LOCUS       sequence-272490-        4347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EMMA Kit # 1000000119 extension.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  TITLE     Lewin Lab Plasmids
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRDAX-VALENTIA-004   Lyn-mVenus//BsaI
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        158..537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        539..814
                     /label=chicken beta-actin promoter
     regulatory      834..843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1197..1216)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    1731..1852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1768..1787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1822..1841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      2653..2682
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      2704..3291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3192..3211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3303..3330)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(join(3354..4145,4146..4214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3977..3996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4215..4317)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(join(4318..4347,1..2))
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ctttctgcta tggaggtcag gtatgattta aaggtctcat aggtcttgta ttcagtctcg
       61 cactttagac atggtcttct gctgcccgtg gtcctctatt cgtcggatgt tctgtcgatt
      121 cttcctgtct atctggactg gatctgatat catcgtcgac attgattatt gactagttat
      181 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      241 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      301 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      361 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      421 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      481 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      541 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      601 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg ggggggggcg
      661 cgcgccaggc ggggcggggc ggggcgrggg gcggggcggg gcgaggcgga gaggtgcggc
      721 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      781 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgttgg gacgccacca
      841 tgggctgcat caagagcaag aggaaagacg tggaaaacgt cgagggcggc agcgtgtcaa
      901 agggagaaga gttgtttacc ggtgttgttc ccatactggt ggaactggat ggtgacgtca
      961 atggacacaa gtttagcgtt tctggcgaag gagaaggcga tgctacctat ggcaaactga
     1021 ccctcaaact gatctgtacg actggaaagt tgcctgtacc ttggccaaca ctggtcacaa
     1081 ccttgggcta tgggctgcaa tgctttgccc gttatcctga tcacatgaag caacatgact
     1141 tctttaagtc tgctatgcca gagggatatg tgcaggaacg gactatcttc ttcaaggacg
     1201 acggcaacta caagacgaga gctgaggtga aatttgaggg tgacacactg gtgaatcgca
     1261 tagagctgaa aggcatcgac ttcaaagaag atgggaatat tctcgggcat aaactcgagt
     1321 acaactacaa ttcccacaac gtgtacatca ctgccgataa gcagaagaat gggattaagg
     1381 cgaacttcaa gatccgacac aacatcgagg atggtggggt ccagcttgcc gaccattatc
     1441 agcagaacac acccattggg gatggaccag tcctgttgcc ggataaccac tacctcagtt
     1501 accagagcaa actgtccaaa gatcccaatg agaagaggga ccatatggta ctgctcgaat
     1561 tcgtgactgc agcaggcatt acccttggga tggatgaact ttataagtca ggctctgatt
     1621 aaggcgccag gtacatctcc aatacggcgt cttgcataga gaacacgatt agcacaccgg
     1681 ttacccacgt gatgcccgaa cggacccatg tcatgccaga gcgcactggt aacttgttta
     1741 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     1801 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     1861 gtcgtcgaat tcgagttggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt
     1921 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     1981 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     2041 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     2101 gggagggacg taattacatc cctgggggct ttgggagggg gctgtccccg tgagctccca
     2161 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
     2221 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     2281 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     2341 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     2401 atccctgggg gctttggggg ggggctgtcc ccgtgagctc ccaggcgcgc caactcggta
     2461 cctcacccga ttctcatcat ggagacacat caggacatct ccaataccgc catggaaacg
     2521 caccaagatg cgactgctct tctgccagtg acggcattcg tctgcaagat taacggcata
     2581 cagaaagaaa cgatgagacc cattcaggac gagcctcaga ctccagcgta actggactgc
     2641 aatcaactca ctggctcacc ttcacgggtg ggcctttctt cggtagaaaa tcaaaggatc
     2701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     2761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga ggtaactggc
     2821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3241 cttgagcatc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3301 aacgcagaaa ggcccacccg aaggtgagcc aggtgattac atttgggccc tcattaccaa
     3361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3421 tgactccccg tcgtgtagat aactacgatg cgggagggct taccatctgg ccccagtgct
     3481 gcaatgatac cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3541 gccgggaggg ccgagcgcag aagtgatcct gcaactttat ccgcctccat ccagtctatt
     3601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3781 tccttcggtc ctccgatcgt tgccagaagt aagttggccg cagtgttatc actcatggtt
     3841 atggcagcac tgcataattc tcttactgtc atgccatccg tgagatgctt ttctgtgact
     3901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     4021 ggaaaacgtt cttcggggcg taaactctca aggatcttac cgctgttgag atccagttcg
     4081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4201 tgttgaatac tcattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg
     4261 cccggtagtg atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaagt
     4321 caaaagcctc cggtcggagg cttttga
//
