LOCUS       sequence-271992-       12284 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses epitope-tagged human TET3 WT in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12284)
  AUTHORS   Beck DB, Petracovici A, He C, Moore HW, Louie RJ, Ansar M, Douzgou 
            S, Sithambaram S, Cottrell T, Santos-Cortez RLP, Prijoles EJ, Bend 
            R, Keren B, Mignot C, Nougues MC, Ounap K, Reimand T, Pajusalu S, 
            Zahid M, Saqib MAN, Buratti J, Seaby EG, McWalter K, Telegrafi A, 
            Baldridge D, Shinawi M, Leal SM, Schaefer GB, Stevenson RE, Banka S,
            Bonasio R, Fahrner JA
  TITLE     Delineation of a Human Mendelian Disorder of the DNA Demethylation 
            Machinery: TET3 Deficiency.
  JOURNAL   Am J Hum Genet. 2020 Jan 3. pii: S0002-9297(19)30471-9. doi: 
            10.1016/j.ajhg.2019.12.007.
  PUBMED    31928709
REFERENCE   2  (bases 1 to 12284)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Am J Hum 
            Genet. 2020 Jan 3. pii: S0002-9297(19)30471-9. doi: 
            10.1016/j.ajhg.2019.12.007."
COMMENT     Sequence Label: pINTO-N3::hTET3
FEATURES             Location/Qualifiers
     source          1..12284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        363..742
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        743..946
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     897..917
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    948..966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    969..987
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     997..1021
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1109..1118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1118..1141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1151..1177
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(1187..1192,1193..1216,1217..1252,1253..1276)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             1286..1306
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    4112..4123
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     primer_bind     complement(6808..6825)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6814..7038
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7084..7512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7171..7190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7381..7402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7521..7541)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7526..7855
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7706..7841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7768..7787
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7903..7950
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7969..8343
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8473..8594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8510..8529)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8564..8583
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8643..8659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8643..8659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8656..8678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8667..8683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8691..8697,8698..8715,8716..8721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8736..8757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9506..9523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9677..10265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9757..9776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10436..11227,11228..11296))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11059..11078
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11297..11401)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11476..11495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atccaggaca gacttggctc gctggtgggc caggacagcg ggtatgtggg gggtcttccc
       61 aagaacgtta aggaaaagct gctgagcttg aagactttac aaagcgagct attcgaagtt
      121 gaaaaggaat ttcaggtgga gatgtttgag ctagagaaca agtttctgca gaagtacaag
      181 cccatttggg agcagcggtc caggatcatt tcaggacaag agcaacccaa gccggaacag
      241 atcgcaaagg gccaagagat tgtggagtca ctcaatgaga cggagttgca attgcatgaa
      301 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
      361 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
      421 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      481 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      541 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      601 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      661 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      721 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      781 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      841 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      901 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc ctatcagtga
      961 tagagatctc cctatcagtg atagagatcg tcgacgagct cgtttagtga accgtcagat
     1021 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     1081 cctccggact ctagcgttta aacttaaggc caccatggac tacaaggatg acgacgataa
     1141 gggcagcgga tacccatacg acgtgcctga ctacgccggc agcgctagcg catggtccca
     1201 tcctcaattc gagaaaggcg gcggatctgg cggtggaagc ggaggctccg cctggagcca
     1261 ccctcagttc gaaaagtccg gcagcgagaa cctgtacttc cagggctcca ccggtagcgg
     1321 taccgagctc ggatccgatg gaggactcga cagccagttt caggtgcccc tggccgtcca
     1381 gccggacctg ccaggccttt atgacttccc tcagcgccag gtgatggtag ggagcttccc
     1441 ggggtctggg ctctccatgg ctgggagtga gtcccaactc cgagggggtg gagatggtcg
     1501 aaagaaacgg aaacggtgtg gtacttgtga gccctgccgg cggctggaaa actgtggcgc
     1561 ttgcactagc tgtaccaacc gccgcacgca ccagatctgc aaactgcgaa aatgtgaggt
     1621 gctgaagaaa aaagtagggc ttctcaagga ggtggaaata aaggctggtg aaggagccgg
     1681 gccgtgggga caaggagcgg ctgtcaagac aggctcagag ctcagcccag ttgatggacc
     1741 tgttccaggt cagatggact cagggccagt gtaccatggg gactcacggc agctaagcgc
     1801 ctcaggggtg ccggtcaatg gtgctagaga gcccgctgga cccagtctgc tggggactgg
     1861 gggtccttgg cgggtagacc aaaagcccga ctgggaggct gccccaggcc cagctcatac
     1921 tgctcgcctg gaagatgccc acgatctggt ggccttttcg gctgtggccg aagctgtgtc
     1981 ctcttatggg gcccttagca cccggctcta tgaaaccttc aaccgtgaga tgagtcgtga
     2041 ggctgggaac aacagcaggg gaccccggcc agggcctgag ggctgctctg ctggcagcga
     2101 agaccttgac acactgcaga cggccctggc cctcgcgcgg catggtatga aaccacccaa
     2161 ctgcaactgc gatggcccag aatgccctga ctacctcgag tggctggagg ggaagatcaa
     2221 gtctgtggtc atggaaggag gggaggagcg gcccaggctc ccagggcctc tgcctcctgg
     2281 tgaggccggc ctcccagcac caagcaccag gccactcctc agctcagagg tgccccagat
     2341 ctctccccaa gagggcctgc ccctgtccca gagtgccctg agcattgcca aggaaaaaaa
     2401 catcagcttg cagaccgcca ttgccattga ggccctcaca cagctctcct ctgccctccc
     2461 gcagccttct cattccaccc cccaggcttc ttgccccctt cctgaggcct tgtcacctcc
     2521 tgcccctttc agatctcccc agtcttacct ccgggctccc tcatggcctg tggttcctcc
     2581 tgaagagcac tcatcttttg ctcctgatag ctctgccttc cctccagcaa ctcctagaac
     2641 tgagttccct gaagcctggg gcactgacac ccctccagca acgccccgga gctcctggcc
     2701 catgcctcgc ccaagccccg atcccatggc tgaactggag cagttgttgg gcagcgccag
     2761 tgattacatc cagtcagtat tcaagcggcc tgaggccctg cctaccaagc ccaaggtcaa
     2821 ggtggaggca ccctcttcct ccccggcccc ggccccatcc cctgtacttc agagggaggc
     2881 tcccacgcca tcctcggagc ccgacaccca ccagaaggcc cagaccgccc tgcagcagca
     2941 cctccaccac aagcgcagcc tcttcctaga acaggtgcac gacacctcct tccctgctcc
     3001 ttcagagcct tctgctcctg gctggtggcc cccaccaagt tcacctgtcc cacggcttcc
     3061 agacagacca cccaaggaga agaagaagaa gctcccaaca ccagctggag gtcccgtggg
     3121 aacggagaaa gctgcccctg ggatcaagcc cagtgtccga aagcccattc agatcaagaa
     3181 gtccaggccc cgggaagcac agcccctctt cccacctgtc cgacagattg tcctggaagg
     3241 gcttaggtcc ccagcctccc aggaagtgca ggctcatcca ccggcccctc tgcctgcctc
     3301 acagggctct gctgtgcccc tgcccccaga accttctctt gcgctatttg cacctagtcc
     3361 ctccagggac agcctgctgc cccctactca ggaaatgagg tcccccagcc ccatgacagc
     3421 cttgcagcca ggctccactg gccctcttcc ccctgccgat gacaagctgg aagagctcat
     3481 ccggcagttt gaggctgaat ttggagatag ctttgggctt cccggccccc cttctgtgcc
     3541 cattcaggac cccgagaacc agcaaacatg tctcccagcc cctgagagcc cctttgctac
     3601 ccgttccccc aagcaaatca agattgagtc ttcgggggct gtgactgtgc tctcaaccac
     3661 ctgcttccat tcagaggagg gaggacagga ggccacaccc accaaggctg agaacccact
     3721 cacacccacc ctcagtggct tcttggagtc acctcttaag tacctggaca cacccaccaa
     3781 gagtctgctg gacacacctg ccaagagagc ccaggccgag ttccccacct gcgattgcgt
     3841 cgaacaaata gtggagaaag atgaaggtcc atattatact cacttgggat ctggccccac
     3901 ggtcgcctct atccgggaac tcatggagga gcggtatgga gagaagggga aagccatccg
     3961 gatcgagaag gtcatctaca cggggaagga gggaaagagc tcccgcggtt gccccattgc
     4021 aaagtgggtg atccgcaggc acacgctgga ggagaagcta ctctgcctgg tgcggcaccg
     4081 ggcaggccac cactgccaga acgctgtgat cgtcatcctc atcctggcct gggagggcat
     4141 tccccgtagc ctcggagaca ccctctacca ggagctcacc gacaccctcc ggaagtatgg
     4201 gaaccccacc agccggagat gcggcctcaa cgatgaccgg acctgcgctt gccaaggcaa
     4261 agaccccaac acctgtggtg cctccttctc ctttggttgt tcctggagca tgtacttcaa
     4321 cggctgcaag tatgctcgga gcaagacacc tcgcaagttc cgcctcgcag gggacaatcc
     4381 caaagaggaa gaagtgctcc ggaagagttt ccaggacctg gccaccgaag tcgctcccct
     4441 gtacaagcga ctggcccctc aggcctatca gaaccaggtg accaacgagg aaatagcgat
     4501 tgactgccgt ctggggctga aggaaggacg gcccttcgcg ggggtcacgg cctgcatgga
     4561 cttctgtgcc cacgcccaca aggaccagca taacctctac aatgggtgca ccgtggtctg
     4621 caccctgacc aaggaagaca atcgctgcgt gggcaagatt cccgaggatg agcagctgca
     4681 tgttctcccc ctgtacaaga tggccaacac ggatgagttt ggtagcgagg agaaccagaa
     4741 tgcaaaggtg ggcagcggag ccatccaggt gctcaccgcc ttcccccgcg aggtccgacg
     4801 cctgcccgag cctgccaagt cctgccgcca gcggcagctg gaagccagaa aggcagcagc
     4861 cgagaagaag aagattcaga aggagaagct gagcactccg gagaagatca agcaggaggc
     4921 cctggagctg gcgggcatta cgtcggaccc aggcctgtct ctgaagggtg gattgtccca
     4981 gcaaggcctg aagccctccc tcaaggtgga gccgcagaac cacttcagct ccttcaagta
     5041 cagcggcaac gcggtggtgg agagctactc ggtgctgggc aactgccggc cctccgaccc
     5101 ttacagcatg aacagcgtgt actcctacca ctcctactat gcacagccca gcctgacctc
     5161 cgtcaatggc ttccactcca agtacgctct cccgtctttt agctactatg gctttccatc
     5221 cagcaacccc gtcttcccct ctcagttcct gggtcctggt gcctgggggc atagtggcag
     5281 cagtggcagt tttgagaaga agccagacct ccacgctctg cacaacagcc tgagcccggc
     5341 ctacggtggt gctgagtttg ccgagctgcc cagccaggct gttcccacag acgcccacca
     5401 ccccactcct caccaccagc agcctgcgta cccaggcccc aaggagtatc tgcttcccaa
     5461 ggccccccta ctccactcag tgtccaggga cccctccccc tttgcccaga gctccaactg
     5521 ctacaacaga tccatcaagc aagagccagt agacccgctg acccaggctg agcctgtgcc
     5581 cagagacgct ggcaagatgg gcaagacacc tctgtccgag gtgtctcaga atggaggacc
     5641 cagtcacctt tggggacagt actcaggagg cccaagcatg tcccccaaga ggactaacgg
     5701 tgtgggtggc agctggggtg tgttctcgtc tggggagagt cctgccatcg tccctgacaa
     5761 gctcagttcc tttggggcca gctgcctggc cccttcccac ttcacagatg gccagtgggg
     5821 gctgttcccc ggtgaggggc agcaggcagc ttcccactct ggaggacggc tgcgaggcaa
     5881 accgtggagc ccctgcaagt ttgggaacag cacctcggcc ttggctgggc ccagcctgac
     5941 tgagaagccg tgggcgctgg gggcagggga tttcaactcg gccctgaaag gtagtcctgg
     6001 gttccaagac aagctgtgga accccatgaa aggagaggag ggcaggattc cagccgcagg
     6061 ggccagccag ctggacaggg cctggcagtc ctttggtctg cccctgggat ccagcgagaa
     6121 gctgtttggg gctctgaagt cagaggagaa gctgtgggac cccttcagcc tggaggaggg
     6181 gccggctgag gagcccccca gcaagggagc ggtgaaggag gagaagggcg gtggtggtgc
     6241 ggaggaggaa gaggaggagc tgtggtcgga cagtgaacac aacttcctgg acgagaacat
     6301 cggcggcgtg gccgtggccc cagcccacgg ctccatcctc atcgagtgtg cccggcggga
     6361 gctgcacgcc accacgccgc ttaagaagcc caaccgctgc caccccaccc gcatctcgct
     6421 ggtcttctac cagcacaaga acctcaacca gcccaaccac gggctggccc tctgggaagc
     6481 caagatgaag cagctggcgg agagggcacg ggcacggcag gaggaggctg cccggctggg
     6541 cctgggccag caggaggcca agctctacgg gaagaagcgc aagtgggggg gcactgtggt
     6601 tgctgagccc cagcagaaag agaagaaggg ggtcgtcccc acccggcagg cactggctgt
     6661 gcccacagac tcggcggtca ccgtgtcctc ctatgcctac acgaaggtca ctggccccta
     6721 cagccgctgg atcctgtgaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta
     6781 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     6841 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     6901 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     6961 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7021 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7081 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7141 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7201 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7261 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7321 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7381 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7441 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7501 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7561 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7621 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7681 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7801 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7861 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7921 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     7981 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     8041 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     8101 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     8161 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     8221 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     8281 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8341 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8401 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8461 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8521 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8581 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8701 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8821 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc tagtctgcaa
     8881 tgagttcgag ttggcgcgcc tgtcattcta aatctctctt tcagcctaaa gctttttccc
     8941 cgtatccccc caggtgtctg caggctcaaa gagcagcgag aagcgttcag aggaaagcga
     9001 tcccgtgcca ccttccccgt gcccgggctg tccccgcacg ctgccggctc ggggatgcgg
     9061 ggggagcgcc ggaccggagc ggagccccgg gcggctcgct gctgccccct agcgggggag
     9121 ggacgtaatt acatccctgg gggctttggg agggggctgt ccccgtgagc tcccaggcgc
     9181 gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt
     9241 ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc
     9301 cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc gccggaccgg
     9361 agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta attacatccc
     9421 tgggggcttt gggggggggc tgtccccgtg agctcccagg cgcgccaact cggtactcgg
     9481 ggatttaatt aagctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     9541 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     9601 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     9661 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     9721 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     9781 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     9841 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     9901 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     9961 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10021 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10081 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10141 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10201 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10261 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10321 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10381 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    10441 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    10501 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    10561 ctgcaatgat accgcgagaa ccacgctcac cggctccaga tttatcagca ataaaccagc
    10621 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    10681 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    10741 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    10801 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    10861 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    10921 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    10981 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11041 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11101 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11161 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11221 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11281 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11341 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    11401 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
    11461 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
    11521 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
    11581 aggcttgacc gacaattgtt aattaagcta gcagtctgca atgagttcga gttggcgcgc
    11641 ctgtcattct aaatctctct ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct
    11701 gcaggctcaa agagcagcga gaagcgttca gaggaaagcg atcccgtgcc accttccccg
    11761 tgcccgggct gtccccgcac gctgccggct cggggatgcg gggggagcgc cggaccggag
    11821 cggagccccg ggcggctcgc tgctgccccc tagcggggga gggacgtaat tacatccctg
    11881 ggggctttgg gagggggctg tccccgtgag ctcccaggcg cgcctgtcat tctaaatctc
    11941 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
    12001 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
    12061 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
    12121 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tggggggggg
    12181 ctgtccccgt gagctcccag gcgcgccaac tcggtactcg gggatgctag cgcaccatca
    12241 acgaggagga catcttggct aaccaacccc tgttgttaca gtcc
//
