LOCUS       sequence-271797-       11090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11090)
  AUTHORS   Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z
  TITLE     Expanding C-T base editing toolkit with diversified cytidine 
            deaminases.
  JOURNAL   Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
  PUBMED    31399578
REFERENCE   2  (bases 1 to 11090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-9"; volume: "10(1):3612. doi"; pages: " 
            10.1038/s41467-019-11562-6"
COMMENT     Sequence Label: C-12-BE
FEATURES             Location/Qualifiers
     source          1..11090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     intron          409..1346
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1303..1323
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1374..1439
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1446..1466
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1491..1514,1515..1517,1518..5591)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1491..5591
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5592..5639
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5853..5918
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6750..7001
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7038..7286
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7299..7319
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7335..7388
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7389..7391,7392..8102)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7389..7391,7392..8102)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7692..7711)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8039..8060
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8131..8148)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8137..8344
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8353..8493
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8353..8482
                     /label=AAV2 ITR
     rep_origin      8568..9023
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8655..8674)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8865..8886
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9040..9059)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9159..9181
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9219..9237)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9305..9409
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9410..9478,9479..10270)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9628..9647)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10441..11029
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10930..10949
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      481 tccactggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      541 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      601 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      661 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      721 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
      781 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      841 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      901 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      961 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     1021 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1081 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1141 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1201 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1261 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1321 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggtgt cgtgagcgcc atggactata
     1381 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataaga
     1441 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt
     1501 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     1561 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     1621 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     1681 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     1741 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     1801 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     1861 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     1921 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     1981 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     2041 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     2101 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     2161 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     2221 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     2281 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     2341 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     2401 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     2461 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     2521 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     2581 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     2641 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     2701 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     2761 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     2821 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     2881 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     2941 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     3001 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     3061 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     3121 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     3181 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     3241 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     3301 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     3361 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     3421 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     3481 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     3541 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     3601 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     3661 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     3721 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     3781 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     3841 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     3901 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     3961 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga
     4021 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg
     4081 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
     4141 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     4201 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     4261 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     4321 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     4381 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     4441 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     4501 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     4561 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     4621 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     4681 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     4741 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     4801 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     4861 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     4921 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     4981 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     5041 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     5101 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     5161 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     5221 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     5281 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     5341 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     5401 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     5461 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     5521 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     5581 tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     5641 aattgggtgg aggaggtaca ggcggtggag gctcagcaga atacgtacga gctctgtttg
     5701 acttcaatgg gaatgacgag gaggatctcc cctttaagaa gggcgatatt ctccgcatca
     5761 gagataagcc cgaagaacaa tggtggaatg ccgaggatag cgaagggaaa aggggcatga
     5821 ttctggtgcc ttatgtggag aaatattccg gtgactacaa agaccatgat ggggattaca
     5881 aagaccacga catcgactac aaagacgacg acgataaaga attcggatcc agcgtgttcc
     5941 tgtataagaa gctgcccctg gacaccttcc tgttcgagtt caacaacctg gagtgggcct
     6001 acggccgcaa cagctgctac atctgcttca agctgaagcc catcctggcc accagcacca
     6061 ccggcaccac cgagagcgag ctgtggggct acgtgaccaa caagggcgag gtggccgacg
     6121 gcatcccctg cgagagcccc gagaagcgcg gcatgcacgc cgaggagctg ctgctggagg
     6181 agatgacccg ccacgtgcgc gagcacggcg gcgccagctt ctgcgtggag tggttcgcca
     6241 gctggagccc ctgcgaccgc tgcagcggcg tgctgctgcg ctggctgcgc gacgtgggcg
     6301 gcggacgcca ccgcctgcgc gtgtggttca gccgcatcta ccgcggcaac gtgggcgccg
     6361 tgcgcgccgg cctgcgccac ctgcgccgcg ccggcgtgca gctgggcgtg atggaccgcc
     6421 gccgccacga caactgcgtg cacgtgctgg tggacgccgc ccgcggcgac cccacccccc
     6481 tgtggctggt gcagtggcac agcaacgtgc cccgcgtgca gcgcgagttc gacaagatca
     6541 tggacgagaa ggtgcgcgag cccggctggt gctggtggtg ctggcgcccc tggatggccg
     6601 acgtgcccag caacgccagc ttcccccagc tgcccgtgaa cgacggcgcc atcgtggacc
     6661 gcgtggagaa gttcccctac ctgggcagcc tgctgatgac cagcagcggc ctggccgccg
     6721 aggtgagcat cgctagaggt acccaattca tgaccaacct ttccgacatc atagagaagg
     6781 aaacaggcaa acagttggtc atccaagagt cgatactcat gcttcctgaa gaagttgagg
     6841 aggtcattgg gaataagccg gaaagtgaca ttctcgtaca cactgcgtat gatgagagca
     6901 ccgatgagaa cgtgatgctg ctcacgtcag atgccccaga gtacaaaccc tgggctctgg
     6961 tgattcagga ctctaatgga gagaacaaga tcaagatgct atctggtggt tctggaggat
     7021 ctggtggttc tcaattcact aatctgtcag atattattga aaaggaaacc ggcaagcaac
     7081 tggttatcca ggaatccatc ctcatgctcc cagaggaggt ggaagaagtc attgggaaca
     7141 agccggaaag cgatatactc gtgcacaccg cctacgacga gagcaccgac gagaatgtca
     7201 tgcttctgac tagcgacgcc cctgaataca agccttgggc tctggtcata caggatagca
     7261 acggtgagaa caagattaag atgctctctg gtggttctcc caagaagaag aggaaagtcg
     7321 ctagcggcag tggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc
     7381 ctggcccagt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     7441 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     7501 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     7561 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     7621 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     7681 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     7741 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     7801 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     7861 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     7921 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     7981 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     8041 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     8101 aggcaattct aactagagct cgctgatcag cctcgactgt gccttctagt tgccagccat
     8161 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     8221 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     8281 ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg
     8341 gggagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     8401 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     8461 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     8521 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     8581 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     8641 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8701 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8761 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     8821 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8881 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     8941 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     9001 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     9061 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     9121 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     9181 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     9241 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     9301 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9361 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9421 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9481 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9541 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9601 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9661 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9721 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9781 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     9841 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     9901 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9961 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10021 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10081 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
    10141 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10201 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10261 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10321 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10381 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10441 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10501 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10561 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10621 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10681 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10741 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10801 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10861 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10921 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10981 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    11041 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
