LOCUS       sequence-271774-       12546 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd generation lentiviral expression of Flag-tagged Casilio-ME3.1 
            effector hNEIL2-PUFa-hTET1(CD) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12546)
  AUTHORS   Taghbalout A, Du M, Jillette N, Rosikiewicz W, Rath A, Heinen CD, Li
            S, Cheng AW
  TITLE     Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated 
            RNA-guided coupling of methylcytosine oxidation and DNA repair 
            pathways.
  JOURNAL   Nat Commun. 2019 Sep 20;10(1):4296. doi: 10.1038/s41467-019-12339-7.
  PUBMED    31541098
REFERENCE   2  (bases 1 to 12546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-09-20"; volume: "10(1):4296. doi"; pages: " 
            10.1038/s41467-019-12339-7"
COMMENT     Sequence Label: pAT608_CMV/CAG_Flag-hNEIL2-PUFa-hTET1(CD)
FEATURES             Location/Qualifiers
     source          1..12546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2767..3267
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3186..3204
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3289..3888
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3289..3308)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3785..3805
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3930..4047
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4164..4467
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4468..4671
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4622..4642
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4694..4718
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4736..4745
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4745..4768
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5822..5842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5846..5866
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5870..5890
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5981..6001
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7058..7078
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7082..7102
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(9347..9371)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9373..9414
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9456..10044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9509..9529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9927..9938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10116..10349
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10427..10548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10464..10483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10518..10537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10588..10723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10743..10762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10744..10762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10772..10789)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10772..10788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10781..10803)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10930..11385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11017..11036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11227..11248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11411..11515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11516..11584,11585..12376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11734..11753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     2821 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     2881 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     2941 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     3001 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     3061 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     3121 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     3181 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     3241 gaccgaatca ccgacctctc tccccagggg gtaccaccgg agcttaccat gaccgagtac
     3301 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     3361 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     3421 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     3481 tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag cgtcgaagcg
     3541 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     3601 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     3661 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     3721 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     3781 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     3841 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaga attctagatc
     3901 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     3961 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4021 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
     4081 tggcgccggc tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt
     4141 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     4201 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     4261 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     4321 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     4381 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     4441 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     4501 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     4561 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     4621 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     4681 actgtcggga tcaacaagtt tgtacaaaaa agcaggctcc gaattcaccg gtgccgccac
     4741 catggactac aaggacgacg atgacaagcc caagaaaaag cgcaagttgc cagaagggcc
     4801 gttggtgagg aaatttcacc atttggtctc cccctttgtg ggtcagcagg tggtcaagac
     4861 agggggcagc agtaagaagc tacagcccgc cagcctgcag tctctgtggc tccaggacac
     4921 ccaggtccat ggaaagaaat tattccttag atttgatcta gatgaagaaa tggggccccc
     4981 tggcagcagc ccaacaccag agcctccaca aaaagaagtg cagaaggaag gggctgcgga
     5041 cccaaagcag gtcggggagc ccagcgggca gaagaccctt gatggatcct cacggtctgc
     5101 agagctcgtc ccccagggcg aggatgattc tgagtatttg gagagagacg cccctgcagg
     5161 agatgctggg aggtggctgc gtgtcagctt tggtttgttt ggcagcgttt gggtgaacga
     5221 tttctccaga gccaagaaag ccaacaagag gggggactgg agggaccctt ccccgaggtt
     5281 ggtcctgcac tttggtggtg gtggcttcct ggcattttat aattgtcagt tgtcttggag
     5341 ctcttcccca gtggtcacac ccacctgtga catcctgtct gagaagttcc atcgaggaca
     5401 agccttagaa gctctaggcc aggctcagcc tgtctgctat acactgctgg accagagata
     5461 cttctcaggg ctagggaaca tcattaagaa tgaagccttg tacagagctg ggatccatcc
     5521 cctttctctc ggttcagtcc tgagtgcctc gcgtcgggag gtcctggtgg atcacgtggt
     5581 ggagttcagt acagcctggc tgcagggcaa gttccaaggc agaccgcagc acacacaggt
     5641 ctaccagaaa gaacagtgcc ctgctggcca ccaggtcatg aaggaggcgt ttgggcccga
     5701 agatgggtta cagaggctca cctggtggtg cccgcagtgc cagccccagt tgtcagagga
     5761 gccagagcag tgccagttct ccggtgccgc caccatgatc gatggcggag gcggatctga
     5821 tccaaaaaag aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa
     5881 gagaaaggta ggatctaccg gatctagaaa cgatggtggt ggtggaagcg ggggtggggg
     5941 cagcggtgga gggggaagcg ggcgcgccgg gatcctcccc cccaagaaaa agaggaaggt
     6001 atctagaggc cgcagccgcc ttttggaaga ttttcgaaac aaccggtacc ccaatttaca
     6061 actgcgggag attgctggac atataatgga attttcccaa gaccagcatg ggtccagatt
     6121 cattcagctg aaactggagc gtgccacacc agctgagcgc cagcttgtct tcaatgaaat
     6181 cctccaggct gcctaccaac tcatggtgga tgtgtttggt aattacgtca ttcagaagtt
     6241 ctttgaattt ggcagtcttg aacagaagct ggctttggca gaacggattc gaggccacgt
     6301 cctgtcattg gcactacaga tgtatggcag ccgtgttatc gagaaagctc ttgagtttat
     6361 tccttcagac cagcagaatg agatggttcg ggaactagat ggccatgtct tgaagtgtgt
     6421 gaaagatcag aatggcaatc acgtggttca gaaatgcatt gaatgtgtac agccccagtc
     6481 tttgcaattt atcatcgacg cgtttaaggg acaggtattt gccttatcca cacatcctta
     6541 tggctgccga gtgattcaga gaatcctgga gcactgtctc cctgaccaga cactccctat
     6601 tttagaggag cttcaccagc acacagagca gctggtacag gatcaatatg gaaattatgt
     6661 aatccaacat gtactggagc acggtcgtcc tgaggataaa agcaaaattg tagcagaaat
     6721 ccgaggcaat gtacttgtat tgagtcagca caaatttgca agcaatgttg tggagaagtg
     6781 tgttactcac gcctcacgta cggagcgcgc tgtgctcatc gacgaggtgt gcaccatgaa
     6841 cgacggtccc cacagtgcct tatacaccat gatgaaggac cagtatgcca actacgtggt
     6901 ccagaagatg attgacgtgg cggagccagg ccagcggaag atcgtcatgc ataagatccg
     6961 gccccacatc gcaactcttc gtaagtacac ctatggcaag cacattctgg ccaagctgga
     7021 gaagtactac atgaagaacg gtgttgactt aggggaccca aagaagaagc gcaaagtgga
     7081 tcctaaaaag aaaagaaagg taggcggccg cgggggaggc ggttccggtg gcggcggaag
     7141 cggaggtgga ggatcagggc cggccgaact gcccacctgc agctgtcttg atcgagttat
     7201 acaaaaagac aaaggcccat attatacaca ccttggggca ggaccaagtg ttgctgctgt
     7261 cagggaaatc atggagaata ggtatggtca aaaaggaaac gcaataagga tagaaatagt
     7321 agtgtacacc ggtaaagaag ggaaaagctc tcatgggtgt ccaattgcta agtgggtttt
     7381 aagaagaagc agtgatgaag aaaaagttct ttgtttggtc cggcagcgta caggccacca
     7441 ctgtccaact gctgtgatgg tggtgctcat catggtgtgg gatggcatcc ctcttccaat
     7501 ggccgaccgg ctatacacag agctcacaga gaatctaaag tcatacaatg ggcaccctac
     7561 cgacagaaga tgcaccctca atgaaaatcg tacctgtaca tgtcaaggaa ttgatccaga
     7621 gacttgtgga gcttcattct cttttggctg ttcatggagt atgtacttta atggctgtaa
     7681 gtttggtaga agcccaagcc ccagaagatt tagaattgat ccaagctctc ccttacatga
     7741 aaaaaacctt gaagataact tacagagttt ggctacacga ttagctccaa tttataagca
     7801 gtatgctcca gtagcttacc aaaatcaggt ggaatatgaa aatgttgccc gagaatgtcg
     7861 gcttggcagc aaggaaggtc gtcccttctc tggggtcact gcttgcctgg acttctgtgc
     7921 tcatccccac agggacattc acaacatgaa taatggaagc actgtggttt gtaccttaac
     7981 tcgagaagat aaccgctctt tgggtgttat tcctcaagat gagcagctcc atgtgctacc
     8041 tctttataag ctttcagaca cagatgagtt tggctccaag gaaggaatgg aagccaagat
     8101 caaatctggg gccatcgagg tcctggcacc ccgccgcaaa aaaagaacgt gtttcactca
     8161 gcctgttccc cgttctggaa agaagagggc tgcgatgatg acagaggttc ttgcacataa
     8221 gataagggca gtggaaaaga aacctattcc ccgaatcaag cggaagaata actcaacaac
     8281 aacaaacaac agtaagcctt cgtcactgcc aaccttaggg agtaacactg agaccgtgca
     8341 acctgaagta aaaagtgaaa ccgaacccca ttttatctta aaaagttcag acaacactaa
     8401 aacttattcg ctgatgccat ccgctcctca cccagtgaaa gaggcatctc caggcttctc
     8461 ctggtccccg aagactgctt cagccacacc agctccactg aagaatgacg caacagcctc
     8521 atgcgggttt tcagaaagaa gcagcactcc ccactgtacg atgccttcgg gaagactcag
     8581 tggtgccaat gcagctgctg ctgatggccc tggcatttca cagcttggcg aagtggctcc
     8641 tctccccacc ctgtctgctc ctgtgatgga gcccctcatt aattctgagc cttccactgg
     8701 tgtgactgag ccgctaacgc ctcatcagcc aaaccaccag ccctccttcc tcacctctcc
     8761 tcaagacctt gcctcttctc caatggaaga agatgagcag cattctgaag cagatgagcc
     8821 tccatcagac gaacccctat ctgatgaccc cctgtcacct gctgaggaga aattgcccca
     8881 cattgatgag tattggtcag acagtgagca catctttttg gatgcaaata ttggtggggt
     8941 ggccatcgca cctgctcacg gctcggtttt gattgagtgt gcccggcgag agctgcacgc
     9001 taccactcct gttgagcacc ccaaccgtaa tcatccaacc cgcctctccc ttgtctttta
     9061 ccagcacaaa aacctaaata agccccaaca tggttttgaa ctaaacaaga ttaagtttga
     9121 ggctaaagaa gctaagaata agaaaatgaa ggcctcagag caaaaagacc aggcagctaa
     9181 tgaaggtcca gaacagtcct ctgaagtaaa tgaattgaac caaattcctt ctcataaagc
     9241 attaacatta acccatgaca atgttgtcac cgtgtcccct tatgctctca cacacgttgc
     9301 ggggccctat aaccattggg tcatcgattg attaattaag aattcgaccc agctttcttg
     9361 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     9421 tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt
     9481 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     9541 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     9601 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     9661 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     9721 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     9781 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     9841 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     9901 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     9961 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    10021 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
    10081 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
    10141 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
    10201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
    10261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
    10321 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
    10381 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
    10441 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10501 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
    10561 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10621 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
    10681 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
    10741 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
    10801 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
    10861 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
    10921 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    10981 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    11041 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    11101 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    11161 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    11221 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    11281 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    11341 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
    11401 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    11461 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
    11521 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
    11581 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
    11641 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
    11701 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
    11761 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
    11821 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
    11881 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
    11941 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
    12001 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
    12061 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
    12121 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
    12181 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
    12241 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
    12301 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
    12361 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    12421 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    12481 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    12541 atcttc
//
