LOCUS       sequence-272199-        2014 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iFLinkC Linker cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Grawe A, Ranglack J, Weyrich A, Stein V
  TITLE     iFLinkC: an iterative functional linker cloning strategy for the 
            combinatorial assembly and recombination of linker peptides with 
            functional domains.
  JOURNAL   Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 
            10.1093/nar/gkz1210.
  PUBMED    31925441
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210."
COMMENT     Sequence Label: pL2- GL(GGGS)3ETG
FEATURES             Location/Qualifiers
     source          1..2014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     136..158
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(196..214)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(347..1162)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(347..1162)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1070..1089
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1154..1173
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1290..1877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1779..1798
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgcagcgagt cacccgaaga ccattttgga cgccagcagt gggtcttgga ggtggatccg
       61 gtggcggatc cggaggcgga tccgagacag ggcattgcat ccagccccga gcaaaaggag
      121 acccctgact agtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      181 gcgcgacacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      241 tggtttctta gacgtcaggt ggccttttcg gggaaatgtg cgcggaaccc ctatttgttt
      301 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
      361 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
      421 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
      481 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
      541 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
      601 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
      661 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagccagac
      721 gaaatacgcg gtcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
      781 ggaacaccgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
      841 ggaatgctgt tttccctggg atcgccgtgg tgagtaacca tgcatcatca ggagtacgga
      901 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
      961 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     1021 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     1081 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     1141 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     1201 ttattgtgaa ttctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     1261 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     1321 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1381 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1441 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1501 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1561 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1621 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1681 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1741 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1801 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatc tcgtcagggg
     1861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1921 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1981 ccgccaattg gctcgccgca gccgaacgac cgag
//
