LOCUS       sequence-271813-       10931 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-T base editing using LpCDA1L1_1 fused to C terminal of nCas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z
  TITLE     Expanding C-T base editing toolkit with diversified cytidine 
            deaminases.
  JOURNAL   Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6.
  PUBMED    31399578
REFERENCE   2  (bases 1 to 10931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-08-9"; volume: "10(1):3612. doi"; pages: " 
            10.1038/s41467-019-11562-6"
COMMENT     Sequence Label: C-7-BE
FEATURES             Location/Qualifiers
     source          1..10931
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     intron          409..1346
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1303..1323
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1374..1439
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1446..1466
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1491..1514,1515..1517,1518..5591)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1491..5591
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5592..5639
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5853..5918
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6591..6842
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             6879..7127
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7140..7160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7176..7229
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7230..7232,7233..7943)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7230..7232,7233..7943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7533..7552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7880..7901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(7972..7989)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7978..8185
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8194..8334
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   8194..8323
                     /label=AAV2 ITR
     rep_origin      8409..8864
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8496..8515)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8706..8727
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8881..8900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9000..9022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9060..9078)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9146..9250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9251..9319,9320..10111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9469..9488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10282..10870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10771..10790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacacca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
      301 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
      361 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
      421 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
      481 tccactggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
      541 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
      601 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
      661 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
      721 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
      781 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
      841 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
      901 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
      961 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     1021 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     1081 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     1141 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     1201 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     1261 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     1321 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggtgt cgtgagcgcc atggactata
     1381 aggaccacga cggagactac aaggatcatg atattgatta caaagacgat gacgataaga
     1441 tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt
     1501 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     1561 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     1621 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     1681 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     1741 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     1801 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     1861 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     1921 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     1981 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     2041 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     2101 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     2161 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     2221 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     2281 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     2341 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     2401 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     2461 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     2521 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     2581 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     2641 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     2701 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     2761 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     2821 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     2881 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     2941 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     3001 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     3061 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     3121 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     3181 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     3241 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     3301 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     3361 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     3421 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     3481 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     3541 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     3601 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     3661 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     3721 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     3781 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     3841 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     3901 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     3961 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga
     4021 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg
     4081 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
     4141 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     4201 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     4261 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     4321 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     4381 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     4441 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     4501 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     4561 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     4621 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     4681 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     4741 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     4801 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     4861 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     4921 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     4981 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     5041 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     5101 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     5161 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     5221 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     5281 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     5341 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     5401 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     5461 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     5521 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     5581 tgggaggcga caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagg
     5641 aattgggtgg aggaggtaca ggcggtggag gctcagcaga atacgtacga gctctgtttg
     5701 acttcaatgg gaatgacgag gaggatctcc cctttaagaa gggcgatatt ctccgcatca
     5761 gagataagcc cgaagaacaa tggtggaatg ccgaggatag cgaagggaaa aggggcatga
     5821 ttctggtgcc ttatgtggag aaatattccg gtgactacaa agaccatgat ggggattaca
     5881 aagaccacga catcgactac aaagacgacg acgataaaga attcggatcc gccaacgccg
     5941 agtacgtgcg cgtgggcgag aagctggaca gctgcacctt ccgcacccag ttcctgaact
     6001 accgccgcag ccgcagccgc cgctgctgcg tgatcttcga gctgaagcgc cagaacagcc
     6061 gcgtgcgctt ctggggctac gccatgaaca agccctggag caacgccgac gtgggcatcc
     6121 acgccgagtt cttctgcatc aagaaggtga agaagtacct gcgcaagaac cccggcatct
     6181 acaccatcaa ctggtacagc agctggagcc cctgcgccaa ctgcgccgag aagatcctga
     6241 actggtacaa caagaagctg atgggcaagg gccacaccct gaagatttgg gcctgcaagc
     6301 tgtacttcga gaacatcaag cgcaaccaga tcggcctgtg gaacctgcgc aacaacggcg
     6361 tgggcctggc catcatgctg ggcgagcact accagtggtg ctggaacaac tacatccaga
     6421 ccctgggccg caacctgaac gagaacaagt ggctgaaaaa gaccagcaac cgcgcccgca
     6481 cccgccgcag cgagctgagc atcatgatcc acgtgaagcg cctgcacacc gcccgcctgc
     6541 tgctgttcaa gcgcctgtgc ggctggttca gcgctagagg tacccaattc atgaccaacc
     6601 tttccgacat catagagaag gaaacaggca aacagttggt catccaagag tcgatactca
     6661 tgcttcctga agaagttgag gaggtcattg ggaataagcc ggaaagtgac attctcgtac
     6721 acactgcgta tgatgagagc accgatgaga acgtgatgct gctcacgtca gatgccccag
     6781 agtacaaacc ctgggctctg gtgattcagg actctaatgg agagaacaag atcaagatgc
     6841 tatctggtgg ttctggagga tctggtggtt ctcaattcac taatctgtca gatattattg
     6901 aaaaggaaac cggcaagcaa ctggttatcc aggaatccat cctcatgctc ccagaggagg
     6961 tggaagaagt cattgggaac aagccggaaa gcgatatact cgtgcacacc gcctacgacg
     7021 agagcaccga cgagaatgtc atgcttctga ctagcgacgc ccctgaatac aagccttggg
     7081 ctctggtcat acaggatagc aacggtgaga acaagattaa gatgctctct ggtggttctc
     7141 ccaagaagaa gaggaaagtc gctagcggca gtggagaggg cagaggaagt ctgctaacat
     7201 gcggtgacgt cgaggagaat cctggcccag tgagcaaggg cgaggagctg ttcaccgggg
     7261 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     7321 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     7381 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     7441 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     7501 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     7561 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     7621 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     7681 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     7741 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     7801 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     7861 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     7921 tcggcatgga cgagctgtac aaggcaattc taactagagc tcgctgatca gcctcgactg
     7981 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8041 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8101 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8161 aagagaatag caggcatgct ggggagcggc cgcaggaacc cctagtgatg gagttggcca
     8221 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     8281 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     8341 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     8401 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     8461 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     8521 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     8581 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     8641 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     8701 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     8761 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     8821 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     8881 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     8941 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     9001 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     9061 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9241 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9301 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9361 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9421 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9481 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9541 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9601 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9661 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9721 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9781 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9841 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9901 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9961 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
    10021 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
    10081 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
    10141 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
    10201 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
    10261 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
    10321 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
    10381 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
    10441 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
    10501 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10621 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10681 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10741 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10801 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10861 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
    10921 tgctcacatg t
//
