
            Comments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Gam JJ, Babb J, Weiss R
  TITLE     A mixed antagonistic/synergistic miRNA repression model enables 
            accurate predictions of multi-input miRNA sensor activity.
  JOURNAL   Nat Commun. 2018 Jun 22;9(1):2430. doi: 10.1038/s41467-018-04575-0.
  PUBMED    29934631
REFERENCE   2  (bases 1 to 659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-06-22"; volume: "9(1):2430. doi"; pages: " 
            10.1038/s41467-018-04575-0"
COMMENT     Sequence Label: LSB-hsa-miR-374b-5p
FEATURES             Location/Qualifiers
     source          1..659
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..132)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(356..375)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    421..476
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(475..494)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 caaggaggcc gacaaagaga catacgtcga gcagcacgag gtggctgtgg ccagatactg
       61 cgacctccct agcaaactgg ggcacaaact taattgagaa ttctaccacc cagctttctt
      121 gtacaaagtg gtacgtctca agaccagagg tctctgcttg atactgacac ttagcaggtt
      181 gtattatatc acttagcagg ttgtattata tcacttagca ggttgtatta tatcacttag
      241 caggttgtat tatatcaacc agaagagacc tgtgcttcac tcctcaggtg caggctgcct
      301 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt
      361 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
      421 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
      481 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
      541 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
      601 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttga
//
